Description
Ultrapure dCTP supplied as sodium salt in purified water (pH 8.5).
Features
- Ideal for PCR amplification and cDNA synthesis
- Nuclease and ribonuclease free
Applications
- PCR
- RT-PCR
Storage
-20°C for 36 months
Ultrapure dCTP supplied as sodium salt in purified water (pH 8.5).
Description
Ultrapure dCTP supplied as sodium salt in purified water (pH 8.5).
Features
Applications
Storage
-20°C for 36 months
The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).
The kit uses linear dsDNA libraries (MGI platform) as input and enriches the circularized single-stranded DNA libraries. The circularization kit has a higher library circularization efficiency (25%) as compared to other vendors (7-15%).
Kit features
The NGS Library Circularization Kit (MGI Platform) was developed for preparation of single-stranded circular DNA libraries for next generation sequencing (MGI platform).
Product Details
Application:
DNA Nucleic Acid Amplification
Brand:
Amp-future Bio
Components:
Polymerase, Buffer
Name:
DNA Isothermal Rapid Amplification Kit(Basic)
Reaction Time:
5-20mins
Sensitivity:
High
Shelf Life:
14 Months
Size:
50 Reactions
Storage:
-20°C
Type:
Basic
High Light:
,
,
Payment & Shipping Terms
Minimum Order Quantity
48T
Price
USD$3.8/T
Packaging Details
16T/bags,48T/Box
Delivery Time
14days
Payment Terms
T/T, , MoneyGram
Supply Ability
100000T/Months
Product Description
Reagent component ( WLB8201KIT ,16T/bags,48T/Box ) | |||
Component | Specification | Quantity | Function |
A buffer | 1.6ml | 1 Tube | Buffer system mainly for stabilizing protein/enzyme and performance |
B buffer | 0.15ml | 1 Tube | Mainly activated systems such as magnesium ions |
Positive control template | 0.1ml | 1 Tube | Mainly the positive plasmid template is used to test the effectiveness of the kit |
Positive control primer mix | 0.06ml | 1 Tube | Mainly the primer combination of the positive control template |
Reagent Guide Manua | 16T/bags,48T/Box | 3 bags | Reagent technology of protein/enzyme system: freeze-dried powder, freeze-dried microspheres |
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39ºC~42ºC), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension.It is suitable for laboratory-level DNA amplification and DNA amplification for other detection purposes.
Salmonella spp. are members of the family Enterobacteriaceae. They are Gram-negative, facultatively anaerobic, flagellated, rod-shaped organisms. They are approximately 0.7 to 1.5 µm in diameter and 2 to 5 µm in length and responsible for a large number of cases of foodborne illness throughout the world. Salmonella have circular DNA genomes with a mean length of approximately 4530 kb, although this can vary by up 1000 kb. Salmonella classification is extremely complex, however, the genus is divided into two species: S. enterica and S.bongori. S. enterica is then itself divided into 6 biochemically distinct subspecies and the Salmonella genus is further classified into serovars (serotypes) based on the lipopolysaccharide (O), flagella protein (H), and sometimes the capsular (VI) antigens. There are more than 2500 known serovars and within a serovar there may be strains that differ in virulence.
Salmonella are mainly transmitted by the faecal-oral route. They are carried asymptomatically in the intestines or gall bladder of many animals, being continuously or intermittently shed in the faeces. Humans can become infected if they do not wash their hands after contact with infected animals or animal faeces. In such instances the bacteria adhere to and enter the cells of the intestinal epithelium. The toxins produced by the bacteria can damage and kill the cells that line the intestines, which results in intestinal fluid loss. The bacteria can survive for weeks in a dry environment and far longer in water thus they are frequently present in polluted waters. Salmonella can also be carried latently in the mesenteric lymph nodes or tonsils; these bacteria are not shed, but can become reactivated after stress or immunosuppression. In addition, fomites and vectors can spread Salmonella and vertical transmission occurs in birds, with contamination of the vitalize membrane, albumen and possibly the yolk of eggs. Salmonella spp. can also be transmitted in utero in mammals.
There are two different disease conditions that are distinct to salmonellosis; gastroenteritis and enteric typhoid fever. The gastroenteritis is a nonsystemic infection of the intestinal tract and regional lymph nodes that gives rise to headache, muscle aches, diarrhoea, vomiting, abdominal cramping, chills, fever, nausea and dehydration. In contrast, the enteric typhoid fever is a systemic disease in which the microorganism replicates within the cells of the reticuloendothelial system. The symptoms usually appear 6 to 72 hours after ingesting contaminated food although individuals can be infected with the bacteria without having symptoms. Those with and without symptoms shed the bacteria in their stool and it is important that personal hygiene be maintained at all times.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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