DBCO-PEG12-acid

Overview

• No need to immediately process samples
• Total DNA, including viral DNA, preserved at room temperature over 4 months
• Total RNA, including viral RNA, preserved at room temperature over 2 months
• Inactivate microorganisms including bacteria, fungi, yeast and viruses
• Inactivates SARS-CoV-2 virus – Explore the Application Note
• Ship swab samples at room temperature safely
• Compatible with most DNA and RNA isolation methods
• CE-IVD marked version available

Norgen’s Total Nucleic Acid Preservation Tubes are designed for ambient preservation and transport of total nucleic acids (DNA and RNA) from samples collected using a swab, including nasal, buccal, saliva, fecal, skin, surfaces, etc. The Total Nucleic Acid Preservation Tubes contain Norgen’s Total Nucleic Acid Preservative in a liquid format. The user simply collects the specimen using a sterile nylon flocked swab (not provided), and then transfers the swab into the Total Nucleic Acid Preservative. The preservative prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the Total Nucleic Acid Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Preservative allow DNA samples to be stored at room temperature for over 4 months and RNA samples to be stored at room temperature for over 2 months.
Swab samples collected and preserved using Norgen’s Total Nucleic Acid Preservation Tubes are compatible with most commercially available DNA and RNA isolation methods. Samples stored in the tubes have been used successfully with most of Norgen Biotek’s DNA and RNA isolation kits and reagents, including Norgen’s Total RNA Purification Kit (Cat# 17200) and Norgen’s Saliva DNA Isolation Kit (Cat# RU45400). For purification of total nucleic acids please refer to the supplementary protocol provided with Norgen’s Total RNA Purification Kit (Cat#17200).

Details

Supporting Data

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Kit Specifications
Stability of DNA at Room Temperature	4 months
Stability of RNA at Room Temperature	2 months

Storage Conditions and Product Stability

The Total Nucleic Acid Preservation Tubes can be stored at room temperature for up 1 year without any reduction in kit performance. The collect before date is indicated on the tube label and the kit box label.

Kit Components	Cat. 69200
Total Nucleic Acid Preservation Tubes	50
Product Insert	1

Propargyl-PEG6-NHS ester is an amine reactive linker. The alkyne in this linker can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reaction. The PEG spacer increases solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG6-NHS ester is an amine reactive linker. The alkyne in this linker can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reaction. The PEG spacer increases solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Detection kits for Vibrio cholerae
• Lyophilized format designed for room temperature shipping
• Available in TaqMan format for analysis

Norgen’s Vibrio cholerae TaqMan PCR Lyophilized Kit is designed for the detection of Vibrio cholerae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Norgen’s Vibrio cholerae TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Vibrio cholerae specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.

Details

Supporting Data

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Storage Conditions and Product Stability

• All kit components should be stored at -20°C upon arrival.
• Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
• Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
• All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

Component	Cat. TM38550L (100 preps)	Cat. TM38510L (100 preps)
MDx TaqMan 2X PCR Master Mix (Lyo)	4 x 350 μL	–
Vibrio cholerae Primer & Probe Mix (Lyo)	280 μL	280 μL
Vibrio cholerae Positive Control (Lyo)	150 μL	150 μL
Nuclease-Free Water (Negative Control)	3 x 1.25 mL	1 x 1.25 mL
Product Insert	1	1