CO00014 Midstream Cassette 140x15mm

Introduction

Intended Use

For the cultivation of E. coli in fermentation and molecular genetic studies.

Principle and Interpretation

Peptone provides nitrogen . Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Glucose provides carbon.Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent.

Formulation

Ingredients	/liter
Peptone	10.0g
Yeast extract	5.0g
Sodium chloride	5.0g
Glucose	1.0g
Agar	15.0g
pH7.0±0.2 at 25°C

Preparation

Suspend 36g in 1 litre of distilled water. Bring to a boil to dissolve completely. Sterilize by autoclaving at 121°C for 15minutes.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 24 hours

Quality control strains	Growth
Escherichia coli  ATCC25922	good

Storage and Shelf Life

2-30℃，Keep container tightly closed, avoid direct sunlight.

Use before expiry date on the label.

 Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Propargyl-PEG1-NHS ester is an amine reactive reagent that can be used for derivatizing peptides, antibodies, amine coated surfaces etc. The alkyne group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG1-NHS ester is an amine reactive reagent that can be used for derivatizing peptides, antibodies, amine coated surfaces etc. The alkyne group reacts with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.