Overview

• Detection kit for Toxoplasma gondii
• Available in TaqMan format for analysis

Toxoplasma gondii is a single-celled organism which causes Toxoplasmosis, the most common parasitic infection worldwide affecting animals as well as humans. Cats are the only animal in which sexual reproduction of the organism occurs, and for this reason cats are the only domestic animal which has the potential to shed T. gondii eggs. When disease does occur, it may develop following primary infection due to an inadequate immune response to stop the invasive tachyzoites or as a result of a reactivated infection due to a compromised immune system. The most common symptoms of the disease are anorexia, weight loss, lethargy, dyspnea, ocular symptoms and pyrexia. As toxoplasmosis can be transmitted to humans it represents a serious health risk for people living in close contact with infected animals. Infection is especially dangerous for people with suppressed immune systems and cancer patients as well as pregnant women.

Toxoplasma gondii TaqMan PCR Kit,
100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Toxoplasma gondii TaqMan PCR Probe/Primer Set and Controls,
100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions

All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	TaqMan Probe Cat. TM44750 (100 preps)	TaqMan Probe Cat. TM44710 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 µL	–
Toxoplasma gondii Primer Probe Mix	280 µL	280 µL
Toxoplasma gondii Positive Control	150 µL	150 µL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Introduction

This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.

Details

Specifications

Features	Specifications
Main Functions	Co-isolation DNA and RNA from skin, muscle, connective tissue, fibrous tissue sample
Applications	PCR and southern blot, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Cultured cells and tissue samples
Sample amount	Tissue: < 20mg

Principle

The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.

Advantages

• High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
• Fast – column method can complete the extraction of several samples in 30 minutes
• Safe – no phenol chloroform extraction required
• Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

Kit Contents

Contents	R511402	R511403
Purification Times	50 Preps	250 Preps
HiPure DNA Mini Columns	50	250
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	100	2 x 250
Buffer RL	30 ml	150 ml
RNA Digestion Buffer	15 ml	80 ml
Buffer DW1	30 ml	150 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	20 ml	2 x 50  ml
RNase Free Water	10 ml	30 ml
Buffer AE	10 ml	50 ml

Storage and Stability

HiPure DNA/RNA Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.

This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.

t-Boc-N-Amido-PEG9-propargyl is an alkyne linker that can be used in Click Chemistry reactions with azides to yield a stable triazole linkage; copper is required for catalyzation. Under mild acidic conditions, the Boc group can be removed to form a free amine. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

t-Boc-N-Amido-PEG9-propargyl is an alkyne linker that can be used in Click Chemistry reactions with azides to yield a stable triazole linkage; copper is required for catalyzation. Under mild acidic conditions, the Boc group can be removed to form a free amine. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.