DBCO-NHCO-PEG4-t-butyl ester

Introduction

Intended Use

For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials

Principle and Interpretation

Enzymatic digest of casein and enzymatic digest of animal tissue provides nitrogen source, vitamins and growth factors; sodium chloride maintains balanced osmoticpressure; sorbitol is a fermentable sugar; No. 3 bile salt and crystal violet inhibit Gram-positive bacteria, potassiumtellurite inhibits non-o157 Escherichia coli, and cefixime inhibits Proteus; neutral red is a pH indicator; and agar is the coagulant of the culture medium.

Formulation

Preparation

Weigh 51.5g of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, dispense into Erlenmeyer flasks, and sterilize at 121℃ for 15min. Melt the sterilized culture medium and cool it to about 45℃, add 1 tube of supporting reagent (SR0240) (0.25mg potassium tellurite and 0.005mg Cefixime) to every 100mL of basal culture medium, mix and pour into the plate.

Quality Control

Cultural characteristics observed after incubation at 35-37°C for 18-24hours

Sorage and Shelf Life

Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

Precautions

1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort

2. Keep container tightly closed after using to prevent clumping.

Waste Disposal

Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.

Intended Use For the isolation and differentiation of sorbitol-negative Escherichia coli serotype O157 from food and animal feed and other materials Principle and Interpretation Enzymatic ……

Name of Product

HIT Control

Catalog Number

MQCOHIT 1

Short Info

The HIT Control (positive and negative) can be used for internal quality Control of the corresponding Milenia QuickLine HIT-Test (MQHIT 1/Z)

This product is only available in Germany!

Method/Platform

HIT-Lateral flow assay

Range/Assay Sensivity

positive and negative control

Test Principle

IgG antibodies are resposible for the heparin induced thrombocytopenia.

Immobilized anti-human IgG on the membrane of the test unit binds patients/control IgG-antibodies which are previously captured by the PF4/polyanion-complex which is detected by intensely colored gold nanoparticles.

The presence of PF4/polyanion-complex becomes visible at a colored test line. The surplus of gold particles continues to migrate through the membrane and is captured at the control line by specific antibodies.

sufficient for 50 tests

The HIT Control (positive and negative) can be used for internal quality Control of the corresponding Milenia QuickLine HIT-Test (MQHIT 1/Z)

N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.

N-(Boc-PEG4)-N-bis(PEG4-propargyl) is a trifunctional chemical containing two terminal alkynes and a Boc-protected primary amine. The terminal alkynes are used in copper click chemistry with azides to form stable triazole linkages with the target molecule while the carboxylic acids are reactive towards alcohols and primary amines to form esters and amides respectively.