DBCO-NHCO-PEG13-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-NHCO-PEG13-acid is an analog of DBCO-Acid with a hydrophilic PEG spacer arm, which improves water solubility. This reagent is a non-activated building block with enhanced solubility in aqueous media used to derivatize amine-containing molecule through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
P1156 HiPure Plasmid EF Maxi Kit
Product Info
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Product Info
Introduction
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture
Applications
Cell transfection, animal injection, etc.
Purification method
Maxi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Conventional plasmid vector
Sample amount
High copy plasmid vector: 100-150ml culture mediumLow copy plasmid vector: 150-200ml culture medium
Yield
200-1500μg
Elution volume
≥0.7ml
Time per run
≤60 minutes
Liquid carrying volume per column
20ml
Binding yield of column
1mg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method,then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – silica gel column purification is much faster than ion exchange
High yield – up to 1.5mg plasmid can be binded in one column
Ultra low endotoxin – content of endotoxin is less than 0.1 EU/μg, which can be directly used for cell transfection and animal injection, etc.
Kit Contents
Contents
P115602
P115603
Purification Times
10 Preps
50 Preps
RNase A
30 mg
150 mg
Buffer P1
100 ml
500 ml
Buffer P2
100 ml
500 ml
Buffer LN3
50 ml
250 ml
Buffer PW1
33 ml
180 ml
Buffer PW2
20 ml
100 ml
Elution Buffer
15 ml
120 ml
Buffer CL
33 ml
180 ml
HiPure DNA Maxi Columns C
10
50
Lysate Clear Midi Syringe
10
50
50 ml Collection Tubes C
20
100
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A,Buffer P1 is stable for 6 months when stored at 2–8°C.
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
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Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
50 & 150 reactions