[CD1011] SMOChem™ Deoxynucleotide (dNTP) Mix, 10 mM each (40 mM total), 200 µl x 5
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The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
Detail
Description
The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
Features
Ideal for PCR amplification and cDNA synthesis
Premixed solution
Nuclease and ribonuclease free
Applications
PCR amplification of DNA fragments
DNA fill-in reaction
DNA sequencing
Reverse transcription
One-step RT-PCR
Storage
-20°C for 36 months
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Product Info
Document
Product Info
Introduction
Usage: For cultivating, enumerating and preserving non-fastidious bacteria. (ISO)
TCO-PEG12-DBCO is a long chain PEGreagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine under mild condition. DBCO is very reactive toward Azide through click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
TCO-PEG12-DBCO is a long chain PEGreagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine under mild condition. DBCO is very reactive toward Azide through click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles
Applications
RT-PCR, cDNA synthesis, second generation sequencing
Purification method
Polydisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Adaptive instrument
Nucleic acid extractor, pipetting workstation
Sample type
Serum, plasma, acellular samples
Sample amount
300μl
Principle
This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
Safe – no phenol chloroform extraction and no use of Trizol reagent
Fast – several samples can be extracted in 60 minutes by column method
Kit Contents
Contents
R662801
R662802
R662803
Purification Times
48 Preps
96 Preps
5 x 96 Preps
MagPure Particles N
1.7 ml
3.5 ml
17 ml
Buffer CFL
6 ml
12 ml
60 ml
Buffer CPL
1.8 ml
3.5 ml
20 ml
Buffer MGW1*
30 ml
60 ml
250 ml
Buffer MW2*
12 ml
25 ml
100 ml
RNase Free Water
10 ml
20 ml
60 ml
Storage and Stability
MagPure Particle N should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.