endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

endo-BCN-PEG2-PFP ester is a click chemistry active ester. The PFP esters have similar applications as the NHS esters, but are more stable in aqueous solution. They can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The BCN group can react with azide-tagged biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

HiPure PCR Pure Mini Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 70bp-30kbp with yields exceeding 85%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Compared with the gel purification kit, this product uses guanidine hydrochloride mediated adsorption, which have better A260/A230 ratio.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments >100bp from various pcr products, enzymatic reaction solutions, labeling reaction solutions, crude DNA, etc.
Applications	PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	PCR products, enzymatic reaction solution
Sample amount	10-200μl
Recovery	≥85%
Elution volume	≥15μl
Time per run	≤10 minutes
Liquid carrying volume per column	800µl
Binding yield of column	35µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – ≥85% DNA recovery
• Fast – isolation can be completed in 10 minutes by column gel method

Kit Contents

Contents	D212102	D212103
Purification Times	100 Preps	250 Preps
Buffer DP	60 ml	120 ml
Buffer DW2	20 ml	50 ml
Elution Buffer	15 ml	30 ml
HiPure DNA Mini Columns II	100	250
2 ml Collection Tubes	100	250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.

Experiment Data

HiPure PCR Pure Mini Kit uses proprietary chemistry and HiPure technology to recover DNA Fragments between 70bp-30kbp with yields exceeding 85%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Compared with the gel purification kit, this product uses guanidine hydrochloride mediated adsorption, which have better A260/A230 ratio.

TG18 Tabletop 21000rpm High Speed Low Speed Non-refrigerated Large Capacity Multi-purpose lab centrifuge 800ml /500ml bottles & 5/7/10/15/50ml tubes. And holding Angle Rotors like 24×1.5/2ml, 6x50ml, 12x15ml…in high speed separation smooth.

TG18 Universal Refrigerated Centrifuge Application:

It’s widely used in the field of animal and plant molecular biology, which is the ideal instrument for seperating the chemical substances in the cell and purifing the microorganisms, viruses, bacteira and subcelluar etc.

TG18 High Speed/Low Speed Centrifuge Features:

1. Max. Speed 21000rpm, max. volume 4x800ml, can be used for 5ml/10ml/15ml/30ml/50ml/100ml centrifuge tubes.

2. Brushless DC motor in great torque, no powder pollution, free maintenance, quick in speed up and down.

3. Can store 10 programs, automatically calculate and synchronously display the RCF data. Digital display indicates the speed, time, temperature and RCF. The parameters can be changed in operation, no need to stop the centrifuge which is quite convenient.

4. The centrifuge body is made of high-quality steel, and with high-quality steel centrifuge chamber, safe and reliable.

5.  Automatically electric lid lock, over speed/over temperature protection and imbalance protection.

6. There are many kinds of rotors for your choice, both in high speed and low speed.

TG18 High Speed/Low Speed Centrifuge Technical Parameters:

Max. Speed	21000rpm
Max. RCF	30910xg
Max. Capacity	4x800ml
Timer	0-99min
Speed Accuracy	±20rpm
Noise(DB)	≤65DBA
Voltage(V/HZ)	AC220V/110V,50HZ 10A
Dimension(LxWxHmm)	685x500x385mm
Net Weight(Kg)	70KG
Certificates	CE, ISO
Warranty	1 Year

TG18 High Speed/Low Speed Centrifuge Matched Rotors:

Order No.	Rotor Type	Max Speed(r/min)	Volume(ml)	Max. RCF(xg)
G18-1	Swing Rotor	4000	4x800ml	3450
G18-2	Microplate Rotor	4000	4x4x96well	2940
G18-3	Microplate Rotor	4000	2x4x96well	3210
G18-4	Microplate Rotor	4000	2x3x48well	2300
G18-5	Swing Rotor	4000	4x30x5ml vacuum tube	2840
G18-6			4x30x7ml vacuum tube	3140
G18-7			4x18x10ml vacuum tube	3140
G18-8	Swing Rotor	4000	Fat bottle	3830
G18-9	Fixed Rotor	16000	4x8PCR	15760
G18-10	Fixed Rotor	15000	6x8PCR	21420
G18-11	Fixed Rotor	16000	8x8PCR	17480
G18-12	Fixed Rotor	15000	12x8PCR	22930
G18-13	Fixed Rotor	15000	40×0.5ml	22920
G18-14	Fixed Rotor	21000	12×1.5/2ml	30910
G18-15	Fixed Rotor	16000	24×1.5/2ml	23440
G18-16	Fixed Rotor	14000	30×1.5/2ml	20800
G18-17	Fixed Rotor	13000	48×1.5/2ml	17930
G18-18	Fixed Rotor	16000	16x5ml	22020
G18-19	Fixed Rotor	16000	6x10ml	21500
G18-20	Fixed Rotor	15000	12x10ml	22680
G18-21	Fixed Rotor	13000	16x10ml	19490
G18-22	Fixed Rotor	13000	8x15ml	17790
G18-23	Fixed Rotor	11000	12x15ml	14330
G18-24	Fixed Rotor	5000	24x15ml	3500
G18-25	Fixed Rotor	5000	30x15ml	3830
G18-26	Fixed Rotor	14000	6x30ml	19060
G18-27	Fixed Rotor	13000	6x50ml(conical)	18840
G18-28	Fixed Rotor	13000	6x50ml(round)	18730
G18-29	Fixed Rotor	5000	12x50ml	3860
G18-30	Fixed Rotor	4000	24x50ml	2970
G18-31	Fixed Rotor	13000	4x85ml	18940
G18-32	Fixed Rotor	12000	4x100ml	14850
G18-33	Fixed Rotor	10000	6x100ml	11380
G18-34	Fixed Rotor	4000	12x100ml	2970
G18-35	Fixed Rotor	12000	24 capillaries	15800
G18-36	Swing Rotor	15000	4x5ml	19920
G18-37	Vertical Rotor	16000	16x5ml	16540
G18-38	Vertical Rotor	14000	8x30ml	19750

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