DBCO-PEG10-DBCO

Propargyl-PEG10-NHS ester serves as a bifunctional PEG linker with a terminal propargyl that participates in click reactions with azide-bearing moieties and an NHS ester group that reacts readily and efficiently with amine-bearing molecules. The PEG10 units improve the water solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG10-NHS ester serves as a bifunctional PEG linker with a terminal propargyl that participates in click reactions with azide-bearing moieties and an NHS ester group that reacts readily and efficiently with amine-bearing molecules. The PEG10 units improve the water solubility of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Character of Yingtai laboratory scale freeze dryer

Small size, and light weight

Compact construction of tabletop freeze dryer

Suitable for small samples drying

Advantage of Yingtai 3kgs smallest lab scale freeze dryer 

3kg water capture capacity, which can meet the needs of general laboratory freeze-drying capacity

-70°C ultra-low temperature cold trap, which can capture conventional organic solvents

Direct-capture coil cold trap with higher efficiency and better ice-catching effect

The cold trap and condenser coil are all Teflon anti-corrosion treatment, which can freeze-dry organic solvents

Large flat flange type silicone seal, effectively guarantee the vacuum tightness, no need to apply any auxiliary sealing silicone grease forever

One-button operation, the host and the vacuum pump are linked, no manual operation required

The Pirani vacuum sensor with automatic temperature compensation can accurately detect the vacuum degree in the chamber at each stage

Removable sample shelf with special holes for centrifuge tubes, making it easier to place and adjust the height

Various types of chambers and T-frame configurations can fully meet the user’s sample placement

Vacuum pumps and sample placement chambers can be freely combined according to user needs, making the configuration more flexible and convenient

Techinical parameter

Item No	BD1-3	BD1-6	BD2-6
24/h water capture capacity	3kg	6kg	6kg
Cold trap temperature	-70℃	-70℃	-90℃
Cold trap volume	5L	10L	10L
Freeze dried chamber	High permeability organic glass/multi manifold external valve/stainless steel chamber
Sample shelf	φ 200mm stainless steel with 3-5 layers available	●Stainless steel φ 260mm/485mm. 3-10 layers/optional●Electric heating/manual capping/optional
vacuum pump	Standard configuration
Oil mist filter	Extreme vacuum 4 * 10-4mbar,with a pumping capacity of 78L~200L/optional pump of various types
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
anti-corrosive treatment	Cold trap, condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents
External valve	6/optional	1-48 pieces/optional
Eutectic point detection system	Support/Optional
Data output analysis	Support/Optional
Power	0.6kw	1.1kw	0.5kw

The NGS Low Input DNA Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality NGS libraries with low input DNA amount from 1 ng to 400 ng. The kit allows scientist to study samples with limited DNA such as tumor samples, patient samples, and other specially collected samples (FACS sorting etc.). The kit has high library conversion efficiency with as little as 1 ng DNA input. The fast and simple 1.5-hour protocol makes libraries with even coverage and low GC-bias based our unique chemistry for DNA end-polishing and ligation.

The kit needs double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic approach (for example, BioDynami DNA fragmentation enzymes, Cat.# 40061 and Cat.# 40062) and mechanical approaches such as sonication and nebulization etc.

NGS Low Input DNA Library Prep Kit Workflow

Three index types are available for the NGS Low Input DNA Library Prep Kit of illumina platform:

Non-index (Cat.# 30022): Libraries do not have index.

Index (Cat.# 30024): Each of our index primers contains a unique barcode sequence with 6 bases that can be used to identify the low input DNA libraries. Library multiplexing up to 48 samples is possible. Index information can be downloaded here.

Unique dual index (Cat.# 30025): Library multiplexing up to 96 low input DNA libraries is possible with unique dual indexes with our Four-Base Difference Index System. The system allows us to make indexes for the libraries that have at least 4 bases different from each other in the 8-base barcode length. Our unique dual index primers can reduce sequencing errors such as de-multiplexing errors, amplification errors, mis-assignment of reads, index cross-contamination, and also index hopping. The kit  includes 96 pre-mixed unique pairs of index primers. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34024).

Kit advantages:

• • • Fast protocol
      • The hands-on time is only 10 minutes
      • The total protocol time is around 1.5 hours
    • Simple procedure
      • Ready-to-use master mix makes it simple for reaction setup
      • Less reaction components
    • Less magnetic beads required for cleanup steps: Save the cost more than 50%
    • Low input DNA amount: Starts from 1 ng of DNA

Comparison of library conversion efficiency under the same NGS library preparation condition. Input DNA amounts are 1 ng and 10 ng, respectively. DNA was mechanical sheared with Covaris before library prep. BioDynami kit: NGS Low Input DNA Library Prep Kit (Cat. #30022).

Comparison of library yield under the same NGS library prep condition. Input DNA amounts are 1 ng, 10 ng, and 100 ng. DNA was mechanical sheared with Covaris before library prep. BioDynami kit (Cat. #30022). PCR cycle numbers were indicated.

The NGS Low Input DNA Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality NGS libraries with low input DNA amount from 1 ng to 400 ng. The kit allows scientist to study samples with limited DNA such as tumor samples, patient samples, and other specially collected samples (FACS sorting etc.). The kit has high library conversion efficiency with as little as 1 ng DNA input. The fast and simple 1.5-hour protocol makes libraries with even coverage and low GC-bias based our unique chemistry for DNA end-polishing and ligation.