Bis-propargyl-PEG10 is reactive with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. By introducing PEG units into the molecule, the solubility of the compound in aqueous environment can be improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG10 is reactive with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. By introducing PEG units into the molecule, the solubility of the compound in aqueous environment can be improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-thiol is a crosslinker containing a propargyl group and thiol group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The thiol group reacts with maleimide, OPSS, vinylsulfone and transition metal surfaces including gold, silver, etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG4-thiol is a crosslinker containing a propargyl group and thiol group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The thiol group reacts with maleimide, OPSS, vinylsulfone and transition metal surfaces including gold, silver, etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s Preserved Blood RNA Purification Kit II provides a rapid method for the isolation and purification of total RNA from blood that has been preserved using PAXgene Blood RNA Tubes*. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Norgen’s proprietary resin provides superior affinity to the full size range of RNA molecules, resulting in large and small RNA (miRNA) purification with better linearity and sensitivity. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time RT-PCR, RT-PCR, Northern blotting, RNase protection and primer extension, expression profiling, miRNA cloning and amplification and Next Generation Sequencing.
* PAXgene Blood RNA Tubes are a product of PreAnalytiX GmbH. PAXgene Blood RNA Tubes are not provided.
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| Kit Specifications | |
| Maximum Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Time to Complete 10 Purifications | 30 minutes |
| Average Yield | 5 – 20 μg per 2.5 mL preserved human blood |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The DNase I should be stored at -20°C.
| Component | Cat. 43500 (50 preps) |
|---|---|
| NPX1 | 2 x 110 |
| NPX2 | 40 mL |
| NPX3 | 22 mL |
| NPX4 | 6 mL |
| NPX5 | 6 mL |
| DNase I | 1 vial |
| Mini Spin Columns | 50 |
| Collection Tubes | 50 |
| Elution Tubes (1.7 mL) | 50 |
| Product Insert | 1 |
Bis-PEG11-DBCO represents a homobifunctional PEG linker with DBCO moieties on either side that perform copper-free catalyzed click chemistry with azides. The PEG spacer imparts water-solubility and helps with fine-tuning DMPK properties. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-PEG11-DBCO represents a homobifunctional PEG linker with DBCO moieties on either side that perform copper-free catalyzed click chemistry with azides. The PEG spacer imparts water-solubility and helps with fine-tuning DMPK properties. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
