Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
[PM1600] ExcelBand™ All Blue Regular Range Plus Protein Marker (9-180 kDa), 250 μl x 2
Product Info
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Product Info
Description
The PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker is a blue protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).
The PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Features
Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
Two enhanced bands — 72 kDa and 25 kDa
Contents
Approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Quality Control
Under suggested conditions, PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker resolves 11 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane.
Storage
4°C for 3 months -20°C for long term storage
Document
The PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker is a blue protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-Glycine buffer).
The PM1600 ExcelBand™ All Blue Regular Range Plus Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Saxitoxins (STXs) are naturally occurring alkaloids produced by some marine dinoflagellates and by strains of various species of freshwater cyanobacteria. Saxitoxin is one of the prevalent paralytic shellfish toxins (PSTs). It belongs to a family of potent neurotoxins with a molecular weight around 300 Da. Saxitoxin and its derivatives are alkaloids composed of a tetrahydropurine ring system with a highly polar guanidinium group. Due to their significant toxicity, saxitoxins are closely monitored in marine environments where they can accumulate during harmful algal blooms (HABs). In 2009 an action level of 0.6ppb (600ppt) was recommended by EFSA as a guidance framework have been established to manage saxitoxin levels in water.
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Screening of saxitoxin in Freshwater Streams and Source Water samples as low as 70 ppt
Format: 10 tests (5 tests/5 control)
Water Sample Bottles provided
Run Time: 15 Minutes
For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method.
Principle and Interpretation
MUG permits the rapid detection of Escherichia coli when the medium is observed for fluorescence using a UV light. MUG is hydrolyzed by the enzyme β-glucuronidase which is produced by E. coli to yield a fluorescent end product 4- methylumbelliferone. Trypticase provides the essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains the osmotic equilibrium. The medium has a strong buffering system to control the pH in the presence of fermentative action. The bile salts inhibit gram-positive bacteria especially the Bacillus species and faecalStreptococci.
Formulation
Ingredients
/liter
Trypticase or tryptose
20 g
Bile salts No. 3
1.5 g
Lactose
5 g
K2HPO4
4 g
KH2PO4
1.5 g
NaCl
5 g
4-methylumbelliferyl-β-D-glucuronide (MUG)
50mg
pH6.9±0.2 at 25°C
Preparation
Weigh 37g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until
completely dissolved, sterilize at 115℃ for 20min, cool to room temperature and set aside.
Quality Control
The following quality control strains were inoculated and cultured at 44.5℃±0.5℃ for 24h. The results are as follows:
Quality control strains
Growth
Escherichia coli ATCC25922
Blue-white fluorescence is produced under 366nm UV light
Salmonella typhimurium ATCC14028
No fluorescence under 366nm UV light
Enterococcus faecalis ATCC29212
Clear, no fluorescence
Storage and Shelf Life
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method. Principle and Interpretation MUG permits the rapid detec……
