Cell Culture Dish 35mm

Overview

• Extract high quality & quantity total RNA including miRNA
• No phenol step required; isolate all RNA in one fraction
• Bind & elute all RNA irrespective of size or GC content, without bias
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Isolate from a wide variety of specimens
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Available in a variety of formats to suit your needs
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A₂₆₀/A₂₈₀ suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)

This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Isolate RNA after Purifying EVs and Exosomes

Ultracentrifugation, Exoquick, Filtration

Cat. #	Name	Elution Volume	Plasma/Serum	Urine	Cell-Culture Media
55000	Plasma/Serum RNA Purification Mini Kit	10 – 25 µL	50 µL – 1 mL	250 µL – 1 mL	5 – 10 mL
35300	Total RNA Purification Micro Kit	20 – 50 µL	1 – 4 mL	2 – 10 mL	10 – 20 mL
17200	Total RNA Purification Kit	50 – 100 µL	4 – 10 mL	11 – 30 mL	20 – 35 mL

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity	Up to 50 μg RNA
Maximum Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Animal Cells	3 x 106 cells
Animal Tissues	10 mg (for most tissues*)
Blood	100 μL
Plasma/Serum	200 μL
Bacteria	1 x 109 cells
Yeast	1 x 108 cells
Fungi	50 mg
Plant Tissues	50 mg
Time to Complete 10 Purifications	20 minutes
Average Yield
HeLa Cells (1 x 106 cells)	15 μg
E. coli (1 x 109 cells)	50 μg

* for isolating total RNA from larger amounts of tissue, please use Norgen’s Animal Tissue RNA Purification Kit (Cat# 25700)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

Component	Cat. 17200 (50 preps)	Cat. 37500 (100 preps)	Cat. 17250 (250 preps)	Cat. 17270 (500 preps)	Cat. 24300 (192 preps)	Cat. 24370 (576 preps)	Cat. 24350 (192 preps)	Cat. 24380 (576 preps)
Buffer RL	40 mL	2 x 40 mL	175 mL	350 mL	2 x 40 mL	350 mL	2 x 40 mL	350 mL
Wash Solution A	38 mL	2 x 38 mL	148 mL	1 x 148 mL 1 x 74 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL	2 x 38 mL	1 x 74 mL 1 x 148 mL
Elution Solution A	6 mL	2 x 6 mL	30 mL	60 mL	2 x 20 mL	60 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	100	250	500	–	–	–	–
96-Well Isolation Plate	–	–	–	–	2	6	–	–
96-Well Isolation Plate (Deep Well)	–	–	–	–	–	–	2	6
Adhesive Tape	–	–	–	–	4	12	4	12
Collection Tubes	50	100	250	500	–	–
96-Well Collection Plate	–	–	–	–	2	6	–	–
96-Well Collection Plate (Deep Well)	–	–	–	–	–	–	2	6
Elution Tubes (1.7 mL)	50	100	250	500	–	–
96-Well Elution Plate	–	–	–	–	2	6	–	–
96-Well Elution Plate (Deep Well)	–	–	–	–	–	–	2	6
Product Insert	1	1	1	1	1	1	1	1

Description

As this is a 2 gene kit, we recommend purchase of 2 of the accompanying RT-qPCR master mix reagent: oasig Lyophilised OneStep RT-qPCR Master Mix 150 reactions.

Norovirus is known to cause acute gastroenteritis. It is a small (27-38 nm), round, nonenveloped RNA virus belonging to the Caliciviridae family and is responsible for over 80% of non-bacterial outbreaks of gastroenteritis in the world. It affects individuals of all ages, with a distinct seasonal link to winter. It has a genome of 7.6 kb that is positive sense and has a single stranded linear confirmation. It encodes a major structural protein (VP1) of about 58 to 60 kDa and a minor capsid protein (VP2). Transmission occurs predominantly through ingestion of contaminated water, food and airborne transmission, as well as contact with contaminated surfaces. The ease with which norovirus is transmitted and the low infectious dose required to establish an infection results in extensive outbreaks in numerous environments, such as hospitals, hotels and schools. There is no antiviral drug available to treat this infection and little is known about its pathogenicity. However, it has been observed that the virus can be taken up by enterocytes where translation of viral nonstructural proteins can occur; it damages and alters intestinal microvilli, leaving them blunt and broadened, thus inhibiting absorption; it causes crypt cell hyperplasia and also leads to apoptosis of enterocyctes. An incubation period of 24-48 hours is usual. Infection is characterized by the acute onset of nausea, vomiting, abdominal cramps, aching limbs, raised temperature and diarrhoea that generally last for about 48 hours. However, more severe and prolonged infection may be observed in children and the elderly. There are five recognized norovirus genogroups, of which three (GI, GII, and GIV) are known to affect humans and, since 2002, variants of the GII.4 genotype have been the most common cause of norovirus outbreaks. There have been 31 different genotypes identified within the genogroups, with a wide degree of genetic variability present even within each genotype.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings

Description 

The DM2360 FluoroBand™ 100 bp+3K Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2360 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank. 

Features

• Sharp bands
• Quick reference— enhanced bands 
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage over 6 months
• Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye

Source 

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range 

100 ~ 3,000 bp 

Concentration 

56 µg/ 500 µl

Recommended loading volume 

5 µl/ well

Storage

Protected from light 
Room temperature for 6 months
4°C for 12 months 
-20°C for 24 months 

The DM2360 FluoroBand™ 100 bp+3K Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM2360 is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.