DBCO-NHS

Overview

• Detection kits for Candida albicans
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

Candida albicans is an opportunistic yeast and is the most common fungal pathogen found in the human body. C. albicans can be detected in the gastrointestinal tract, mouth, and vagina of approximately half of healthy adults. It is typically a commensal organism and makes up part of the natural human microflora; however it can overgrow and become pathogenic as a result of various conditions. For example, individuals who have taken recent courses of antibiotics, have a weakened immune system or have diabetes have an increased risk of developing a Candida albicans infection. When an overgrowth occurs, this can lead to common infections such as urinary tract infections, genital yeast infections, oral thrush, and mucocutaneous candidiasis. In the more severe cases, when Candida albicans enters the bloodstream or organs, it can lead to loss of sight, blood and bone infections, endocarditis, meningitis or inflammation of the intraabdominal lining.

Candida albicans TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Candida albicans TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM34050 (100 preps)	Cat. TM34010 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Candida albicans Primer & Probe Mix	280 μL	280 μL
Candida albicans Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Description 

The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers, TaqMan probes and templates. The master mix includes a 5’ to 3’ exonuclease activity to cleave TaqMan probes that hybridize to target sequences, releasing fluorophore during probe displacement. With TaqMan probes, the master mix features high specificity and high sensitivity. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) contains hot-start Taq polymerase in an optimized buffer that allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for the normalization of each qPCR assay. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.

Features

• High Specificity
• High Sensitivity
• With ROX Reference Dye

Storage

Protect from light. 
Aliquot to avoid multiple freeze-thaw cycles. 
-20°C for 12 months

The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers, TaqMan probes and templates. The master mix includes a 5’ to 3’ exonuclease activity to cleave TaqMan probes that hybridize to target sequences, releasing fluorophore during probe displacement. With TaqMan probes, the master mix features high specificity and high sensitivity. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) contains hot-start Taq polymerase in an optimized buffer that allows for sensitive and precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. The master mix includes ROX reference dye for the normalization of each qPCR assay. The ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process.

GL20 Technical Parameter：

Max. Speed	21000rpm
Max. RCF	30910×g
Max. Capacity	4×800ml
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 60
Temperature	-20℃~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	680×540×830mm
Net Weight(Kg)	170KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for GL20

Order No	Rotor	Rotor Max Speed(r/min)	Volume(ml)	RCF(×g)
G20-1	Swing Rotor	4000	4×800ml	3450
G20-2	Microplate rotor	4000	4×4×96 well	2940
G20-3	Microplate rotor	4000	2×4×96 well	3210
G20-4	Microplate rotor	4000	2×3×48 well	2300
G20-5	Swing rotor	4000	4×40×7ml vacuum tube	3270
G20-6		4000	4×40×5ml vacuum tube	2890
G20-7		4000	4×28×10ml vacuum tube	3130
G20-8	Swing Rotor	4000	Fat bottle	3830
G20-9	Fixed Rotor	16000	4×8PCR	15760
G20-10	Fixed Rotor	15000	6×8PCR	21420
G20-11	Fixed Rotor	16000	8×8PCR	17480
G20-12	Fixed Rotor	15000	12×8PCR	22930
G20-13	Fixed Rotor	15000	40×0.5ml	22920
G20-14	Fixed Rotor	21000	12×1.5ml	30910
G20-15	Fixed Rotor	16000	24×1.5ml	23440
G20-16	Fixed Rotor	14000	30×1.5ml	20800
G20-17	Fixed Rotor	13000	48×1.5ml	17930
G20-18	Fixed Rotor	16000	16×5ml	22020
G20-19	Fixed Rotor	16000	6×10ml	21500
G20-20	Fixed Rotor	15000	12×10ml	22680
G20-21	Fixed Rotor	13000	16×10ml	19490
G20-22	Fixed Rotor	13000	8×15ml	17790
G20-23	Fixed Rotor	11000	12×15ml	14330
G20-24	Fixed Rotor	5000	24×15ml	3500
G20-25	Fixed Rotor	5000	30×15ml	3830
G20-26	Fixed Rotor	14000	6×30ml	19060
G20-27	Fixed Rotor	13000	6×50ml(conical)	18840
G20-28	Fixed Rotor	13000	6×50ml(round)	18730
G20-29	Fixed Rotor	5000	12×50ml	3860
G20-30	Fixed Rotor	4000	24×50ml	2970
G20-31	Fixed Rotor	13000	4×85ml	18940
G20-32	Fixed Rotor	12000	4×100ml	14850
G20-33	Fixed Rotor	10000	6×100ml	11380
G20-34	Fixed Rotor	4000	12×100ml	2970
G20-35	Fixed Rotor	12000	24 capillaries	15800
G20-36	Swing Rotor	15000	4×5ml	19920
G20-37	Vertical rotor	16000	16×5ml	16450
G20-38	Vertical rotor	14000	8×30ml	19750

Features

1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control

3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.

4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.

6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.

7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.