Bis-propargyl-PEG10 is reactive with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. By introducing PEG units into the molecule, the solubility of the compound in aqueous environment can be improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Bis-propargyl-PEG10 is reactive with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. By introducing PEG units into the molecule, the solubility of the compound in aqueous environment can be improved. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Exosomal RNA Isolation Kit (for the extraction of RNA from EVs that have been isolated using IZON’s qEV columns)
Product Info
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Product Info
Overview
Extract high quality & quantity total RNA including miRNA
No phenol step required; isolate all RNA in one fraction
Bind & elute all RNA irrespective of size or GC content, without bias
Very sensitive & linear down to a few cells without the need for carrier RNA
Isolate from a wide variety of specimens
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Available in a variety of formats to suit your needs
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
Norgen’s Exosomal RNA Isolation Kit (for the extraction of RNA from EV’s that have been isolated using IZON’s qEV columns) constitutes an all-in-one system for the isolation of exosomal RNA from exosomes previously isolated using IZON’s qEV columns or concentrated using IZON’s EV Concentration kit. This kit allows for the isolation of RNA from intact extracellular vesicles (EVs) where the purification is based on spin column chromatography that employs Norgen’s proprietary resin.
The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PR, reverse transcription PCR, Nothern blotting, RNase protection and primer extension, and expression array assays.
Details
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature (15–25°C). This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Available Carbohydrates, Dietary Fiber
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Limit of Detection:
0.5 g/100 g
Reaction Time (min):
~ 78 min
Application examples:
Food ingredients, food products and other materials.
The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.
All reagents stable for > 2 years after preparation
High purity / standardised enzymes employed
Only kit available
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Simple format
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The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.