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Introduction

029063 Protein Toxin Crystals Stain

Usage:For Protein Toxin Crystals Stain.

Specification: 10ml

10ml

Advanced performance at ultra-low reaction volumes, delivering consistent, cost-effective PCR genotyping for high-throughput, miniaturised and automated workflows.

About

Step into the future of high-throughput PCR genotyping with PACE Nano! Backed by decades of expertise in high-throughput genotyping reagent manufacturing, our groundbreaking product is engineered specifically to excel in low and ultra-low volume reactions. Say goodbye to performance variability as PACE Nano Genotyping Master Mix and PACE Genotyping Assays raise the bar for reliability and performance in the field.

With superior cost-effectiveness and versatile compatibility, PACE Nano Genotyping Master Mix ensures consistent results across diverse platforms, samples, and markers, including 384- and 1536-well PCR, Array Tape™, SNPline™ and Gene Mathematica from HC Scientific, available from 3CR Bioscience, supporting reaction volumes as low as 0.8 µL. Join the revolution today and elevate your research with PACE Nano.

• Cost-Effective: Produce more high-quality data faster and at a much lower cost compared to competitors, reducing reagent expenses without compromising on quality.
• Optimised for Low and Ultra-Low Volume PCR Genotyping: PACE Nano Genotyping Master Mix is specifically tailored for enhanced performance in miniaturised PCR genotyping, for reaction volumes as low as 0.8 µL and for high-throughput and automated processing with accurate, reliable results.
• Consistent Data Across Assays: PACE Nano reduces variability in assay performance, enhancing the robustness and efficiency of high throughput, miniaturised and automated workflows.
• Backed by 20 Years of Expertise: Designed and manufactured with 20 years of experience in high-throughput genotyping reagents, ensuring reliability and quality.
• Scalability: Our PACE® genotyping reagents are platform agnostic, so you can adapt to changing throughput requirements effortlessly, ensuring flexibility and efficiency at every stage of your genotyping workflow.
• PACE Nano Global Product Support: Benefit from a stable supply chain and unparalleled global product support, tailored to your needs regardless of size or throughput. Supported by the highest quality-assurance processes, ensuring reliability and consistency.

REQUIRED COMPONENTS

• qPCR machine or Thermocycler + Fluorescent plate reader
• PCR plate or equivalent and appropriate optically clear seal
• Template DNA
• PCR-grade water
• Genotyping assays

qPCR machine or Thermocycler + Fluorescent plate reader
PCR plate or equivalent and appropriate optically clear seal
Template DNA
PCR-grade water
Genotyping assays

Overview

• DNA can be isolated and detected from as little as 100 µL of sputum
• Effective removal of PCR inhibitors
• Compatible with Norgen’s Saliva Collection and Preservation Device (Cat. RU49000)
• Sputum Liquification Buffer (Cat. 28289) can be purchased separately

This kit constitutes an all-in-one system for the rapid isolation of DNA from sputum samples. It allows for the isolation of bacterial or eukaryotic DNA from the sputum samples using spin-column chromatography based on Norgen’s proprietary resin. The kit includes all the reagents needed to process sputum DNA. The protocol can be completed in 30 minutes. Purified DNA is of the highest quality and free from inhibitors, and can be used in sensitive downstream applications including PCR and qPCR.

Background

A sputum specimen is the name given to the mucus which is expectorated from the lower airways. High quality sputum samples should contain very little saliva, as this may contaminate the sputum sample with oral bacteria. Sputum samples are typically evaluated to look for infections such as Moraxella catarrhalis, Mycobacterium tuberculosis, Streptococcus pneumoniae ;and Haemophilus influenza. Other pathogens can be detected in sputum as well, including HIV. In addition, sputum samples are useful in the detection of lung cancer or to evaluate chronic inflammation.

Details

Supporting Data

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Kit Specifications
Time to Complete DNA Isolation	< 1 hour
Average Yield of DNA from 1.0 mL*	75 μg
Average Purity (OD260/280)	1.8 – 2.1

* Average DNA yield will vary depending on the health status of the donor

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Norgen’s Sputum DNA Isolation Kit contains ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Component	Cat. 46200 (25 preps)
Slurry D	55 mL
Proteinase K in Storage Buffer	0.6 mL
Solution WN	4 mL
Wash Solution BE	9 mL
Elution Buffer B	8 mL
Mini Filter Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1