Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
Usages:
Pancreatic digest of casein obtained with a process to maintain a high level of vitamins and growth factors, it is particularly recommended for a rapid growth and detection of microorganisms present in low concentrations.
Technical specification:
Total nitrogen(TN)—————————≥11.5%
Amino nitrogen(AN) ————————≥3.0%
Sulphuri ash———————————–≤15.0%
Loss on drying——————————–≤6.0%
Chlorides—————————————≤2.0%
PH(5% solution)——————————7.0±0.5
Solubility in 5% water ———————–complete
Nitrites——————————————negative
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.
Specifications: 500g/bottle; 10kg/bag
500g/bottle;10KG/bag
Norgen’s Legionella sp. TaqMan PCR Kit is designed for the detection of Legionella sp. specific DNA in a real-time PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of Legionella sp. specific DNA is based on TaqMan PCR providing a simple, reliable and rapid result for the detection of Legionella sp. infection. Norgen’s Legionella sp. TaqMan PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The Legionella sp. TaqMan PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.
Legionella sp. TaqMan PCR Kit, 100 reactions
Legionella sp. TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM64450 (100 rxns) | Cat. TM64410 (100 rxns) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 mμL | – |
| Legionella sp. Primer & Probe Mix | 280 μL | 3 x 70 μL |
| Legionella sp. Positive Control | 150 μL | 3 x 50 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |