DBCO-C3-PEG4-amine

A custom designed PACE® Genotyping Assay, designed to your target sequence. Compatible with all PACE genotyping master mixes.

About

PACE (PCR Allelic Competitive Extension) genotyping chemistry is a homogeneous, PCR-based allele-specific technology for the analysis of DNA sequence variants, most commonly SNPs (Single Nucleotide Polymorphisms) and Indels (insertion / deletions).

PACE genotyping chemistry is comprised of two parts:

1. PACE Genotyping Assay: two allele-specific forward primers and one common, reverse primer. The allele-specific forward primers each have different 3′ terminal bases reflecting the target variant, and a unique 5’ tail sequence which is incorporated as part of the fluorescent signal mechanism.
2. PACE Genotyping Master Mix: containing all remaining components required for PCR and the generation of fluorescent signals. PACE Genotyping Master Mix contains a novel, universal, fluorescent reporting cassette to produce machine-readable fluorescent signals (FAM and HEX) corresponding to the assay genotypes.

When combined with sample DNA, these components create a PACE Genotyping Reaction, as illustrated in the figure below.

We have extensive knowledge and experience in assay design, especially when it comes to allele-specific PCR. PACE Genotyping Assays are available to purchase either Validated and Unvalidated. Validated assays require customer DNA to validate and optimise, for guaranteed performance. Unvalidated assays are designed in silico and supplied untested.

REQUIRED COMPONENTS

• qPCR machine or Thermocycler + Fluorescent plate reader
• PCR plate or equivalent and appropriate optically clear seal
• Template DNA
• PCR-grade water
• PACE Genotyping Master Mix or PACE 2.0 Genotyping Master Mix

STEPS TO YOUR PACE GENOTYPING ASSAY DESIGN

1. Place your order on this page. Our support team will contact you by email.
2. Fill out an Assay Design Template form with all the information we need to process your custom PACE Genotyping Assay order. We will email you a copy of the template when we first contact you, or your can download a copy here.
3. Email your completed Assay Design Template to support@3crbio.com
4. Using the information you provide us, we will create your PACE Genotyping Assay designs, order the oligos, and send you design sequences.
5. Once we receive the oligos, we assemble the assay(s) and then ship an aliquot to you (unvalidated) or test on your DNA samples before shipping the aliquot to you (validated).

qPCR machine or Thermocycler + Fluorescent plate reader

PCR plate or equivalent and appropriate optically clear seal

Template DNA

PCR-grade water

PACE Genotyping Master Mix or PACE 2.0 Genotyping Master Mix

Description

This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels in the field. 

This is a revolutionary product designed to make Lead testing in water safe, easy, and affordable.  This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels. With reliable results in only 10 minutes, this test kit clearly gives results confirming Lead in water and conforming to the EPA guideline of 15 ppb (µg/L). The kit can test water quality from various sources including faucets, wells (ground water), and home purification systems.

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Standards and Regulations for Lead [ACCLPP 2012]

• EPA: Drinking Water: 15ppb
• FDA: Juice: 50ppb
• EPA: Residential Soil: 400 ppm (play areas), 1200 ppm (non-play areas)
• CPSC: Paint: 90ppm
• FDA: Bottled drinking water: 5ppb

High Blood lead levels (i.e., greater than 700ppb) can cause serious health effects, including seizure, coma, and death.  Blood levels as low as 100ppb have been associated with adverse effects on cognitive development, growth, and behavior among children aged 1-5 years.

Sample method:

1. Take a first-draw sample

Immediately after opening a faucet or valve, collect a 250 mL sample. This sample should be from each tap used for consumption.

2. Take a flush sample

If first-draw sample results show elevated lead levels of 5 ppb or higher, collect a flush sample. To do this, ensure water has not been used for between 8 to 18 hours, then collect the sample at 30 seconds.

3. Take sequential samples

If you want to test a lead service line, collect 8 to 10 sequential samples, depending on how far the line is from the tap.

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• CDC – Centers for Disease Control and Prevention
  CPSC – Consumer Products Safety Commission
  EPA – Environmental Protection Agency
• FDA Takes Steps to Limit Lead in Juice to Further Reduce Exposure to Toxic Elements in Foods

Screening of Lead in water samples 5-10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 15 Minutes