CO00014 Midstream Cassette 140x15mm

Description 

The ExcelTaq™ 5X/2X PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of templates and primers. This not only saves valuable time in the laboratory, but also reduces pipetting and reagent handling errors. The PCR Master Mix is supplied as a 5X/2X concentrated ready-to-use mixture of recombinant Taq DNA Polymerase, reaction buffer, MgCl₂ (TP1120 contains MgSO4), dNTP, and enzyme stabilizer enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent conveniently. This product is supplied with 6X DNA Loading Dye (Blue) containing two tracking dyes (Xylene cyanol FF and Bromophenol blue) for post PCR analysis through the use of agarose gel electrophoresis.

Features

• 5’→3’ DNA polymerase activity
• No detectable 3’→5′ exonuclease (proofreading) activity
• Generates PCR products with 3′-dA overhangs
• High yield PCR 
• High reproducibility
• Reduced pipetting errors

Applications 

• Routine PCR
• Colony PCR
• High throughput PCR
• Amplification of DNA fragments up to 8 kb
• Generation of PCR products for TA cloning
• DNA labeling

Storage

4°C for 6 months
-20°C for 24 months

The ExcelTaq™ 5X/2X PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of templates and primers. This not only saves valuable time in the laboratory, but also reduces pipetting and reagent handling errors. The PCR Master Mix is supplied as a 5X/2X concentrated ready-to-use mixture of recombinant Taq DNA Polymerase, reaction buffer, MgCl2 (TP1120 contains MgSO4), dNTP, and enzyme stabilizer enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent conveniently. This product is supplied with 6X DNA Loading Dye (Blue) containing two tracking dyes (Xylene cyanol FF and Bromophenol blue) for post PCR analysis through the use of agarose gel electrophoresis.

NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free

Conforms to SBS and ANSI requirements

Centrifugal strength up to 3000g

Square well

50 count

Labware definition is available for immediate use in Opentron’s Labware Library

NEST 2 mL 96-Well Deep Well Plate, v-bottom, polypropylene, sterile, DNase, RNase, pyrogen and endotoxin free

Conforms to SBS and ANSI requirements

Centrifugal strength up to 3000g

Square well

50 count

Labware definition is available for immediate use in Opentron’s Labware Library

Name of Product

Echinococcus multilocularis (Em2-Em18) – IgG ELISA

Catalog Number

AF 9300

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Echinococcus multilocularis in human Serum.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample bind to Echinococcus multilocularis Em2-Em18 antigens sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.

Brief Instructions

Storage

2-8°C

Components

ELISA wells, dilution buffer, washing solution, control sera, conjugate, substrate solution, stopping solution

12 x 8 strips (96 tests)

This ELISA kit is intended for the quantitative detection of IgG antibodies against Echinococcus multilocularis in human Serum.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.