DBCO-NHCO-PEG4-t-butyl ester

Permagen’s 0.2 mL PCR / 1.5 mL Microcentrifuge tube separation rack is designed for labs with both PCR strip and 1.5 mL separation protocols. On the top, hold up to two of either 8 or 12 strips, or up to 24 individual tubes. Flip over and hold up to twelve 1.5 mL Microcentrifuge Tubes

Features include solid aluminum alloy design with hard coat finish for years of trouble-free use, rubber feet on both sides to help prevent slipping on work bench, less tippy than common plastic products, and fast separations using any magnetic bead

SKU #

MSR1224B

Features

• Aluminum alloy design for years of trouble-free use
• Fast magnetic bead separations
• Held to tighter tolerances for more consistent results
• Pulls beads to side of wells for easy tip tracking down opposite side
• Rubber feet to help prevent slippage
• Stable design
• Angled tube holes which allow the tubes to come close to magnets

Compatibility

• Most 1.5 mL Microcentrifuge tubes
• Some 2.0 mL Microcentrifuge tubes
• 8-strip PCR tubes
• 12-strip PCR tubes
• Individual PCR tubes 
• Any Magnetic Beads

Volumes

Maximum (PCR Tube)– .2 mL 

Minimum (PCR Tube)  – 10 µL 

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Maximum (1.5 mL Microcentrifuge) – 1.5 mL 

Minimum (1.5 mL Microcentrifuge Tube) – .5 mL

Permagen’s 0.2 mL PCR / 1.5 mL Microcentrifuge tube separation rack is designed for labs with both PCR strip and 1.5 mL separation protocols. On the top, hold up to two of either 8 or 12 strips, or up to 24 individual tubes. Flip over and hold up to twelve 1.5 mL Microcentrifuge Tubes

Overview

• Isolate DNA from a wide range of food materials. (e.g boiled, fluid, processed or raw food products)
• No hazardous chemicals required (e.g. phenol or chloroform)
• Effective lysis with Proteinase K and optional lysozyme treatment
• Fast (less than 15 minutes hands-on time) and convenient processing using a rapid spin-column format
• Wide compatibility with a variety of food products for GMO-DNA isolation
• Universal protocol for food related pathogen DNA isolation (Gram positive and Gram negative)

This kit provides a rapid spin column method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. The kit is designed for identification of GMO-DNA or animal components in food and feed and can be used for a wide range of starting materials including raw or processed food, meat, liquids, sauces and dairy products including milk, cheese and yogurt.

This kit also provides a convenient method for the detection of food-related pathogens and will isolate such DNA (enriched or as is) including Gram-positive bacteria, Gram-negative bacteria, yeast and fungi which may contaminate food sources.  A number of pathogens have been tested including E. coli O157:H7, Staphylococcus, Listeria monocytogenes, Salmonella enterica & Campylobacter jejuni.  The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection, sequencing and genotyping.  

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Maximum Amount of Starting Material: Solid food material Liquid sample (e.g. milk or concentrated juice)	200 mg 1 mL to 1.5 mL
Time to Complete 10 Purifications	45 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.

Samples Tested by PCR

Food Materials	Samples that Have Been Tested by PCR
Plant related	Cereal, Jam, Chocolate, Spices, Sauce
Animal related	Raw and processed meat products (e.g. ham, beef jerky, taco seasoned ground beef, pork and sausage)
Dairy product	Milk, Yogurt, Cheese
Pathogens (enriched from food samples)	E. coli O157:H7 from food sample Staphylococcus from milk Listeria monocytogenes from milk Salmonella enterica from raw meat Campylobacter jejuni from milk

Component	Cat. 54500 (50 preps)
Lysis Buffer L	60 mL
Binding Buffer I	7 mL
Buffer SK	30 mL
Wash Solution A	18 mL
Elution Buffer B	8 mL
Proteinase K	1 vial
Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1