Checkit Go enables you to easily measure the volumes dispensed by a robot or multi-channel pipette, in under 10 seconds. These models measure up to 8 channels at a time and are offered in volume ranges covering 2μL to 50μL.
Please choose the model that matches your volume range:
Model Volume Range
5 μL Volumetric Verification Kit 2-5 μL
10 μL Volumetric Verification Kit 4-10 μL
20 μL Volumetric Verification Kit 8-20 μL
50 μL Volumetric Verification Kit 20-50 μL
Checkit Go enables you to easily measure the volumes dispensed by a robot or multi-channel pipette, in under 10 seconds. These models measure up to 8 channels at a time and are offered in volume ranges covering 2μL to 50μL.
Please choose the model that matches your volume range:
| Model | Volume Range |
|---|---|
| 5 μL Volumetric Verification Kit | 2-5 μL |
| 10 μL Volumetric Verification Kit | 4-10 μL |
| 20 μL Volumetric Verification Kit | 8-20 μL |
| 50 μL Volumetric Verification Kit | 20-50 μL |
Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products.
Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.
Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes:
Cat # 28113 – 100 reactions (sufficient for 100 reactions x 20 µL reaction volume)
Cat # 28114 – 200 reactions (sufficient for 200 reactions x 20 µL reaction volume)
Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)
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Reagents supplied – Cat # 28113
Storage Conditions and Product Stability
2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.
N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.
N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.
Permagen’s 0.2 mL PCR / 1.5 mL Microcentrifuge tube separation rack is designed for labs with both PCR strip and 1.5 mL separation protocols. On the top, hold up to two of either 8 or 12 strips, or up to 24 individual tubes. Flip over and hold up to twelve 1.5 mL Microcentrifuge Tubes
Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet on both sides to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic beads
MSR1224B
Minimum Volume 0.2 mL PCR Tube – 10 µL
Maximum Volume 0.2 mL PCR Tube – 0.2 mL
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Minimum Volume 1.5 mL Microcentrifuge Tube – .5 mL
Maximum Volume 1.5 mL Microcentrifuge Tube – 1.5 mL
Permagen’s 0.2 mL PCR / 1.5 mL Microcentrifuge tube separation rack is designed for labs with both PCR strip and 1.5 mL separation protocols. On the top, hold up to two of either 8 or 12 strips, or up to 24 individual tubes. Flip over and hold up to twelve 1.5 mL Microcentrifuge Tubes
