DBCO-PEG2-Maleimide

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V1-V2 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V2 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V1-V2 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V2 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

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Minimum amount of starting material:	2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:	4 hours

Storage Conditions and Product Stability
Norgen’s 16S V1-V2 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Step	Component	Cat. 70100 (24 preps)	Cat. 70110, 70120, 70130, 70140 (96 preps)
Amplicon PCR (PCR 1)	MGX Master Mix	330 µL	1,320 µL
	16S V1-V2 Primer Mix	70 µL	280 µL
Index PCR (PCR 2)	Indexing Master Mix	660 µL	2 x 1,320 µL
	N7xx Index Primer	50 µL	50 µL
	S5xx Index Primer	70 µL	70 µL
PCR Clean-Up	Resuspension Buffer	2 x 1,250 µL	2 x 5,000 µL
	Nuclease-free water	1,250 µL	1 x 6,000 µL

The Opentrons® 3-piece Aluminum Block Set is used with our Temperature Deck. The set comes with a 24-well, 96-well and flat bottom plate format block. These are used to keep your 2mL tubes, 1.5mL tubes, 96-well PCR plates, PCR strips and flat bottom plates at a constant temperature. These blocks holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).

The Opentrons® 3-piece Aluminum Block Set is used with our Temperature Deck. The set comes with a 24-well, 96-well and flat bottom plate format block. These are used to keep your 2mL tubes, 1.5mL tubes, 96-well PCR plates, PCR strips and flat bottom plates at a constant temperature. These blocks holds temperatures between 4°C and 95°C when used with the Opentrons Temperature Module (GEN2).

Designed for those labs wishing to separate magnetic beads into rings.

The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies. 

SBS SLAS Footprint (127.75mm x 85.50mm) to fit into any automated liquid handling robot 

Features include solid aluminum alloy construction and hard coat anodized finish for years of trouble-free use, and compatible with any magnetic beads

SKU #

RLE500R

Features

• SBS/ SLAS footprint
• Integrated cushion base for precise pipetting
• Solid Aluminum alloy design
• Precision manufactured for more consistent results
• Fast magnetic bead separations
• Integrated bracket to help keep microplate in place and flat
• Made in USA 
• For Research use only

Compatibility

• Full- skirt PCR plates
• 1/2- skirt PCR plates 
• Un-skirted PCR plates
• Any magnetic beads
• Any common liquid handlers i.e. SPTLabtech Firefly®, Tecan®, Opentrons®, Hamilton®, Agilent®, Beckman®, etc.

Volumes

Maximum  – 150 µL 

Minimum  – 5 µL 

Designed for those labs wishing to separate magnetic beads into rings.

The Permagen ring magnet low elution plate with integrated cushion base plate was designed for use in automation applications where volumes as low as 5 µL are required. Most PCR plates are bent, leading to inconsistent lab results. Unlike most products, we have added an angled frame around the top of our plate, this helps with two things, straightening out the PCR plate, and leading it to the proper location on the magnets during automation while the cushion assist compensates for labware inconsistencies.