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Campylobacter jejuni TaqMan PCR Detection Kits

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Detail

Overview

  • Detection kits for Campylobacter jejuni
  • Available in TaqMan format for analysis.

Campylobacter jejuni is a curved, rod-shaped and microaerophillic gram negative bacterium. It is one of the most common causal agents of gastroenteritis with diarrhea as the main symptom. While infection of C. jejuni is seldom life-threatening, it is considered one of the most common food-borne bacteria with over 2 million people infected per year in US alone. Infection of C. jejuni usually results from consumption of poorly prepared food including undercooked meat (particularly poultry), untreated water or raw unpasteurized milk. Traditional identification of C. jejuni involves culturing, however the microaerophilic characteristic of this bacterium make the enrichment process laborious and costly.

Campylobacter jejuni TaqMan PCR Kit, 100 reactions

  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents

Campylobacter jejuni TaqMan PCR Probe/Primer Set and Controls, 100 reactions

  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

Figure 1 / 3

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Campylobacter jejuni TaqMan PCR Kit Figure 1
Campylobacter jejuni TaqMan PCR Kit Figure 1
Campylobacter jejuni TaqMan PCR Kit Figure 2
Campylobacter jejuni TaqMan PCR Kit Figure 3

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

ComponentCat. TM36150 (100 preps)Cat. TM36110 (100 preps)
MDx TaqMan 2X PCR Master Mix2 x 700 μL
Campylobacter jejuni Primer & Probe Mix280 μL280 μL
Campylobacter jejuni Positive Control150 μL150 μL
Nuclease-Free Water (Negative Control)1.25 mL1.25 mL
Product Insert11

Other Products

NGS Library Quantification Standards With PCR Primers (Ion Torrent Platform)

The NGS Library Quantification Standards with PCR Primers (for Ion Torrent platform) were developed for quantifying the library concentration for ion torrent sequencing platform. Quantification of the library of the fully ligated libraries is important for the quality of the sequencing outcome. Optimal library concentration can increase sequencing yield. Poor library concentration results in bad emPCR, which can lead to low sequencing capacity.

QPCR is the best method for library quantification. Our reagent only amplifies library molecules that will be used for subsequent emPCR, and is optimized for amplification of various samples. Our reagent is compatible with commercial SYBR Green based QPCR reagents. Quantification of library concentration is achieved by comparison with a standard curve generated from DNA Standards.

The kit comprises DNA Standards (six 10-fold dilutions) and a primer mix.

NGS Library Quantification Standards (Ion Torrent)

NGS Library Quantification Standards with PCR Primers (Ion Torrent platform): real time quantitative PCR curve of the standards.

Zika Virus (ZIKV) TaqMan RT-PCR Detection Kits

Overview

  • Detection kits for ZIKV
  • CE-IVD marked version available for in vitro diagnostic use
  • Available in TaqMan format for analysis

The Zika Virus (ZIKV) is an emerging mosquito-borne virus that was first identified in Uganda in 1947 in Rhesus monkeys through monitoring of sylvatic yellow fever. During large outbreaks in French Polynesia and Brazil, national health authorities reported potential neurological and auto-immune complications of ZIKV disease. Agencies investigating the Zika outbreaks are finding an increasing body of evidence about the link between ZIKV and microcephaly. Infection with ZIKV may be suspected based on symptoms and recent history (e.g. residence or travel to an area where ZIKV is known to be present). Zika virus diagnosis can only be confirmed by laboratory testing for the presence of ZIKV RNA in the blood or other body fluids, such as urine or saliva.

ZIKV TaqMan RT-PCR Kit, 100 reactions

  • Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
  • Specific Primer and Probe mix for the pathogen/virus/viroid of interest
  • Primer and Probe mix
  • Positive and negative control to confirm the integrity of the kit reagents

ZIKV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

  • Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
  • Nuclease-free water
  • Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

Figure 1 / 4

Previous

ZIKV TaqMan PCR Kit Figure 1
ZIKV TaqMan PCR Kit Figure 1
ZIKV TaqMan PCR Kit Figure 2
ZIKV TaqMan PCR Kit Figure 3
ZIKV TaqMan PCR Kit Figure 4

Next

Click for expanded view

Storage Conditions

All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

ComponentCat. TM62250 (100 preps)Cat. TM62210 (100 preps)
MDx TaqMan 2X PCR Master Mix2 x 700 μL
ZIKV Primer & Probe Mix280 μL280 μL
ZIKV Positive Control150 μL150 μL
Nuclease-Free Water (Negative Control)1.25 mL1.25 mL
Product Insert11

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

Product Details

Application:

DNA Nucleic Acid

Format:

NFO Kit

Kit Components:

Reagents,Buffer,Enzymes

Manufacturer:

Amp-future Bio

Product Name:

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

Reaction Time:

5-20mins

Reaction Volume:

50μL

Sensitivity:

500-1000copies/ml

Shelf Life:

14 Months

Specificity:

High

Storage Conditions:

-20℃

Suitability:

Universal

High Light:

Isothermal DNA Amplification Kit

DNA Amplification Kit NFO

20mins nucleic acid amplification

Payment & Shipping Terms

Minimum Order Quantity

48T

Price

USD$3.8/T

Packaging Details

16T/Bag,48T/Box

Delivery Time

6 working days

Payment Terms

T/T, MoneyGram

Supply Ability

100000T/Month

Product Description

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type) 0

【Principle Overview】

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.

【Primer design】

It is recommended to use primers with a length of 30-35 bp.Too short primers will affect the amplification speed and detection sensitivity;the 5′ end of the reverse primer is labeled with a modification group (commonly used biotin).

Primers are designed to avoid the formation of secondary structures that affect amplification; the length of the amplicon is recommended to be 150-500bp.

【Colloidal gold probe design】

In the middle of the forward and reverse primer,design a sequence of 46-52nt in length that is complementary to the target fragment; modify an antigen marker (typical FAM) at the 5′ end;mark a dSpacer (tetrahydrofuran, THF) in the middle of the 5′ end and the 3′ end ), as the recognition site of nfo;the 3′ end is labeled with a modification group, such as amine group, phosphate group or C3-Spacer, etc.

【Features】

This kit has the advantages of high sensitivity, strong specificity,and short reaction time (only 15 minutes),and the reaction components are in dry powder state,which is easy to operate and easy to store.

This reagent has low equipment requirement,and the reaction operation can be carried out in metal baths, water baths, etc.,and there is no need to purchase expensive exclusive equipment such as PCR amplifiers.

DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type) 2
CompositionContent
A buffer1.6mL×1Tube
B buffer150μL×1Tube
Positive control template-II100μL×1Tube
Positive control probe and primer mix-II70μL×1Tube
Reagent48 T
Guide Manual1 Copy

Kit storage

1. Storage conditions: storage temperature ≤ -20℃constant temperature environment, keep away from light and avoid heavy pressure;

2. Product validity period: 14 months;

3. See the outer packaging for the production date.