Bis-propargyl-PEG10 is reactive with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. By introducing PEG units into the molecule, the solubility of the compound in aqueous environment can be improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG10 is reactive with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. By introducing PEG units into the molecule, the solubility of the compound in aqueous environment can be improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
,
,
Product Description
High Purity DNA Purification Kit Fast Extract Isolate Nucleic Acids
Product parameters:
| Reagent component (WLD8201-ES,2Tubes/box) | ||
| Component | Specification | Quantity |
| LD-1 | 800μL＝0.8mL | 1 Tube |
| LD-2 | 200μL＝1.2mL | 1 Tube |
Product features and advantages:
| Product features | |
| Simplicity | The operation is simple, and the DNA template for amplification can be obtained in just one step (conventional 5min) |
| Security | Non-toxic environmental protection, room temperature transport and storage |
| Universality | The lysate can be used for isothermal nucleic acid amplification |
Product application:
It is used for pre-processing and nucleic acid release of swab samples and plant tissue samples, rapidly cracking all kinds of common samples without purification; The sample lysate can be directly used as a template for amplification reaction.
Tips: Its reverse combined with our constant temperature amplification reagent for nucleic acid detection.
| Sample processing type | |
| Liquid sample | Samples of plants or tissues |
| Serum, blood, saliva, ascites and other samples, swab leach solution | Brain, muscle, viscera, leaves, fruit and other samples, take liquid supernatant |
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from blood, tissue, culture cells, swab, blood spots using 96 plate |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells |
| Sample amount | |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | D311701 | D311702 |
| Purification Times | 1 x 96 | 4 x 96 |
| HiPure gDNA Plate | 1 | 4 |
| 96 well Plate (2.2ml) | 1 | 4 |
| 1.6ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| Silicon Seal Tape | 1 | 4 |
| Seal Film | 5 | 25 |
| Buffer ATL | 30 ml | 100 ml |
| Buffer AL | 30 ml | 100 ml |
| Buffer DW1 | 60 ml | 250 ml |
| Buffer GW2 | 50 ml | 2 x 100 ml |
| Proteinase K | 50 ml | 200 ml |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer AE | 30 ml | 120 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
| Name | CAT NO | Sample amount | Leukocyte protocol* | Colum type | Elutio volume | Average yield | Time per run |
| HiPure Blood DNA Mini Kit | D3111 | 10-200μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure Tissue&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 well plate | 3-8μg/200μl |
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 4 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.
ANSI/ SBS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot on bottom. ANSI/SBS footprint on top to accept all common microplates.
Integrated Cushion base for maximum recovery. Helps aid in set-up, robot positioning inconsistencies, and labware consumable inconsistencies
Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads
A040523
A040523KT (Kit for 0.2 ml tubes)
384-Well PCR
Maximum – 39 µL
Minimum – 5 µL
__________
96-Well PCR
Maximum – 60 µL (0.2 mL for some beads)
Minimum – 4 µL
Ideal magnet for all of your low volume needs. From Low elution 96-well PCR applications to 384-well, the X396 has you covered. No need to purchase two separate plates anymore. 96-well volumes as low as 4 µL are achieved from 96-well, PCR plates on the inside of the magnet, while the outside of each magnet will pull beads to well sides in 384-well plates.
