Bis-propargyl-PEG9 is crosslinking reagent containing two propargyl groups. These functional groups enable the formation of triazole linkages with azide-bearing compounds with the presence of copper catalyst. The hydrophilicity of the molecule is improved because of the PEG chain. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-propargyl-PEG9 is crosslinking reagent containing two propargyl groups. These functional groups enable the formation of triazole linkages with azide-bearing compounds with the presence of copper catalyst. The hydrophilicity of the molecule is improved because of the PEG chain. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-FUCOSE
SKU: 700004288
100 assays (manual) / 1000 assays (microplate) / 1020 assays (auto-analyser)
| Content: | 100 assays (manual) / 1000 assays (microplate) / 1020 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | L-Fucose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.5 to 100 µg of L-fucose per assay |
| Limit of Detection: | 0.68 mg/L |
| Reaction Time (min): | ~ 10 min |
| Application examples: | L-Fucose is present as the main component in fucoidan (a marine polysaccharide), foods, pharmaceuticals and other materials (e.g. biological samples, etc.). |
| Method recognition: | Novel method |
The L-Fucose test kit is a simple, rapid and reliable method, for the measurement and analysis of L-Fucose in plant extracts, biological samples and other materials. This kit can be used in the measurement of α-fucosidases that do not act on chromogenic substrates.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our full range of monosaccharide and disaccharide assay kits.
Advantages
The L-Fucose test kit is a simple, rapid and reliable method, for the measurement and analysis of L-Fucose in plant extracts, biological samples and other materials. This kit can be used in the measurement of α-fucosidases that do not act on chromogenic substrates.
The Multiplexing Index Primers contain primer mix for multiplexing library samples for Next Generation Sequencing (NGS) on the illumina platform. Multiplexing of NGS library samples will reduce sequencing costs by pooling multiple NGS libraries into a single flow cell lane.
With library multiplexing, unique index sequence is added to individual sample during NGS library preparation. Therefore, each DNA molecule can be identified after pooling of multiple samples based on the index information they have.
Each of our index primers contains a unique index sequence with 6 bases that can be used to identify libraries. Library multiplexing up to 48 samples is possible.
Multiplexing Index Primers (illumina platform): Even distribution of 48 samples using index primers. 48 libraries were made using the BioDynami NGS DNA Library Prep Kit (Cat. # 30009) and the BioDynami Multiplexing Index Primers (Cat. # 30072). Libraries were pooled at equal concentration and sequenced on the illumina HiSeq 2500. The numbers of reads from 48 libraries were analyzed.
List of index sequence for the primers (each of the index primer mix contains universal primer and one of the index primers). Index number and the index sequence are listed.
List of indexes can be downloaded Here.
Sequence of the final library with index location:
The Multiplexing Index Primers contain primer mix for multiplexing library samples for Next Generation Sequencing (NGS) on the illumina platform. Multiplexing of NGS library samples will reduce sequencing costs by pooling multiple NGS libraries into a single flow cell lane.
The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
QPCR is the best method for library quantification. Our reagent only amplifies library molecules that will be used for subsequent emPCR, and is optimized for amplification of various samples. Our reagent is compatible with commercial SYBR Green based QPCR reagents. Quantification of library concentration is achieved by comparison with a standard curve generated from DNA Standards.
The kit comprises DNA Standards (six 10-fold dilutions) and a primer mix.
NGS Library Quantification Standards with PCR Primers (Ion Torrent platform): real time quantitative PCR curve of the standards.
The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.
