Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.
Detail
General information
Champion™ Competent Cells are chemically competent cells, which were prepared by SMOBIO to make E. coli perform excellent transformation efficiency. Standard transformation protocol is recommended for large plasmids or non-ampicillin selection. Time-saving transformation protocol is recommended for simple and rapid transformation. Champion™ Competent Cells are one of the fastest and simplest ready-to-use competent cell products in the world.
Kit contents
Champion™ Competent Cells
pUC19 Control Plasmid (5 μl, 10-4 μg/μl)
Champion™ Transformation Protocol Card
Shipping condition
Throughout the shipping process, the temperature is maintained under -70°C.
Storage and expiration
Champion™ Competent Cells must be stored between -70°C to -80°C. Subsequent freeze-thaw cycles will reduce transformation efficiency. If high efficiency is required for the experiment, do not use aliquots that have gone through several freeze-thaw cycles. The efficiency of Champion™ Competent Cells lasts for 1 year with proper storage.
Other Products
Ammonia Assay Kit (Liquid Ready™)
Product Info
Document
Product Info
K-AMIARLQ
SKU: 700007403
50 assays (manual) / 500 assays (auto-analyzer)
Content:
50 assays (manual) / 500 assays (auto-analyzer)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Analyte:
Ammonia, Nitrogen, YAN
Assay Format:
Spectrophotometer, Auto-analyzer
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Decrease
Linear Range:
0.4 to 8 µg of ammonia per assay
Limit of Detection:
1 mg/L
Limit of Quantification:
3 mg/L
Reproducibility (%):
< 5%
Reaction Time (min):
~ 5 min
The Ammonia (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of ammonia in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
“Ready to use” liquid stable formulation – no reconstitution needed
Rapid reaction (~ 5 min)
PVP incorporated to prevent tannin inhibition
Standard included
Suitable for manual and auto-analyzer formats
Quick Reference Guide available
Mega-Calc™ software tool is available for hassle-free raw data processing
Document
The Ammonia (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of ammonia in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
DBCO-PEG24-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
DBCO-PEG24-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA from FFPE tissue
Applications
RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc.
Purification method
Polydisperse silicon based magnetic beads (DNA)Monodisperse carbonyl magnetic beads (RNA)
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor and pipetting workstation
Sample type
FFPE slice, FFPE puncture sample, embedded tissue
Sample amount
No more than six 10 µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area
Yield
DNA: 1 – 10 μg, RNA: 1 – 25 μg
Principle
The sample is lysed and digested under the action of lysate and protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, the supernatant contain RNA was collected and bind to MagBind Particles. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Elution Buffer.
Advantages
High quality – high purity total RNA / DNA can be directly used in various downstream applications
Safe – no phenol chloroform extraction required
Simultaneous extraction – simultaneously isolate DNA and RNA from one sample
Post digestion sorting – higher DNA and RNA yields
High RNA yield – monodisperse carbonyl adsorption
Kit Contents
Contents
R632701
R632702
R632703
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagBind Particles
1.1 ml
2.5 ml
11 ml
MagPure Particles N
1.1 ml
2.5 ml
11 ml
Proteinase K
24 mg
48 mg
220 mg
Protease Dissolve Buffer
3 ml
10 ml
15 ml
Buffer DPS
50 ml
100 ml
2 x 250 ml
Buffer ATL
20 ml
30 ml
120 ml
Buffer BST1
20 ml
40 ml
200 ml
Buffer BXW1*
44 ml
110 ml
3 x 110 ml
RNase Free Water
15 ml
30 ml
120 ml
Storage and Stability
Proteinase K, MagPure Particles N and MagBind Particles should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stablefor at least 18 months under these conditions.
Experiment Data
Document
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and pyrosequencing.