DBCO-PEG12-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG12-DBCO is a monodisperse click chemistry linker containing two terminal DBCO groups with hydrophilic PEG spacer arm. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
m-PEG2-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
m-PEG2-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments >100bp from agarose gel(<0.3g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA |
| Applications | PCR, NGS, chip analysis, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Agarose Gel, PCR products, enzymatic reaction solution |
| Sample amount | Agarose Gel: <0.3g, PCR products: ≤50µl |
| Recovery | 80% |
| Elution volume | ≥30μl |
| Time per run | ≤30 minutes |
The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
Kit Contents
| Contents | D500101 | D500102 | D500103 |
| Purification Times | 50 Preps | 500 Preps | 5000 Preps |
| Buffer GDP | 30 ml | 250 ml | 3 x 900 ml |
| MagPure Particles | 1.6 ml | 15 ml | 3 x 50 ml |
| Buffer DW1 | 20 ml | 180 ml | 2 x 800 ml |
| Elution Buffer(10mmTris, pH8.5) | 10 ml | 60 ml | 500 ml |
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) provides a fast and reproducible method for isolating genomic DNA from saliva samples collected and preserved using Norgen’s Saliva DNA Collection and Preservation Devices, as well as fresh saliva. Saliva DNA purified using Norgen’s kit is of the highest quality, and is compatible with a number of downstream research applications including PCR, Southern Blot analysis, sequencing and microarray analysis.
Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Figure 1 / 6
Click for expanded view
| Kit Specifications | |
| Maximum Saliva Input | 0.5 mL preserved saliva 0.25 mL fresh saliva |
| Average Yield from 0.25 mL of Saliva* | 2-5 μg |
| Average Purity (OD260/280) | 1.6 – 1.8 |
| Time to Complete 10 Purifications | 30 minutes (Cat. RU55400) 45 minutes (Cat. RU62900) |
* Average DNA yield will vary depending on the donor
Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature (15 – 25°C). These reagents should remain stable for at least 2 years in their unopened containers.
| Component | Cat. RU55400 (50 preps) | Cat. RU62900 (192 preps) |
|---|---|---|
| Lysis Buffer F | 30 mL | 2 x 30 mL |
| Proteinase K in Storage Buffer | 1.2 mL | 4 mL |
| Magnetic Bead Suspension | 2 x 1.1 mL | 8.5 mL |
| Elution Buffer B | 8 mL | 30 mL |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Plate | – | 2 |
| 96-Well Elution Plate | – | 2 |
| Adhesive Tape | – | 2 |
| Product Insert | 1 | 1 |
