Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.
Volume: 25mL75mL175mL225mL
Detail
Cell Culture Flask T75
Cell Culture Flask surface is very smooth based on precise molding technology and it gives clear view when examined with microscope.
Volume: 25mL75mL175mL225mL
PRODUCT FEATURES
The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Large mouthed design makes easy operation of pipet or cell scraper. The surface of flask is uniform and smooth, hence the clear view can be obtained when microscopic observation.
The hydrophobic filter cap can prevent invasion of fungi and bacteria without water absorb.
Gamma radiation sterilization
Non-Pyrogenic, DNase/Rnase free.
Other Products
TGL20 Table Top High Speed Refrigerated Centrifuge
Product Info
Document
Product Info
TGL20 Technical Parameters
Max Speed
21000r/min
Max Volume
6×100ml
Timer
1~99h59min
Dimension
570×620×380mm
Speed Accuracy
±20r/min
Max RCF
30910×g
Noise
≤58dBA
Net Weight
84KG
Power supply
AC 220V, 50HZ 10A
Temperature Range
-20℃~40℃
Temperature Accuracy
±1℃
Matched Rotors for TGL20
Order no
Rotor
Max Speed(r/min)
Volume(ml)
Max RCF(*g)
G20-1
Fixed Rotor
16000
4×8PCR
15760
G20-2
Fixed Rotor
15000
6×8PCR
21420
G20-3
Fixed Rotor
16000
8×8PCR
17480
G20-4
Fixed Rotor
15000
12×8PCR
22930
G20-5
Fixed Rotor
21000
12×1.5/2ml
30910
G20-6
Fixed Rotor
15000
40×0.5ml
22920
G20-7
Fixed Rotor
17000
24×1.5/2ml
26460
G20-8
Fixed Rotor
13500
30×1.5/2ml
19340
G20-9
Fixed Rotor
13000
48×1.5/2ml
17930
G20-10
Fixed Rotor
16000
16×5ml
22020
G20-11
Fixed Rotor
16000
6×10ml
21500
G20-12
Fixed Rotor
15000
12×10ml
22680
G20-13
Fixed Rotor
16000
12×7ml
21380
G20-14
Fixed Rotor
13000
16×10ml
19490
G20-15
Fixed Rotor
10000
12×15ml
11840
G20-16
Fixed Rotor
13000
8×15ml(falcon)
17790
G20-17
Fixed Rotor
5000
24×15ml
3500
G20-18
Fixed Rotor
15000
8×20ml
22680
G20-19
Fixed Rotor
5000
30×15ml
3830
G20-20
Fixed Rotor
14000
6×30ml
19060
G20-21
Fixed Rotor
13000
6×50ml(falcon)
18840
G20-22
Fixed Rotor
13000
6×50ml(round)
18730
G20-23
Fixed Rotor
13000
4×85ml
18940
G20-24
Fixed Rotor
12000
6×70ml
15570
G20-25
Fixed Rotor
12000
4×100ml
14850
G20-26
Fixed Rotor
12000
6×100ml
16390
G20-27
Fixed Rotor
12000
24 pieces capillary vessel
15800
G20-28
Fixed Rotor
18000
30×0.5ml
26660
G20-29
Swing Rotor
13000
4×5ml
14960
G20-30
Vertical Rotor
16000
16×5ml
16540
G20-31
Vertical Rotor
14000
8×30ml
16450
G20-32
Microplate Rotor
4000
2×3×48(well)
2300
Document
Features:
1. Digital display indicates the speed,time,temperature and RCF.
2. Brushless DC motor in great torque, free maintenance,no powder pollution.quick in speed up and down.
3. There are many kinds of rotors for choice.
4. Automatically electric lid lock,super speed,over temperature protection and imbalance protection.
5. The centrifuge body is made of high-quality steel,safe and reliable.
West Nile Virus (WNV) TaqMan RT-PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for WNV
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level.
WNV TaqMan RT-PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
WNV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
Storage Conditions and Product Stability All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Gel images of different ranges of library size selection. Sheared human genomic DNA was used as input.
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Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.
There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.
Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.
The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.
Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.
NGS library size selection with dual clean-ups.
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Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.
NGS library size selection with single clean-up for >5 kb and >10 kb libraries.