Primer and probe mix (150 reactions) Reverse Transcription, target specific primers (RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control – Read through VIC channel* Endogenous control (150 tests) RNAse/DNAse free water *alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
Influenza A (H1N1) TaqMan RT-PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for H1N1
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Influenza is caused by three immunologic types of RNA viruses (A, B and C) within the Orthomyxoviridae family. Seasonal influenza is typically caused by three major subtypes of hemaglutinin (H1, H2 and H3) and two subtypes of neuraminidase (N1 and N2). A novel sub-type of influenza A virus called pandemic H1N1 2009 virus was identified in Mexico and reported by the CDC and WHO in April, 2009 (Novel swine-origin influenza A (H1N1) virus investigation team, 2009; CDC, 2009; and Fraser et al., 2009). H1N1 2009 is a novel sub-type virus that transmits easily between humans with 21 countries reporting cases within a month of initial identification (CDC, 2009-b). It is essential that public health laboratories around the world undertake detailed surveillance to monitor the spread and impact of pandemic H1N1 2009 virus as well as try to predict future changes in virulence (Fraser et al., 2009). Methods for the rapid diagnosis, case identification and tracking of this novel pathogen in the human population are therefore required to develop appropriate management strategies to mitigate morbidity and mortality.
H1N1 TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
H1N1 TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection. HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
Details
Specifications
Features
Specifications
Main Functions
Isolation both Circulating DNA/RNA (include miRNA) from 1-5 ml serum and plasma
Applications
qPCR / RT-PCR, liquid or solid-phasechip analysis, hybridization and SNP detection
Purification method
Midi spin column
Purification technology
Silica technology, DNA filtration technology
Process method
Manual (centrifugation or vacuum)
Sample type
serum, plasma, and other cell-free liquid samples
Sample amount
1-5 ml
Elution volume
≥20μl
Time per run
≤100 minutes
Liquid carrying volume per column
4 ml
Binding yield of column
1 mg
Principle
This kit is based on silica gel column technology. Serum or other liquid samples are lysed and digested in buffer CFL. After adding buffer CFP, the protein is removed by centrifugation to obtain the supernatant. Isopropanol is added to precipitate the total nucleic acid and transferred to the column for filtration. DNA / RNA is adsorbed on the membrane of the column, while the protein is not adsorbed and removed with the filtrate.The column is washed with buffer MGW1 to remove protein and other impurities, and then washed with buffer RW2 to remove salt. Finally, DNA / RNA is eluted by low salt buffer. The eluted DNA / RNA can be directly used for quantitative PCR/ RT-PCR, liquid or solid-phase chip analysis, hybridization and SNP detection.
Advantages
High yield – most optimized process to obtain maximum free RNA and small RNA
High concentration – low elution volume (>20μl) to ensure nucleic acid concentration
High purity – low alcohol combination, completely remove inhibitor and protein pollution
High recovery – silica gel column technology can recover nucleic acid molecules at the level of PG
Large volume – 1-2ml serum and plasma samples can be processed at one time
Kit Contents
Contents
R431602
D431603
Purification Times
50 Preps
250 Preps
HiPure RNA Micro Columns
50
5 x 50
HiPure Viral Midi Columns
50
5 x 50
15 ml Collection Tubes
50
5 x 50
2ml Collection Tubes
50
5 x 50
Buffer CFL
150 ml
2 x 375 ml
Buffer CFP
30 ml
150 ml
Buffer MGW1*
100 ml
2 x 250 ml
Buffer RW2*
2 x 50 ml
5 x 100 ml
RNase Free Water
10 ml
50 ml
Storage and Stability
The kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This kit provides a simple and fast solution for the extraction of circulating nuclear acid from serum, plasma, and other cell-free liquid samples. Circulating nucleic acid refers to the free extracellular nucleic acid produced by cell apoptosis, of which fragments are generally below 1KB. The kit is based on silica gel column purification technology, which is no need for toxic phenol chloroform extraction and time-consuming alcohol precipitation during the extraction. The obtained Circulating Nucleic Acid can be directly used for quantitative PCR, liquid or solid phase chip analysis, hybridization, and SNP detection. HiPure Circulating DNA/RNA Kit adopts a unique solution system and multiple layers of filter membranes with different pore sizes, which can efficiently process large volumes of serum and plasma samples and capture extremely small amounts of free nucleic acids.
