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DBCO-C3-PEG4-amine

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DBCO-C3-PEG4-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Detail

DBCO-C3-PEG4-amine is a PEG linker which contains DBCO and amine moieties. The DBCO group is commonly used in copper-free Click Chemistry reactions. The amine group is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Other Products

Urine Exosome Purification and RNA Isolation Kits

Urine Exosome Purification and RNA Isolation Midi Kit and Components

Overview

  • Purification and enrichment of intact urinary exosomes for functional studies
  • Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
  • Versatile urine input volumes
  • No phenol extractions, Proteinase K treatment, nor carrier RNA required
  • No time-consuming ultracentrifugation, filtration nor special syringes are required
  • No precipitation reagents nor overnight incubation required
  • Compatible with urine from most species
  • Pure exosomes are purified and are free from any other RNA-binding proteins
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin
  • The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services

Norgen’s Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Urine Exosome Purification and RNA Isolation Mini Kit

For sample volumes ranging from 250 µL to 1 mL.

Urine Exosome Purification and RNA Isolation Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Exosome Purification and RNA Isolation Maxi Kit

For sample volumes ranging from 11 mL to 30 mL.

Details

Supporting Data

Figure 1 / 5

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Figure 1. Urine Exosome Purification and RNA Isolation Mini Kit
Figure 1. Urine Exosome Purification and RNA Isolation Mini Kit
Figure 2. Urine Exosome Purification and RNA Isolation Mini Kit
Figure 3. Urine Exosome Purification and RNA Isolation Mini Kit
Figure 4. Urine Exosome Purification and RNA Isolation Midi Kit
Figure 5. Urine Exosome Purification and RNA Isolation Maxi Kit

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Click for expanded view

Kit Specifications
Minimum Urine Input2 mL
Maximum Urine Input10 mL
Size of Exosomes Purified40 nm – 150 nm
Size of RNA PurifiedAll sizes, including miRNA
and small RNA (< 200 nt)
Elution Volume50-100 μL
Time to Complete 10 Purifications35 – 40 minutes
Average Yields*Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
Urine samples stored at -70°C, -20°C or at 4°C will develop some precipitation due to the aggregation of some of the highly abundant proteins in urine. Eliminating these precipitates using centrifugation or filtration may cause the loss of exosomes. Furthermore, these precipitates may affect the quality of the purified nucleic acid. We recommend the use of Norgen’s Urine Preservative when collecting urine samples, which is designed for the preservation of nucleic acids and proteins in fresh urine samples at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins. Norgen’s Urine Preservative is available as a liquid format in Norgen’s Urine Preservative Single Dose Ampules, as well as in a dried format in Norgen’s Urine Collection and Preservation Tubes.

ComponentCat. 58400 (50 preps)Cat. 58700 (25 preps)Cat. 58800 (15 preps)
Slurry E12.5 mL12.5 mL12.5 mL
ExoC Buffer8 mL30 mL50 mL
ExoR Buffer12 mL12 mL12 mL
Lysis Buffer A20 mL20 mL20 mL
Lysis Additive B2 mL2 mL2 mL
Wash Solution A18 mL18 mL18 mL
Elution Solution A6 mL6 mL6 mL
Mini Filter Spin Columns502515
Mini Spin Columns502515
Collection Tubes502515
Elution Tubes (1.7 mL)1005030
Product Insert111

Magnetic Bead Separation Racks

Description

  • Powerful magnets quickly secure beads
  • Simply turn tube for efficient bead mixing

Different sizes:

  • • 1.5 mL 4 and 16 Tube Rack
  • • 15 mL Tube Rack
  • • 50 mL Tube Rack
  • • 96 well Plate Rack

OT-2 Filter Tips, 20µL

Opentrons OT-2 Filter Tips, 20µL. Optimized for volumes 1µL to 20µL. Clear Tips, Polypropylene tip, Polyethylene Filter Mesh, RNase-/DNase-free, Nonpyrogenic, Protease free. Pre-sterilized with electron beam irradiation. Not Autoclavable.

Compatible with: Opentrons 8-Channel GEN2 P20 Electronic Pipettes, Opentrons Single-Channel GEN2 P20 Electronic Pipettes, Opentrons 8-Channel GEN1 P10 Electronic Pipettes