Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Actin is part of the cytoskeletal system of all cell types. Smooth Muscle Actin is found in myofibroblasts and myoepithelium, but not in cardiac and skeletal muscles. Labelling of smooth muscle actin in concert with muscle specific actin staining can allow for differentiation between rhabdomyosarcoma and leiomyosarcoma, as Muscle specific actin is found in rhabdomyoblasts, while smooth muscle actin is found in leiomyosarcomas.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from insect tissue
Applications
PCR, southern bolt and virus detection, etc
Purification method
96 well DNA plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Insect tissue samples
Sample amount
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity DNA – can be used in sensitive downstream applications such as multiplex and quantitative pcr
Good repeatability – suitable for extracting high-yield DNA from insect tissue samples
Safe – without phenol chloroform extraction and alcohol precipitation
Kit Contents
Contents
D313901
D313902
Purification Times
1 x 96
4 x 96
HiPure DNA Plate
1
4
2.2 ml Collection Plate
1
4
1.6 ml Collection Plate
1
4
0.5ml Collection Plate
1
4
Seal Film
8
32
Buffer ITL
30 ml
120 ml
Buffer IL
30 ml
125 ml
Buffer GW1
44 ml
2 x 110 ml
Buffer GW2
50 ml
3 x 50 ml
Proteinase K
50 mg
200 mg
Protease Dissolve Buffer
6 ml
15 ml
Buffer AE
20 ml
60 ml
Storage and Stability
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
Document
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Methyltetrazine-PEG5-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Methyltetrazine-PEG5-DBCO is a TCO reactive reagent with a DBCO group and water-soluble PEG spacer. This reagent can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
