Bis-propargyl-PEG9 is crosslinking reagent containing two propargyl groups. These functional groups enable the formation of triazole linkages with azide-bearing compounds with the presence of copper catalyst. The hydrophilicity of the molecule is improved because of the PEG chain. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Bis-propargyl-PEG9 is crosslinking reagent containing two propargyl groups. These functional groups enable the formation of triazole linkages with azide-bearing compounds with the presence of copper catalyst. The hydrophilicity of the molecule is improved because of the PEG chain. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Propargyl-PEG10-t-butyl ester
Product Info
Document
Product Info
Propargyl-PEG10-t-butyl ester is a crosslinking reagent that can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG10-t-butyl ester is a crosslinking reagent that can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The carboxyl group can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
User-friendly gel cassette:
Numbered and framed wells for sample loading
With cassette opener for easy use
Enhanced gel performance:
Enhanced band sharpness
Better resolution of small proteins
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Document
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from bacteria culture
Applications
RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Bacteria culture
Sample amount
Bacteria: <10^9
Elution volume
≥30μl
Time per run
≤40 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Fast – several samples can be extracted in 30 minutes
High purity – the purified RNA can be directly used in various downstream applications
High recovery – RNA can be recovered at the level of PG
Good repeatability – silica gel column purification technology can obtain ideal results every time
Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
Sufficient components – the kit contains carried lysozyme and glass beads
Kit Contents
Contents
R418101
R418102
R418103
Purification Times
10 Preps
50 Preps
250 Preps
gDNA Filter Mini Columns
10
50
250
HiPure RNA Mini Columns
10
50
250
2ml Collection Tubes
20
100
500
Glass Beads (0.1-0.6mm)
10 g
30 g
150 g
Plastic spoon
2
4
10
Lysozyme
20 mg
90 mg
400 mg
Protease Dissolve Buffer
1.8 ml
1.8 ml
10 ml
Buffer TE
1.8 ml
1.8 ml
5 ml
Buffer STL
5 ml
20 ml
90 ml
Buffer RLC
10 ml
30 ml
150 ml
Buffer RW1
10 ml
50 ml
250 ml
Buffer RW2*
5 ml
20 ml
2 x 50 ml
RNase Free Water
1.8 ml
10 ml
30 ml
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions. Due to the lack of antibacterial agents, RNase Free Water may be contaminated by bacterial or fungal when placed or operated at room temperature. It is recommended to pack and store at 2-8°C to reduce contamination.
Document
HiPure Bacterial RNA Kit uses silica gel column purification to simplify the extraction. The whole process does not require phenol chloroform extraction and time-consuming alcohol precipitation. The kit is suitable for efficiently extracting RNA from various bacterial samples. The purified RNA can be directly used for RT-PCR, Northern hybridization, etc. The kit has included lysozyme and glass beads, which can be used to treat gram-negative bacteria which is easy to be lysed, as well as gram-positive bacteria which is hard to be lysed, including enterococcus faecalis, staphylococcus, etc.