DBCO-PEG2-DBCO

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Primer and probe mix (150 reactions)
Reverse Transcription, target specific primers (RNA genome viruses only)
Copy number standard curve (sufficient for multiple standard curves)
Internal extraction control – Read through VIC channel*
Endogenous control (150 tests)
RNAse/DNAse free water
*alternative fluorophores available on request

Overview

• Eight discrete bands, ranging from 100 to 1,000 bases
• Higher intensity band at 500 bases for easy reference
• No need for staining or destaining as loading dye contains ethidium bromide
• Convenient lyophilized format provides better product stability

The Norgen 100 b RNA Ladder is a set of RNA transcripts derived from recombinant DNA templates. This ladder is suitable for precise sizing of small RNA molecules using native or denaturing agarose gels, and can be easily visualized under UV.

Contents

• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer

Details

Supporting Data

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Specifications:

• Eight discrete bands, ranging from 100 to 1,000 bases
• Higher intensity band at 500 bases for easy reference

Contents:

• 2 vials of lyophilized RNA ladder (25 loads each)
• 1x loading buffer: 33.3% formamide, 11.7% formaldehyde, 0.012% bromophenol blue, 0.05mM EDTA, 0.012% ethidium bromide, and 0.7x MOPS solution
• 2x loading buffer

Instructions:

To reconstitute the lyophilized RNA ladder, add 250 µL of 1x loading buffer to each 25 loads vial and vortex gently.
Heat at 80°C for 10 minutes. Incubate on ice for 1 min. Load 10 µL on a 1.5-2% gel. For optimal results, use Norgen 2x loading buffer with each RNA sample. There is no need for staining and destaining denaturing gels since Norgen’s loading buffer contains ethidium bromide.

Storage:

Store at -20°C.

Control  System:   

 5.4”LCD   Touch   Screen   Display

>The display can simultaneously display:all main parameters such as cold trap temperature,vacuum degree,sample temperature, and shelf temperature.

>Schematic diagram of equipment operating components,through graphic display to view the working status of each component of the equipment.

>Ithas  the  functions  of  cold  trap  precooling  and  vacuum  pump  preheating,and  the  time  can  be  set  by  the  userin  the  system. >Display  whether  the  current  running  status  is  normal  or  not  by  numerical  color.

>Built-in    saturated    vapor     pressure    &temperature    corresponding     relationship    table,dearly     displaying    the    corresponding     relationship.

o One-key operation,the operation is extremely simple,no special professional learning and training is required.

o Thermal electromagnetic over-current and short-circuit protection,making the equipment run more safely and stably. 

o Active reminder of equipment maintenance time and vacuum pump replacement lubricating oil time.

o Ambient    temperature    monitoring,the    device    automatically     reminds    the    alarm    if    the    ambient    temperature     is    too    high.

o Optional eutectic point detection device and endpointjudgment detection system make freeze-drying more scientific.

Drying chamber	· The separate drying chamber reduces the probability of damage caused byimproper handling of the drying chamber due to height and weight.The upper cover is anodized aluminum with anti-corrosion treatment.●Highly transparent plexiglass drying chamber,good light and heat radiation conduction.Reserve 6/12 external   valve ports.· 316L stainless steel all black Teflon coating drying chamber,suitable for drying organic solvents and  various corrosive samples,reserved for 6/12 external ports.· 316L stainless steel vertical multi-port multi-manifold external rack(The standard configuration is aplexiglass drying chamber,and one ofthe diying chambers can be optional)
Cold trap andcondensing coil	· The cold trap cavity and condensation coil are made of 316L stainless steel.Large opening and channel design,the gas can be quickly captured through the condensing coil.· The cavity and condensing coil are equipped with Teflon anti corrosion coating as standard,which can trap organic solvents and various corrosive solvents.· The condensing coil is placed in the cold trap to increase the condensate catchment area,which can effectively prevent external condensation.
Sample shelves	· 316Lstainless steel stackable sample shelves,capable oflyophilizing machine solvents and various corrosive  solvents.Each shelf can be placed and taken freely without manual work.The distance and height ofeach shelfcan be adjusted freely.-All black coating,good heat absorption performance.-Special hole for freeze-drying of centrifuge tubes,which is conducive to the drying of samples in centrifugetube containers.· All-aluminum Teflon surface anti-corrosion treatment electric heating shelf.
Refrigerationsystem	· Danffos compressorCopper plate heat exchanger,the largest area to increase heat dissipation· Unique refrigeration technology,the temperature of the cold trap can be lowered from ambient temperatureto below-80C°within 2 minutes.· Class A temperature sensor with high temperature accuracy and low error.· Multiple detection and alarm system,the refrigeration system pressure is too high or refrigeration failure cantimely alarm and take corresponding measures.

Techinical parameter

Item No	ST7003	ST7006		ST9006
Ice condenser capacity	3kg	6kg		6kg
Ice condenser temperature	-70℃	-70℃		-90℃
Cold trap volume	5L	10L		10L
Freeze dried chamber	Bell shape (standard configuration)/Multi manifold type/T-shaped frame type
Sample shelf	φ200mm.Single layer area 0.03m2 with 3-5 layers available	●260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optiona
Vacuum pump	Extreme vacuum 5*10*mbar with a pumping capacity of 8m³/h optional pump of various types
Oil mist filte	Standard configuration
Vacuum pump connection pipe	Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
Anti-corrosive treatment	Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents.
External valve	6pieces/optional	1-48 pieces/optional
Eutectic point detection system	Support/Optional
Data output analysis	Support/Optional
Powe	0.6kw	1.1kw		1.5kw
Size	420*545*455mm		510*570*500mm

Freezer Dryer, 3kgs-6kgs water capturer
Model:
ST7003,ST7006,ST9006

Brand:
Yingtai