Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
022039P1 Mac Conkey Broth
Product Info
Document
Product Info
Introduction
Usages: For cultivation of Escherichia coli.
Principle: Peptone provide nitrogen, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium; lactose into fermentable sugars.
Formulation（per liter）:
Pancreatic Digest of Gelatin
20 g
Lactose Monohydrate
10g
Dehydrated Ox Bile
5g
Bromocresol Purple
10mg
final pH
7.3±0.2
How to use： 1.Suspend 35g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min. 2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Human Enterovirus (HEV) TaqMan RT-PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for HEV
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
The Enteroviruses are a genus of the family Picornaviridae, and are a large and diverse group of small RNA viruses characterized by a ssRNA molecule of positive sense. To date, over 70 serotypes of Enterovirus have been isolated from man. The human enteroviruses include poliovirus, Coxsackie A and Coxsackie B viruses, enterocytopathic human orphan (ECHO) viruses and other enteroviruses. Enteroviruses infect millions of people worldwide each year, and the infection can result in a wide variety of symptoms depending on the serotype of the enterovirus. Symptoms of infection may include, but are not limited to, mild respiratory illness (common cold), hand, foot and mouth disease, Rubelliform rashes, acute hemorrhagic conjunctivitis, aseptic meningitis, myocarditis, and neonatal infections.
HEV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
HEV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
