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Cell Culture Flask T75

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Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.

Volume: 25mL75mL175mL225mL

Detail

Cell Culture Flask T75

Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.

Volume: 25mL75mL175mL225mL

PRODUCT FEATURES

  • The product is made of medical grade USP CLASS VI polymer polystyrene
  • The product is made under a 100,00- class dust-free manufacturing site
  • Two kinds of product line up are providing.

For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.

For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.

  • Large mouthed design makes easy operation of pipet or cell scraper. The surface of flask is uniform and smooth, hence the clear view can be obtained when microscopic observation.
  • The hydrophobic filter cap can prevent invasion of fungi and bacteria without water absorb.
  • Gamma radiation sterilization
  • Non-Pyrogenic, DNase/Rnase free.

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Methyltetrazine-PEG7-DBCO

Methyltetrazine-PEG7-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DNA Isothermal Amplification Kit NFO With 500-1000 Copies/UL Detection Limit 48 Tests / Box

Product Description

Amp-future DNA Isothermal Amplification Kit NFO with 500-1000copies/µL Detection Limit, 48 Tests/box, -20°C Storage

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Technical Parameters:

ParametersDetails
Product NameDNA Isothermal Amplification Kit NFO
ManufacturerAmp-future
Storage Temperature-20°C
Kit ComponentsEnzymes, Buffers ,Reagents
Packaging48 Tests/box
Detection Limit500-1000copies/µL
ShippingICE
Test Time5-20mins

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

PCR Purification Kit

Overview

  • Purify amplified DNA ranging from 100 bp -15,000 bp in size
  • Fast and efficient spin column format 
  • Available in a 50 prep size and a 250 prep size
  • Also available in 96 well format
     

This kit enables the rapid purification of amplified DNA products from PCR mixes. It is able to effectively remove PCR by-products including primers, dimers, enzymes, unincorporated nucleotides and mineral oil from the desired PCR product. The purified PCR products are fully compatible with restriction enzyme digestion, ligation into vectors, labeling, sequencing and more. This kit can also be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions.

The kit is also available in a 96-well format for high-throughput PCR purification. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Details

Supporting Data

Figure 1 / 2

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Figure 1. High Yield Purification
Figure 1. High Yield Purification
Figure 2. Removal of Primer-Dimers

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Kit Specifications – 96-well
Binding Capacity Per Well15 μg
Maximum Loading Volume Per Well500 μL
Size of DNA Purified100 – 15,000 bp
Average DNA Recovery> 90%
Average Primer Reoval> 90%
Minimum Elution Volume50 μL
Time to Complete 96 Purifications20 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

ComponentCat. 14400 (50 preps)Cat. 45700 (250 preps)Cat. 24800 (192 preps)
Binding Buffer C30 mL5 x 30 mL3 x 30 mL
Wash Solution A12 mL2 x 20 mL2 x 38 mL
Elution Buffer B8 mL2 x 30 mL2 x 15 mL
Spin Columns50250
Collection Tubes50250
96-Well Plate2
Adhesive Tape4
96-Well Collection Plate2
Elution Tubes (1.7 mL)50250
96-Well Elution Plate2
Product Insert112