
Description
Ultrapure dCTP supplied as sodium salt in purified water (pH 8.5).
Features
- Ideal for PCR amplification and cDNA synthesis
- Nuclease and ribonuclease free
Applications
- PCR
- RT-PCR
Storage
-20°C for 36 months
Ultrapure dCTP supplied as sodium salt in purified water (pH 8.5).
Description
Ultrapure dCTP supplied as sodium salt in purified water (pH 8.5).
Features
Applications
Storage
-20°C for 36 months
This kit is designed for the rapid spin column preparation of genomic DNA from 2 x 109 viable bacterial cells (between 0.5 and 1.0 mL of culture). This kit can be used for both Gram-negative and Gram-positive bacteria including Escherichia coli and Bacillus cereus. Purified genomic DNA is of an excellent quality and yield, and is fully compatible with restriction enzyme digestions, sequencing, PCR, qPCR and more. Also available in 96-well format for high throughput applications.
Figure 1 / 2
Click for expanded view
Kit Specifications (Spin Columns) | |
Input | 2 x 109 bacterial cells |
Column Binding Capacity | 25 µg |
Average Yield* | Up to 20 µg |
Time to Complete 10 Purifications | 1 hour |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
Component | Cat. 17900 (50 preps) | Cat. 17950 (192 preps) |
---|---|---|
Resuspension Solution A | 20 mL | 60 mL |
Lysis Buffer P | 18 mL | 60 mL |
Solution BX | 28 mL | 110 mL |
Wash Solution A | 18 mL | 2 x 38 mL |
Elution Buffer B | 30 mL | 2 x 30 mL |
Proteinase K | 12 mg | 50 mg |
Spin Columns | 50 | – |
96-Well Plate | – | 2 |
Adhesive Tape | – | 4 |
Collection Plate | – | 2 |
Collection Tubes | 50 | – |
Elution Tubes (1.7 mL) | 50 | – |
Elution Plate | – | 2 |
Product Insert | 1 | 1 |
Usages:
For cultivation of Escherichia coli.
Principle:
Peptone provide nitrogen, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium; lactose into fermentable sugars.
Formulation(per liter):
Pancreatic Digest of Gelatin | 20 g |
Lactose Monohydrate | 10g |
Dehydrated Ox Bile | 5g |
Bromocresol Purple | 10mg |
final pH | 7.3±0.2 |
How to use:
1.Suspend 35g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 ℃ autoclave for 15min.
2.Diluted and treated samples.
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250g
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map