Overview

• Detection kits for HPV (High and Low Risk)
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s HPV (High and Low Risk) TaqMan PCR Lyophilized Kit is designed for the detection of HPV High and Low Risk specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s HPV (High and Low Risk) TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of HPV High and Low Risk specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM31550L (100 preps)	Cat. TM31510L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)	4 x 350 μL	–
Mycoplasma Primer & Probe Mix (Lyo)	280 μL	280 μL
Mycoplasma Positive Control (Lyo)	150 μL	150 μL
Nuclease-Free Water (Negative Control)	3 x 1.25 mL	1 x 1.25 mL
Product Insert	1	1

Introduction

This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.

Details

Kit Contents

Contents	D635901D	D635902D	D635903D
Purification Times	48	96	480
MagPure Particles N	1.1 ml	2.2 ml	11 ml
Buffer ATL	40 ml	80 ml	2 x 200 ml
Buffer BGL	40 ml	80 ml	2 x 200 ml
Buffer BST1	60 ml	120 ml	550 ml
Buffer GW2*	20 ml	25 ml	2 x 50 ml
Proteinase K	48 mg	110 mg	2 x 220 mg
Protease Dissolve Buffer	5 ml	10 ml	30 ml
DTT	235 mg	235 mg	3 x 235 mg
Elution Buffer	10 ml	10 ml	30 ml

Prefilled Kit Contents

32/48 channel machine

Product	Contents and volume	D6359D-TL-06
Buffer BGL		80 ml
Buffer ATL		80 ml
Proteinase K		110 mg
Protease Dissolve Buffer		10 ml
DTT Powder		2 x 235 mg
Elution Buffer		5 ml
AS-Tip		12 pcs
2.0ml V bottom plate	Row 1/7：empty	6 plates
	Row 2/8：500μl Buffer BST1
	Row 3/9：500μl Buffer BST1
	Row 4/10：20μl MagPure Particles N 500μl Buffer GW2 Row 5/11：500μl Buffer GW2 (ethanol ~350ul)
	Row 6/12：60μl Elution Buffer

96 channel machine

Product	Contents and volume	D6359D-F-96	D6359D-F-48
Buffer BGL		80 ml	40 ml
Buffer ATL		80 ml	40 ml
Proteinase K		110 mg	48 mg
Protease Dissolve Buffer		10 ml	5 ml
DTT Powder		235 mg	235 mg
Elution Buffer		5 ml	5 ml
96-Tip (AS)		1	1
Sample Plate 1	500μl Buffer BST1	1	1
Sample Plate 2	500μl Buffer BST1	1	1
Wash Plate 1	20μl MagPure Particles N500μl Buffer GW2	1	1
Wash Plate 2	500μl Buffer GW2	1	1
Elute Plate	60μl Elution Buffer	1	1

Storage and Stability

Proteinase K and DTT Powder should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these condition.

This product is specially designed for DNA extraction from forensic samples such as bone, hair, fingernail, seminal strain, dry blood spot, ect. Especially designed for forensic detection. The obtained DNA can be directly used for PCR, STR detection and other down stream applications.

Overview

• Detection kit for Toxoplasma gondii
• Available in TaqMan format for analysis

Toxoplasma gondii is a single-celled organism which causes Toxoplasmosis, the most common parasitic infection worldwide affecting animals as well as humans. Cats are the only animal in which sexual reproduction of the organism occurs, and for this reason cats are the only domestic animal which has the potential to shed T. gondii eggs. When disease does occur, it may develop following primary infection due to an inadequate immune response to stop the invasive tachyzoites or as a result of a reactivated infection due to a compromised immune system. The most common symptoms of the disease are anorexia, weight loss, lethargy, dyspnea, ocular symptoms and pyrexia. As toxoplasmosis can be transmitted to humans it represents a serious health risk for people living in close contact with infected animals. Infection is especially dangerous for people with suppressed immune systems and cancer patients as well as pregnant women.

Toxoplasma gondii TaqMan PCR Kit,
100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Toxoplasma gondii TaqMan PCR Probe/Primer Set and Controls,
100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions

All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	TaqMan Probe Cat. TM44750 (100 preps)	TaqMan Probe Cat. TM44710 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 µL	–
Toxoplasma gondii Primer Probe Mix	280 µL	280 µL
Toxoplasma gondii Positive Control	150 µL	150 µL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1