DBCO-PEG12-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-PEG12-Maleimide is a PEG linker containing a DBCO moiety and a terminal primary maleimide group. The maleimide group will react with a thiol group to form a covalent bond, enabling the connection of biomolecule with a thiol. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
R5111 HiPure DNA/RNA Kit
Product Info
Document
Product Info
Introduction
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Details
Specifications
Features
Specifications
Main Functions
Co-isolation DNA and RNA(not include miRNA) from a single sample (cells, soft tissue, plant sample)
Applications
RT-PCR, cDNA synthesis, PCR andsecond-generation sequencing, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Soft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues
Sample amount
Soft Tissue: < 30mg, Cell: <1 x 107, Plant: <100mg
Yield
DNA: 1 – 20 μg, RNA: 3 – 100 μg
Principle
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Advantages
High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
Fast – column method can complete the extraction of several samples in 30 minutes
Safe – no phenol chloroform extraction required
Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Kit Contents
Contents
R511102
R511103
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Columns
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RLC
50 ml
200 ml
Buffer DW1
30 ml
150 ml
Buffer RW1
30 ml
150 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Buffer AE
10 ml
50 ml
Storage and Stability
HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
TCO-PEG6-DBCO is a PEG6 linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine groups in target compounds.
Document
TCO-PEG6-DBCO is a PEG6 linker featuring a trans-cyclooctene and a DBCO group. DBCO is a click chemistry handle which easily reacts with azides, while the TCO function readily reacts with tetrazine groups in target compounds.