Primer and probe mix (150 reactions) Reverse Transcription, target specific primers (RNA genome viruses only) Copy number standard curve (sufficient for multiple standard curves) Internal extraction control – Read through VIC channel* Endogenous control (150 tests) RNAse/DNAse free water *alternative fluorophores available on request
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Other Products
96-Well 0.2 mL PCR Strip Rack SBS/ SLAS Footprint
Product Info
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Product Info
The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with our MSP750 MSP750RMSPU650R or MSPU650 magnet plates and is available as a discounted kit Picture shown is SKU: MSPU650RKT for reference
The Permagen 96-well 0.2 mL PCR Strip Rack was designed for use in manual or automation applications where strip tubes are required. It can also be used with
p53, also known as tumor protein 53 or TP53, is a tumor suppressor and transcription factor that functions in a number of anti-cancer activities including DNA repair, cell-cycle arrest, and apoptosis in response to DNA damage or other stressors. Mutations in p53 are linked to a number of malignant tumors, including those of the breast, ovarian, bladder, colon, lung, and melanoma. Anti-p53 staining has been used to detect intratubular germ cell neoplasia, and also to distinguish between uterine serous carcinoma and endometrioid carcinoma.
HiPure PCR Pure Micro Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution
Applications
PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
PCR products, enzymatic reaction solution
Sample amount
10-200μl
Recovery
≥80%
Elution volume
≥7μl
Time per run
≤10 minutes
Liquid carrying volume per column
800µl
Binding yield of column
10µg
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
High recovery efficiency – ≥80% DNA recovery
Fast – isolation can be completed in 10 minutes by column gel method
Kit Contents
Contents
D212002
D212003
Purification Times
100 Preps
250 Preps
Buffer DP
60 ml
120 ml
Buffer DW2
20 ml
50 ml
Elution Buffer
15 ml
30 ml
HiPure DNA Micro Columns
100
250
2 ml Collection Tubes
100
250
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Document
HiPure PCR Pure Micro Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
