Bis-propargyl-PEG6 has two propargyl groups which can participate in Click Chemistry reactions to yield a stable triazole linkage with azide compounds; copper is needed as a catalyst. The 6 units of PEG increase the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG6 has two propargyl groups which can participate in Click Chemistry reactions to yield a stable triazole linkage with azide compounds; copper is needed as a catalyst. The 6 units of PEG increase the hydrophilicity of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Product Description
20mins High Sensitivity Isothermal DNA Amplification Kit For Accurate Detection
Applicable equipment:
It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex;Source search and combine the target homology domain, at this time,a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | DNA Isothermal Amplification Kit EXO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(fluorescent type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
Fluorescent Probe:Require the suitable length is 46-52nt.
DNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
These kits provide a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from mammalian blood samples. These kits are supplied with an RBC (red blood cell) Lysis Buffer for selective removal of red blood cells and fractionation of leukocytes by centrifugation. Isolation of leukocyte RNA results in improved expression profiling and other downstream applications by removing the masking effects of some RNAs which are very abundant in whole blood, such as globin mRNAs. These kits are able to isolate total leukocyte RNA, including both large mRNA and all small RNA species containing microRNA (miRNA) and small silencing RNA (siRNA). The purified RNA is of the highest quality and can be used in a number of downstream applications.
Leukocyte RNA Purification Kit (Spin Column)
This kit provides a rapid method for the isolation and purification of total leukocyte RNA from mammalian blood samples in 40 minutes. Allowable blood input ranges from 10 μL to 2 mL or 3 x 106 Leukocytes
Leukocyte RNA Purification Plus Kit (Plus)
Norgen’s Leukocyte RNA Purification Plus Kit provides a rapid method for the isolation and purification of total leukocyte (white blood cell) RNA from up to 3 mL of mammalian blood samples. Complete 10 purifications in as little as 40 minutes.
Leukocyte RNA Purification 96-Well Kit (High Throughput)
Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Norgen’s kit allows for the isolation of total leukocyte RNA, including all small RNA species. The purified RNA is of the highest quality and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, northern blotting, RNase protection and primer extension, and expression array assays. Allowable blood input ranges from 10 μL to 1 mL or 1.5 x 106 Leukocytes.
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Click for expanded view
| Kit Specifications | |
| Maximum Column Binding Capacity | Up to 50 μg RNA |
| Maximum Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA <200 nt |
| Minimum Blood Input | 10 μL |
| Maximum Blood Input | 2 mL or 3 x 106 leukocytes |
| Time to Complete 10 Purifications | 40 minutes |
| Input Type | Blood from Human, Hamster, Mouse, Rabbit, and Rat |
| Average Yield Human Blood (500 μL) Hamster Blood (100 μL) | 1.5 μg 2.5 μg |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The RBC Lysis Buffer should be stored at 4°C upon arrival.
| Component | Cat. 21200 (50 preps) | Cat. 21250 (50 preps) | Cat. 37800 (2 x 96 preps) |
|---|---|---|---|
| RBC Lysis Buffer | 2 x 100 mL | 2 x 1 L | 2 x 90 mL |
| Buffer RL | 30 mL | 30 mL | – |
| Binding Solution | – | – | 2 x 40 mL |
| Wash Solution A | 38 mL | 38 mL | – |
| Wash Solution | – | – | 2 x 30 mL |
| Elution Solution A | 6 mL | 6 mL | – |
| Elution Solution | – | – | 2 x 20 mL |
| Enzyme Incubation Buffer | – | 6 mL | 1 |
| DNase I | – | 1 Vial | – |
| Mini Spin Columns | 50 | – | – |
| Lysate Homogenization Column | – | 50 | – |
| Single Cell RNA Column | – | 50 | – |
| RBC Lysis 96-Well Plate | – | – | 2 |
| 96-Well Filter Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| Collection Tubes | 50 | 100 | – |
| 96-Well Collection Plate | – | – | 2 |
| Elution Tubes (1.7 mL) | 50 | 50 | – |
| 96-Well Elution Plate | – | – | 2 |
| Product Insert | 1 | 1 | 1 |
DM750 Video Microscope
1.Ergonomic design,reduce the pressure of the operator,40cm in front of the screen, adjustable angle. Simplified Operation with wireless mouse.
2.Simple design of production line,CCD,LCD display,LED lighting, integrated built-in design.
3.Clear optical observation effect,high-definition display,resolution of 1080P
4.Powerful software operation,can measure,store,photograph,
compare.
5.Magnification:The standard configuration is 10.3X-73.6X; it can be extended to 5.1X-368X.
6.It supports 2D and 3D observation methods, 360 degree rotation, omni-directional 3D observation,large field of view and large depth of field.
Specification
| Video Microscope | DM750 | |
| Magnification | 10.3X–73.6X | ● |
| Eyepiece | 0.4X(1X for option) | ● |
| Objective | 0.7X-5X Zoom Range | ● |
| Zoom Ratio | 01:07.1 | ● |
| Auxiliary Lens | 0.5X,0.7X,2X For option | ◯ |
| Input | 100-240V | ● |
| Output | 12V/2A | ● |
| Working Distance | 100mm | ● |
| Head Size | 197mmX192mmX95mm | ● |
| HDMI Cable with Ethernet | HDMI Alloy Plate, Built-in chip, Synchronized output | ● |
| Display | 11.6 inch LCD screen | ● |
| Mouse | Wireless mouse | ● |
| Base | B11 Base | ● |
| Adapter | Input100-240V, Output12V/2A | ● |
| 3D lens | 360 degree rotation omni-directional 3D observation | ◯ |
Note:●Standard Outfit ◯ Optional
Microscope same as centrifuge, which it is basic instrument in laboratory, Yingtai instrument acquires a new factory specilized in microscope. We can make various microscope, any requirement, can check with us.
