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DBCO-PEG4-3-formylbenzamid

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Detail

Molecular structure of the compound BP-41825

Other Products

TD5B Large Capacity Low Speed Centrifuge

TD5B Large Capacity Low Speed Centrifuge

Features:

1. Brushless motor with simpler construction, more reliable performance, longer life and quietly running.
2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.
4. Microprocessor control, TD5B with digital display which indicate the speed, time, RCF in operation, speed raising and reducing quick, operate simply.
5.10 kinds of accelerating and decelerating speed for your choice.

TD5B Technical Parameter

Max. Speed5000rpm
Max. RCF4730×g
Max. Capacity4×800ml
Time Range0~99min
RPM/RCF ConvertYes
Noise (dB)≤ 58
TemperatureNormal
Acc/Dec10 Kinds
Speed Accuracy±20r/min
Temperature Accuracy/
Voltage(V/Hz)AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)685×500×385mm
Net Weight(Kg)67KG
CertificatesCE,ISO & Calibration report are available

Matched Rotors for TD5B

Order NoRotorMax Speed(r/min)Volume(ml)Max RCF(×g)
5B-1Swing Rotor40004×800ml3450
5B-2Swing Rotor40004×750ml3530
5B-3Swing Rotor40004×500ml3380
    5B-4Swing Rotor40004×4×96(well)2940
5B-5Microplate rotor40002×4×96(well)3210
5B-62×3×48(well)2300
5B-7Swing rotor50004×1×50ml4730
4×1×100ml4730
40004×2×100ml3020
40004×2×50ml3200
40004×4×15ml3200
40004×6×15ml3200
5B-8Swing rotor40004×8×15ml3200
40004×12×5ml vacuum tube2480
40004×12×7ml vacuum tube2760
40004×12×10ml vacuum tube2880
40004×8×10/7/5ml vacuum tube2790
40004×6×10/7/5ml vacuum tube2790
40004×4×10/7/5ml vacuum tube2790
5B-9Swing rotor40004×24×5ml vacuum tube2480
40004×31×5ml Khan tubes2480
5B-10Swing rotor40004×24×7ml vacuum tube3140
40004×31×7ml Khan tubes3140
40004×30×7ml vacuum tube3140
40004×30×5ml vacuum tube3140
40004×40×7ml vacuum tube3270
40004×18×10ml vacuum tube3140
5B-11Swing rotor4000Dairy bottle3830
5B-12Fixed rotor50006×15ml2540
5B-13500012×15ml3080
5B-14500024×15ml3500
   5B-15500030×15ml3830
5B-1650004×50ml2520
5B-1750006×50ml2850
5B-18500012×50ml3860
5B-19400024×50ml2970
5B-2050004×100ml2630
5B-2150006×100ml3130
5B-22400012×100ml2970

Adapter for 800ml and 500ml bucket

 VolumeCup5ml7ml10ml15ml50ml
800ml(Round Cup)23pcs/cup2319145
500ml(Round Cup)18pcs/cup181493
500ml(Square Cup)20pcs/cup2016125

RNA Clean-Up and Concentration Kits

Overview

  • Clean-up & concentrate total RNA (including miRNA) in minutes
  • Clean-up & concentrate RNA from TRIzol®, TRI Reagent®, etc
  • Clean-up RNA from contaminants including enzymes, primers, nucleotides
  • Rapid spin-column protocol, elute in 20 µL
  • 96-well format for high throughput processing & Micro-Elute spin column formats for 8 µL are available
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
  • Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

These kits are designed to clean-up and concentrate RNA (including miRNA) from TRIzol® and TRI Reagent®, enzymatic reactions, in vitro transcription, labelling reactions, etc. These are robust kits for all clean-up and concentration purposes for up to 35 µg of RNA in solution. The purified RNA is of the highest purity and integrity, and can be used in a number of downstream applications. These kits purify all sizes of RNA, from large mRNA and ribosomal RNA down to miRNA, siRNA and lncRNA.

RNA Clean-Up and Concentration Kit (Spin Column)

Norgen’s RNA Clean-Up and Concentration Kit provides a rapid method for the purification, cleanup and concentration of up to 35 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 20 µL. Complete 10 purifications in 20 minutes.

RNA Clean-Up and Concentration 96-Well Kit (High Throughput)

Norgen’s RNA Clean-Up and Concentration 96-Well Kit provides a rapid method for the purification, cleanup and concentration of up to 50 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment and labeling. The kit elutes RNA in 75 µL. Complete 10 purifications in 30 minutes.

RNA Clean-Up and Concentration Micro-Elute Kit (Micro-Elute)

Norgen’s RNA Clean-Up and Concentration Micro-Elution Kit provides a rapid method for the purification, cleanup and concentration of up to 10 μg of RNA isolated using different methods including phenol/guanidine-based protocols, and from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. This kit is made for eluting RNA in smaller volumes of 8 µL for all types of downstream applications, for the highest concentration of RNA sample. Complete 10 purifications in 20 minutes.

Protocol

Details

Supporting Data

Figure 1 / 10

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Figure 1. Effective Clean-Up to Produce High Quality Total RNA with Complete Size Diversity
Figure 1. Effective Clean-Up to Produce High Quality Total RNA with Complete Size Diversity
Figure 2. Effective Clean-Up to Produce High Quality Total RNA Compatible to Bioanalyzer Analysis
Figure 3. Effective Concentration and Detection of Low Amounts of RNA Input
Figure 4. Effective Clean-Up to Produce High Quality RNA Transcripts
Figure 5. Effective Clean-Up to Produce High Quality RNA Transcripts Compatible to Bioanalyzer Analysis
Figure 6. Excellent Quality of Concentrated RNA
Figure 7. High Recovery of Concentrated RNA
Figure 8. Concentration of Total RNA
Figure 9. Concentration of miRNA
Figure 10. Concentration of RNA prior to Next Generation Sequencing (NGS) applications

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Kit Specifications (Spin Column)
Maximum Column Binding Capacity35 μg
Size of RNA PurifiedAll sizes, including small RNA
(<200 nt)
Maximum Amount of Starting Material35 μg of RNA
Minimum Elution Volume20 μL
Time to Complete 10 Purifications20 minutes
Average Recovery≥ 90%



Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

ComponentCat. 23600 (50 preps)Cat. 43200 (100 preps)Cat. 25100 (192 preps)Cat. 61000 (50 preps)
Buffer RL40 mL2 x 40 mL2 x 40 mL40 mL
Wash Solution A38 mL2 x 38 mL2 x 38 mL38 mL
Elution Solution A6 mL2 x 6 mL2 x 20 mL6 mL
Column Activation Solution30 mL
Micro Spin Columns50100
Micro-Elute RNA Spin Columns50
96-Well Plate2
Adhesive Tape4
Collection Tubes5010050
96-Well Collection Plate2
Elution Tubes (1.7 mL)5010050
96-Well Elution Plate2
Product Insert1111

All Pathogenic Salmonella Species

Description

Salmonella spp. are members of the family Enterobacteriaceae. They are Gram-negative, facultatively anaerobic, flagellated, rod-shaped organisms. They are approximately 0.7 to 1.5 µm in diameter and 2 to 5 µm in length and responsible for a large number of cases of foodborne illness throughout the world. Salmonella have circular DNA genomes with a mean length of approximately 4530 kb, although this can vary by up 1000 kb. Salmonella classification is extremely complex, however, the genus is divided into two species: S. enterica and S.bongori. S. enterica is then itself divided into 6 biochemically distinct subspecies and the Salmonella genus is further classified into serovars (serotypes) based on the lipopolysaccharide (O), flagella protein (H), and sometimes the capsular (VI) antigens. There are more than 2500 known serovars and within a serovar there may be strains that differ in virulence.

Salmonella are mainly transmitted by the faecal-oral route. They are carried asymptomatically in the intestines or gall bladder of many animals, being continuously or intermittently shed in the faeces. Humans can become infected if they do not wash their hands after contact with infected animals or animal faeces. In such instances the bacteria adhere to and enter the cells of the intestinal epithelium. The toxins produced by the bacteria can damage and kill the cells that line the intestines, which results in intestinal fluid loss. The bacteria can survive for weeks in a dry environment and far longer in water thus they are frequently present in polluted waters. Salmonella can also be carried latently in the mesenteric lymph nodes or tonsils; these bacteria are not shed, but can become reactivated after stress or immunosuppression. In addition, fomites and vectors can spread Salmonella and vertical transmission occurs in birds, with contamination of the vitalize membrane, albumen and possibly the yolk of eggs. Salmonella spp. can also be transmitted in utero in mammals.

There are two different disease conditions that are distinct to salmonellosis; gastroenteritis and enteric typhoid fever. The gastroenteritis is a nonsystemic infection of the intestinal tract and regional lymph nodes that gives rise to headache, muscle aches, diarrhoea, vomiting, abdominal cramping, chills, fever, nausea and dehydration. In contrast, the enteric typhoid fever is a systemic disease in which the microorganism replicates within the cells of the reticuloendothelial system. The symptoms usually appear 6 to 72 hours after ingesting contaminated food although individuals can be infected with the bacteria without having symptoms. Those with and without symptoms shed the bacteria in their stool and it is important that personal hygiene be maintained at all times.