Both high molecular weight DNA (greater than 1 kb in size; mostly cell associated) and the smaller DNA (150 – 250 bp; derived from the circulation) is effectively isolated and purified using a rapid and convenient spin column protocol. This kit can be used to isolate DNA from a broad range of viruses in urine as well. Salts, metabolic wastes, proteins and other contaminants are removed to yield inhibitor-free DNA for use in sensitive applications. The DNA is of excellent quality for various downstream applications such as PCR, qPCR and DNA fingerprinting, methylation studies and more.
This kit is fully compatible with Norgen’s Urine Collection and Preservation Tubes.
Urine DNA Isolation Kit
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 50 μL to 1.75 mL of urine. Multiple samples can be processed in 30 minutes.
Urine DNA Isolation Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 3 mL to 25 mL. Multiple samples can be processed in 30 minutes. Multiple samples can be processed in 45 minutes.
Urine DNA Isolation Maxi Kit (Slurry Format)
This kit provides a fast, reliable and simple procedure for isolating DNA from urine volumes ranging from 25 mL of urine up to 80 mL. Multiple samples can be processed in 45 minutes.
Background
DNA found in urine can be divided into 2 basic categories. The larger species, genomic-DNA (gDNA), is generally greater than 1 kb in size, and appears to be derived mainly from exfoliated cells. The second species is smaller, generally between 150 and 250 bp (apoptotic-DNA), and derives, at least in part, from the circulation. The second species is also considered as an RNA/DNA hybrid as reported by Halicka et al. (2000). Both types of DNA can be isolated reliably using this kit.
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| Kit Specifications | |
| Volume of Urine Processed | 1.75 mL |
| Average Yield* | Up to 50 ng |
| Size of DNA Purified | Large (> 1 kb) and small (150-250 bp) |
| Time to Complete 10 Purifications | 30 minutes hands-on time (plus a 1 hour incubation) |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
| Component | Cat. 18100 (50 preps) | Cat. 48800 (50 preps) | Cat. 50100 (50 preps) |
|---|---|---|---|
| Binding Solution K | 15 mL | – | – |
| Slurry B1 | – | 18 mL | 18 mL |
| Proteinase K in Storage Buffer | 2 mL | – | – |
| Pronase K in Storage Buffer | 2 mL | – | – |
| Soluton WN | 9 mL | – | – |
| Binding Buffer A | – | – | 50 mL |
| Lysis Buffer A | – | 30 mL | 30 mL |
| Wash Solution A | – | 38 mL | 38 mL |
| Wash Solution B | 30 mL | – | – |
| Wash Solution D | 9 mL | – | – |
| Binding Solution K | 15 mL | – | – |
| Elution Buffer B | 15 mL | 15 mL | 15 mL |
| Micro Spin Columns | 50 | – | – |
| Mini Filter Spin Columns | – | 50 | 50 |
| Collection Tubes | 50 | 50 | 50 |
| Elution Tubes (1.7 mL) | 100 | 100 | 100 |
| Product Insert | 1 | 1 | 1 |
Norovirus is a single-stranded, non-enveloped RNA virus belonging to the Caliciviridae family. Norovirus is considered the major causative agent of non-bacterial gastroenteritis in the world. The main channel of transmittance is via contaminated food or water, as well as human-to-human contact. Most Noroviruses infecting humans belong to genogroup I and II (particularly genotype 4). Norovirus is very stable in the environment and is resistant to some surface disinfectants such as alcohols and detergents. Moreover, it is very difficult to culture Norovirus in vitro, making its identification by standard microbiological assays challenging.
NoV TaqMan RT-PCR Kit, 100 reactions
NoV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM41450 (100 preps) | Cat. TM41410 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| NoV Primer & Probe Mix | 280 μL | 280 μL |
| NoV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
