DBCO-PEG12-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG12-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from all types of soil samples including common soil samples and difficult soil samples with high humic acid content such as compost and manure. A combination of chemical and physical homogenization effectively lyses all microorganisms in the soil sample, and the kit removes all traces of humic acid using the provided Organic Substance Removal (OSR) Solution and Humic Acid Removal plate (HAR). Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and qPCR applications and more for any metagenomic study, as all humic acid substances and PCR inhibitors are removed during the isolation.
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| Kit Specifications | |
| Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material | 250 mg |
| Time to Complete 96 Purifications | 50 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 26560 (192 preps) |
|---|---|
| Lysis Buffer G | 2 x 100 mL |
| Lysis Additive A | 25 mL |
| Binding Buffer I | 25 mL |
| OSR Solution | 12 mL |
| Wash Solution A | 2 x 38 mL |
| Elution Buffer B | 30 mL |
| Bead Tubes | 200 |
| HAR Plate | 2 |
| 96-Well Filter Plate | 2 |
| Adhesive Tape | 4 |
| 96-Well Collection Plate | 4 |
| 96-Well Elution Plate | 2 |
| Product Insert | 1 |
Usages:
For cultivating and enriching non-fastidious bacteria, and for evaluating the efficacy of disinfection.
Principle:
Peptone and beef extract powder provide nitrogen, vitamins, amino acids and carbon; sodium chloride to maintain osmotic equilibrium.
Formulation（per liter）:
Peptone 10.0g
Beef Extract 3.0g
Sodium Chloride 5.0g
Final pH 7.4 ± 0.2
How to use：
1.Suspend 18g in 1L of distilled water, stirring heated to boiling to completely dissolve, autoclave at 121℃ for 15 minutes.
2.Diluted and treated samples.
Quality control：
| Item | The name and number of strain | Growth | Colony Color |
| 1 | Escherichia coli ATCC25922 | Good | Turbid broth,slight precipitate |
| 2 | Staphylococcus aureus ATCC25922 | Good | Turbid broth,slight precipitate |
Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Specifications: 250g/bottle
250g
The Opentrons 24-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block acts as a 24 position rack to keep 2mL tubes, 1.5mL tubes, and the NEST 0.5mL tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
The Opentrons 24-well Aluminum Block can be placed directly on the OT-2/Opentrons Flex deck or on the Opentrons Temperature Module. This block acts as a 24 position rack to keep 2mL tubes, 1.5mL tubes, and the NEST 0.5mL tubes at constant temperature. The aluminum block holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).
