Customized & Bulk

Overview

• Fast and easy processing using rapid and convenient spin-columns
• Isolate high quality and high yield DNA
• DNA is free of inhibitors and ready for downstream use including SNP (single nucleotide polymorphism) and short-tandem repeat (STR) genotyping 

This kit provides a rapid method for the isolation and purification of DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. Norgen’s FFPE DNA Purification Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids.  The purified DNA is of high yield and integrity and is free of inhibitors, ready for use in a number of downstream applications including qPCR, mutation screening, microarray analysis, sequencing, single nucleotide polymorphism (SNP) and short-tandem repeat (STR) genotyping.  The protocol can be completed in as little as 1 hour.

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity (gDNA)	10 μg
Maximum Loading Volume Per Spin Column	650 μL
Size of DNA Purified	All sizes > 80 bp
Maximum Amount of Starting Material	5 sections < 20 µm thick paraffin slices 25 mg of unsectioned block

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. This kit is stable for 1 year after the date of shipment.

Component	Cat. 47400 (50 preps)
Digestion Buffer A	25 mL
Buffer RL	30 mL
Wash Solution A	38 mL
Elution Buffer B	15 mL
Proteinase K	12 mg
RNase A	1 tube
gDNA Purification Micro Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

DBCO-PEG2-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose.

DBCO-PEG2-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. The hydrophilic PEG spacer increases the water solubility of the compound. Reagent grade, for research purpose.

*Optical system: equipped with infinity plan achromatic stress-free metallographic objective lens,built-in polarizing ober-vation device

*Observation head device:The lens barrel can be rotated 360 degrees,and the interpupillary distance adjustment range is 50-75MM

*Eyepiece configuration:random eyepiece WF10X /22mm;

*Objective:Infinity  plan  achromatic  strain-free   metallographic  objective;5X,10X,20X,50X

*Loading  platform:Stage:Tripod  XY  composite   mechanical  moving  stage; size 140X120MM, stroke 80X50MM, minimum reading value 0.1mm

Specification configuration	XTL-20A	Trinocular observation head,transmission single light source 5W LED ighting
	XTL-20B	Trinocular observation head,transparent reflection double light source up and down 5W LED lighting
Eyepiece	Wide-angle eyepiece WF10Xfield of view 22mm,optional WF16X,WF25X
Converter	Quadruple Revolving converter
Objective	Infinite field achromatic stress-free objective,5X,10X,20X,50X
Condenser	N.A.1.25 Swinging Abbe condenser with variable light bar
Stage	Double Layer Mechanical Stage,Size1140X120mn,Moving Range :80x50mm
Focusing	Coaxial Coase &Fine adjustment,fine division 0.02mm
Light source	LED 5V lighting,DC1272A power adapter,adjustable brightness
Attachments	Dust bag,Type C power cord

XTL-20 series metallographic microscopes are equipped with infinity plan achromatic stress-free metallographic objective lens and Wide-angle eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, etc. It can be widely used in biology, medicine, industry, agriculture and other fields, and is an ideal instrument for medical, teaching, scientific research and other units
Model:
XTL-20

Brand:
Yingtai