endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
endo-BCN-PEG6-t-butyl ester is a click chemistry linker. The hydrophilic PEG spacer increases solubility in aqueous media. The BCN group can react with azide-tagged molecules. The protected carboxyl group (COOH) prevents self coupling or polymerization under standard acid/amine or acid/hydroxyl coupling conditions. It can be converted to free acid under acidc condition. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Product image shows functional testing of Protein A 40nm colloidal gold on a lateral flow test. Protein A 40nm colloidal gold was incubated in lateral flow running buffer alone or in the presence of a limiting amount of monoclonal antibody that targets the chemical-BSA conjugate. The gold was then applied to the sample port of a cassette containing a lateral flow strip sprayed with a chemical conjugate (Chemical-BSA) on the test line and a goat anti mouse antibody as the control line. The data demonstrates the specificity and robustness of the Protein A 40nm colloidal gold.
Our products are produced in a state-of-the-art manufacturing facility that enable rapid turnaround times while ensuring batch to batch consistency and reliability.
Document
Functionally tested Protein A conjugated gold Perfect for Lateral Flow Development and use in final product 40nm gold particles conjugated to Protein A are offered in 10 OD 1mL size. If a different OD or amount is required please
EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits
Product Info
Document
Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Purification and enrichment of intact cell culture media exosomes for functional studies
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free-from any other RNA-binding proteins
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
40-45 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
EXTRAClean Urine Exosome Purification and RNA Isolation Kits
Product Info
Document
Product Info
Overview
Ensure optimal results for sensitive applications like NGS
Up to a tenfold increase in microRNA mapping during sequencing runs to reduce costs
Purification and enrichment of intact urinary exosomes for functional studies.
Bind and elute all RNA irrespective of size or GC content, without bias.
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
No phenol extractions, Proteinase K treatment, nor carrier RNA required.
No time-consuming ultracentrifugation, filtration nor special syringes are required.
No precipitation reagents nor overnight incubation required.
Compatible with urine from any species.
Pure exosomes are purified and are free-from any other RNA-binding proteins.
Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix.
Norgen’s EXTRAClean Urine Exosome Purification and RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of RNA from different urine sample volumes ranging from 250 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, NGS application, Northern blotting, RNase protection and primer extension, and expression array assays.
All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume
50-100 μL
Time to Complete 10 Purifications
35-40 minutes
Average Yields
Variable depending on specimen
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.