Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Detail
Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Other Products
HCM078 Sabouraud Dextrose Broth
Product Info
Document
Product Info
Introduction
Usages:
For cultivations of yeasts , moulds and acidophilic bacteria.
Principle:
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; lactose are fermentable sugars; sodium chloride maintains osmotic equilibrium; the 3rd bile salts and crystal violet inhibiting gram-positive bacteria, especially against Gram positive bacteria and fecal streptococci; neutral red as pH indicator.
Formulation(per liter):
Peptone 5g
Tryptone 5g
Glucose 20g
Final pH 5.7 ± 0.1
How to use:
1.Suspend 30g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Quality control:
Item
The name and number of strain
Growth
Colony Color
1
Aspergillus niger ATCC16404
Good
Spore surface growth
2
Candida albicans ATCC10231
Good
Cloudy broth
3
Escherichia coli ATCC25922
Inhibition
—
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
It’s widely used in the field of animal and plant molecular biology, which is the ideal instrument for seperating the chemical substances in the cell and purifing the microorganisms, viruses, bacteira and subcelluar etc.
TG18 High Speed/Low Speed Centrifuge Features:
1. Max. Speed 21000rpm, max. volume 4x800ml, can be used for 5ml/10ml/15ml/30ml/50ml/100ml centrifuge tubes.
2. Brushless DC motor in great torque, no powder pollution, free maintenance, quick in speed up and down.
3. Can store 10 programs, automatically calculate and synchronously display the RCF data. Digital display indicates the speed, time, temperature and RCF. The parameters can be changed in operation, no need to stop the centrifuge which is quite convenient.
4. The centrifuge body is made of high-quality steel, and with high-quality steel centrifuge chamber, safe and reliable.
5. Automatically electric lid lock, over speed/over temperature protection and imbalance protection.
6. There are many kinds of rotors for your choice, both in high speed and low speed.
TG18 High Speed/Low Speed Centrifuge Technical Parameters:
Max. Speed
21000rpm
Max. RCF
30910xg
Max. Capacity
4x800ml
Timer
0-99min
Speed Accuracy
±20rpm
Noise(DB)
≤65DBA
Voltage(V/HZ)
AC220V/110V,50HZ 10A
Dimension(LxWxHmm)
685x500x385mm
Net Weight(Kg)
70KG
Certificates
CE, ISO
Warranty
1 Year
TG18 High Speed/Low Speed Centrifuge Matched Rotors:
Order No.
Rotor Type
Max Speed(r/min)
Volume(ml)
Max. RCF(xg)
G18-1
Swing Rotor
4000
4x800ml
3450
G18-2
Microplate Rotor
4000
4x4x96well
2940
G18-3
Microplate Rotor
4000
2x4x96well
3210
G18-4
Microplate Rotor
4000
2x3x48well
2300
G18-5
Swing Rotor
4000
4x30x5ml vacuum tube
2840
G18-6
4x30x7ml vacuum tube
3140
G18-7
4x18x10ml vacuum tube
3140
G18-8
Swing Rotor
4000
Fat bottle
3830
G18-9
Fixed Rotor
16000
4x8PCR
15760
G18-10
Fixed Rotor
15000
6x8PCR
21420
G18-11
Fixed Rotor
16000
8x8PCR
17480
G18-12
Fixed Rotor
15000
12x8PCR
22930
G18-13
Fixed Rotor
15000
40×0.5ml
22920
G18-14
Fixed Rotor
21000
12×1.5/2ml
30910
G18-15
Fixed Rotor
16000
24×1.5/2ml
23440
G18-16
Fixed Rotor
14000
30×1.5/2ml
20800
G18-17
Fixed Rotor
13000
48×1.5/2ml
17930
G18-18
Fixed Rotor
16000
16x5ml
22020
G18-19
Fixed Rotor
16000
6x10ml
21500
G18-20
Fixed Rotor
15000
12x10ml
22680
G18-21
Fixed Rotor
13000
16x10ml
19490
G18-22
Fixed Rotor
13000
8x15ml
17790
G18-23
Fixed Rotor
11000
12x15ml
14330
G18-24
Fixed Rotor
5000
24x15ml
3500
G18-25
Fixed Rotor
5000
30x15ml
3830
G18-26
Fixed Rotor
14000
6x30ml
19060
G18-27
Fixed Rotor
13000
6x50ml(conical)
18840
G18-28
Fixed Rotor
13000
6x50ml(round)
18730
G18-29
Fixed Rotor
5000
12x50ml
3860
G18-30
Fixed Rotor
4000
24x50ml
2970
G18-31
Fixed Rotor
13000
4x85ml
18940
G18-32
Fixed Rotor
12000
4x100ml
14850
G18-33
Fixed Rotor
10000
6x100ml
11380
G18-34
Fixed Rotor
4000
12x100ml
2970
G18-35
Fixed Rotor
12000
24 capillaries
15800
G18-36
Swing Rotor
15000
4x5ml
19920
G18-37
Vertical Rotor
16000
16x5ml
16540
G18-38
Vertical Rotor
14000
8x30ml
19750
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