DBCO-C5-PEG12-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. DBCO group is a highly reactive cycloalkyne which is commonly used for copper-free Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. Reagent grade, for research use only.
DBCO-C5-PEG12-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. DBCO group is a highly reactive cycloalkyne which is commonly used for copper-free Click Chemistry reactions. The hydrophilic PEG chain allows for increased water solubility of compounds in aqueous media. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. HATU) to form a stable amide bond. Reagent grade, for research use only.
The 23S ribosomal RNA (rRNA) is a crucial component of the bacterial/archean ribosome. The 23S rRNA is a 2,904-nucleotide long component of the large subunit (50S) of the ribosome. Compared to 16S rRNA genes, 23S rRNA genes have greater sequence variations due to unique insertions, deletions, and length. Attogene’s 23s PCR kit is designed for identification of specific strains of bacterial/archean using the ribosomal RNA gene region for use in Phylogenetic studies of bacterial diversity from environmental DNA samples such as water. For example, a sample of algae is obtained and washed to extract a clean algal genomic DNA (gDNA) sample. A reaction mixture is assembled from primers, master mix, and gDNA samples as required. The qPCR machine of choice is set up and loaded as needed and the mixture undergoes PCR amplification. The Primer mix provided exploits the Taq polymerase to amplify the gene region of interest.
This kit is sufficient for 150 reactions:
For characterizing cyanobacteria in environmental samples
Use in combination with Attogene Algae DNA isolation kit
Universal 23s PCR primers
Perfect for Environmental DNA (eDNA) Characterization
| Clone | IHC001 |
| Source | Mouse Monoclonal |
| Positive Control | Tonsil, Lymph Node |
| Dilution Range | 1:200 |
Programmed Death 1 (PD-1) is a member of the CD28/CTLA-4 family of T-cell regulators, expressed as a co-receptor on the surface of activated T-cells, B-cells, and macrophages. New studies have suggested that the PD-1/PD-L1 signaling pathway may be linked to anti-tumor immunity, as PD-L1 has been shown to induce apoptosis of activated T cells or inhibit activity of cytotoxic T cells. In comparison to CD10 and Bcl-6, PD-1 is expressed by fewer B cells and has therefore been considered a more specific and useful diagnostic marker for angioimmunoblastic T-cell lymphoma. Therapies targeted toward the PD-1 receptor have shown remarkable clinical responses in patients with various types of cancer, including non–small-cell lung cancer, melanoma, and renal-cell cancer.
RNA/DNA/Protein Purification Plus Kit
This kit provides a rapid method for the high throughput isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, or yeast. The kit employs two columns: 1) for gDNA purification and 2) for RNA purification utilizing Norgen’s resin columns (superior for the binding of all RNA sizes including miRNA). The proteins are also purified on the second column after RNA elution. The proteins are eluted in buffer and are ready for downstream application without any further clean up required. The proteins can be quantified directly, used in western blots, ELISA or mass spectrometry. This kit provides a rapid spin-column method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants.
RNA/DNA/Protein Purification Plus Micro Kit
This kit provides a rapid spin-column method for the isolation and purification of total RNA, DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, microdissected samples including LCM, stem cells, sorted cells, and CTC. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes. The RNA and DNA can be eluted in as little as 20 µL while the protein can be eluted in as little as 50 µL. This kit provides the same performance as if the samples were isolated from dedicated kits.
RNA/DNA/Protein Purification 96-Well Plus Kit
The kit employs two plates: 1) for DNA purification and 2) for RNA purification utilizing Norgen’s resin (superior for the binding of all RNA sizes including miRNA). Please see the protocol schematic below.
Figure 1 / 4
Click for expanded view
| Kit Specifications | |
| Maximum Column Binding Capacity | 35 μg for RNA 10 μg for DNA 100 μg for protein |
| Maximum Column Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Size of DNA Purified | ≥ 30 kb |
| Time to Complete 10 Purifications | 30 minutes |
| Maximum Amount of Starting Material:Animal CellsAnimal TissuesBloodPlant / Fungi Tissues | 5 x 105 cells5 mg (for selected tissues)50 µL25 mg |
| Average Yield* HeLa Cells (5 x 105 cells) HeLa Cells (5 x 105 cells) HeLa Cells (5 x 105 cells) | RNA: 7.5 μg DNA: 1-2 μg Protein: 70 μg |
Storage Conditions and Product Stability
The Protein Loading Dye should be stored at -20°C after the addition of DL-Dithiothreitol (DTT). All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 47700 (50 preps) | Cat. 51600 (50 preps) | Cat. 51700 (96 preps) |
|---|---|---|---|
| Buffer SKP | 40 mL | 40 mL | – |
| Lysis Buffer Q | – | – | 40 mL |
| Wash Solution A | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL | 2 x 38 mL 1 x 18 mL |
| Elution Solution A | 6 mL | 6 mL | 20 mL |
| Elution Buffer F | 15 mL | 15 mL | 2 x 15 mL |
| Wash Solution C | 30 mL | 30 mL | 60 mL |
| Binding Buffer A | 8 mL | 8 mL | 8 mL |
| Elution Buffer C | 8 mL | 8 mL | 30 mL |
| Protein Neutralizer | 4 mL | 4 mL | 4 mL |
| Protein Loading Dye | 2 mL | 2 mL | 3 x 2 mL |
| gDNA Purification Columns | 50 | – | – |
| gDNA Purification Micro Columns | – | 50 | – |
| gDNA Purification 96-Well Plate | – | – | 1 |
| RNA/Protein Purification Columns | 50 | – | – |
| RNA/Protein Purification Micro Columns | – | 50 | – |
| RNA/Protein Purification 96-Well Plate | – | – | 1 |
| Collection Tubes | 150 | 150 | – |
| Collection Plate | – | – | 5 |
| Elution Tubes (1.7 mL) | 150 | 150 | – |
| Elution Plate | – | – | 3 |
| Lysis Preparation Plate | – | – | 2 |
| Adhesive Tape | – | – | 4 |
| Product Insert | 1 | 1 | 1 |
