[CD1011] SMOChem™ Deoxynucleotide (dNTP) Mix, 10 mM each (40 mM total), 200 µl x 5
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The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
Detail
Description
The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.
Features
Ideal for PCR amplification and cDNA synthesis
Premixed solution
Nuclease and ribonuclease free
Applications
PCR amplification of DNA fragments
DNA fill-in reaction
DNA sequencing
Reverse transcription
One-step RT-PCR
Storage
-20°C for 36 months
Other Products
myo-Inositol Assay Kit
Product Info
Document
Product Info
K-INOSL
SKU: 700004304
50 assays per kit
Content:
50 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
myo-Inositol
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
492
Signal Response:
Increase
Linear Range:
2 to 35 µg of myo-inositol per assay
Limit of Detection:
0.8 mg/L
Reaction Time (min):
~ 30 min
Application examples:
Animal feeds, food and other materials.
Method recognition:
Novel method
The myo-Inositol Assay Kit is a reliable and accurate enzymatic UV-method for the specific measurement and analysis of myo-inositol in animal feeds, foods and various other materials.
Phytic acid content of samples with very low levels of free myo-inositol can also be determined using K-INOSL. This can be achieved by measuring the amount of myo-inositol released after de-phosphorylation of phytic acid with the enzymes phytase and alkaline phosphatase, as used with the Megazyme Phytic Acid/Total Phosphorus Assay Kit (K-PHYT).
Not suitable for the determination of myo-inositol in baby formula.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
All reagents stable for > 2 years after preparation
Only enzymatic kit available
Rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Document
The myo-Inositol Assay Kit is a reliable and accurate enzymatic UV-method for the specific measurement and analysis of myo-inositol in animal feeds, foods and various other materials.
For cultivating and enriching nofastidious bacteria ,and for evaluating the efficacy of disinfection.
Formula (per liter)
Peptone—————10.0g Beef Extract———–3.0g Sodium Chloride—–5.0g Final pH———–7.4 ± 0.2
How to use
1.Suspend 18g in 1L of distilled water , stirring heated to boiling to completely dissolve ,autoclave at 121ºC for 15 minutes. 2.Diluted and treated samples.
Storage
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
