Attogene’s Cadmium Lateral Flow Kit can be used to detect Cadmium in water samples at 5 ppb in the field.
Format: 10 test cassettes
Run Time: 15-30 minutes
Cadmium contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Cadmium accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases. Long-term health consequences of drinking cadmium-contaminated food and water include numerous negative health effects. Attogene’s Cadmium Lateral Flow test gives results conforming of 5ppb or greater. Using the supplied pipette, simply fill the vial with your water sample, place the water into the sample port and wait 15-30 minutes.
Other Products
D3113 HiPure Tissue&Blood DNA Midi Kit
Product Info
Document
Product Info
Introduction
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern Blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 2ml blood and 200mg tissue using Midi column
Applications
PCR, southern bolt and virus detection, etc
Purification method
Midi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount
0.2-2 ml
Elution volume
≥300μl
Time per run
≤80 minutes
Liquid carrying volume per column
4ml
Binding yield of column
1mg
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast – without separation of leukocytes, organic extraction or ethanol precipitation
Simple – all nucleic acids can be obtained by direct digestion
Wide applicability – handle a variety of liquid samples
Kit Contents
Contents
D311302
D311303
Purification Times
20
100
HiPure gDNA Midi Columns
20
100
15ml Collection Tubes
40
200
Buffer ATL
50 ml
250 ml
Buffer AL
50 ml
250 ml
Buffer GW1*
22 ml
110 ml
Buffer GW2*
12 ml
50 ml
RNase A
20 mg
90 mg
Proteinase K
100 mg
440 mg
Protease Dissolve Buffer
10 ml
30 ml
Buffer AE
20 ml
120 ml
Storage and Stability
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column
Applications
RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Cells: ≤1 x 107 Animal tissue: 1-20 mgPlant leaves: 50-150 mg Yeast cells: 5 x 106
Yield
2-100μg
Elution volume
≥50μl
Time per run
≤15 minutes（1-24 samples）
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – several samples can be extracted in 20 minutes by column method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Kit Contents
Contents
R401102
R401103
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
50
250
RTL Lysis Buffer
50 ml
200 ml
RNA Binding Buffer
15 ml
75 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Document
The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Lyophilized format designed for room temperature shipping
Available in TaqMan format for analysis
Norgen’s Vibrio cholerae TaqMan PCR Lyophilized Kit is designed for the detection of Vibrio cholerae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s Vibrio cholerae TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Vibrio cholerae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
All kit components should be stored at -20°C upon arrival.
Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
