DBCO-PEG12-DBCO

Name of Product

Echinococcus multilocularis (Em18) – IgG ELISA

Catalog Number

AF 9310

Short Info

This ELISA kit is intended for the quantitative detection of IgG antibodies against Em-18 antigen of Echinococcus multilocularis in human serum.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Method/Platform

ELISA in microplate format

Range/Assay Sensivity

pNPP, λ=405 nm

Test Principle

Specific antibodies in the sample bind to Echinococcus multilocularis Em18 antigen sensitized on microtiter plates. The presence of parasite specific antibodies is detected with a Protein A – alkaline phosphatase conjugate.

12 x 8 strips (96 tests)

This ELISA kit is intended for the quantitative detection of IgG antibodies against Em-18 antigen of Echinococcus multilocularis in human serum.

This product is manufactured by Bordier Affinity Products in Switzerland and distributed in Germany exclusively by Milenia Biotec.

Overview

• Purification and enrichment of intact plasma/serum exosomes for functional studies
• Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
• Versatile plasma/serum input volume
• No phenol extractions, Proteinase K treatment, nor carrier RNA required
• No time-consuming ultracentrifugation, filtration nor special syringes required
• No precipitation reagents, nor overnight incubation required
• Compatible with plasma/serum from most species
• Pure exosomes are purified and are free from any other RNA-binding proteins
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin
• The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services

Norgen’s Plasma/Serum Exosome and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Plasma/Serum Exosome and RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and RNA Isolation Midi Kit

For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Norgen’s Plasma/Serum Exosome and RNA Isolation Maxi Kit

For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Details

Supporting Data

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Kit Specifications
Minimum Plasma/Serum Input	4 mL
Maximum Plasma/Serum Input	10 mL
Size of Exosomes Purified	40 nm – 150 nm
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35 – 40 minutes
Average Yields*	Variable depending on specimen

*Please check page 4 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Component	Cat. 58300 (50 preps)	Cat. 58500 (25 preps)	Cat. 58600 (15 preps)
Slurry E	12.5 mL	12.5 mL	12.5 mL
ExoC Buffer	8 mL	8 mL	2 x 8 mL
ExoR Buffer	12 mL	12 mL	12 mL
Lysis Buffer A	20 mL	20 mL	20 mL
Lysis Additive B	2 mL	2 mL	2 mL
Wash Solution A	38 mL	18 mL	18 mL
Elution Solution A	6 mL	6 mL	6 mL
Mini Filter Spin Columns	50	25	15
Mini Spin Columns	50	25	15
Collection Tubes	50	25	15
Elution Tubes (1.7 mL)	100	50	30
Product Insert	1	1	1

The NGS Library Quantification Standards with PCR Primers (for illumina platform) were developed for quantification of the NGS library concentration for illumina sequencing platform. It is comprised of Library Standards (six 10-fold dilutions) and a primer mix.

Quantification of the NGS library of the amplifiable molecules is critical for the quality of the sequencing data. Adequate library concentration will maximize sequencing capacity. Poor library concentration results in either low or high cluster density on the flow cell, which can lead to low sequencing capacity.

It is not accurate to measure the concentration of NGS library with standard DNA quantification methods such as spectrophotometer or fluorometer. QPCR is the best way for library quantification with high consistency and reproducibility of library quantitation.

BioDynami Library Quantification Standards with PCR Primers (for illumina platform): Amplification curve of 6 standards.

Our reagent is a highly sensitive, Real time PCR-based quantification that specifically designed for NGS libraries using illumina sequencing platform. The amplification uses illumina adaptor sequences as primers, and only the fully adaptor-ligated libraries will be amplified.  Therefore the reagent provides an accurate estimation of the library concentration based on true sequenceable illumina libraries. In addition, this kit can also be used for confirmation of ligation reaction after completion of library preparation.

Our library quantification standards is compatible with commercial SYBR Green based QPCR reagents. This makes it more flexible for scientists who want to use their real time PCR reagent. Quantification of library concentration is achieved by comparison with a standard curve generated from the Library Standards.

The NGS Library Quantification Standards with PCR Primers (for illumina platform) were developed for quantification of the NGS library concentration for illumina sequencing platform. It is comprised of Library Standards (six 10-fold dilutions) and a primer mix.