Synovial fluid is secreted into the joint cavity from the inner membrane of synovial joints. Synovial fluid is a plasma ultrafiltrate which contains proteins derived from both the blood plasma and produced by cells within the joint tissues. It lubricates the articulating joints as well as supplies oxygen and nutrients while removing carbon dioxide and metabolic wastes from the chondrocytes in the surrounding cartilage. Septic arthritis is usually caused by bacterial, viral or fungal infections to the synovial fluid. Staphylococcus aureus is the most common bacterial infection causing Septic arthritis. Diagnosing Septic arthritis is done through joint fluid (synovial fluid) analysis, blood tests or imaging tests.
Norgen’s Synovial Fluid Bacterial Genomic DNA Isolation Kit provides a fast, reliable and simple procedure for isolating the highest quality and the highest quantity of bacterial genomic DNA from various amounts of synovial fluid ranging from 1 mL up to 10 mL. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all Gram +ve and Gram -ve strains. Moreover, the kit allows the user to elute the purified bacterial genomic DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified bacterial gDNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis and NGS.
Figure 1 / 2
Click for expanded view
Kit Specifications | |
Maximum Input | 2 x 109 bacterial cells |
Column Binding Capacity | 25 μg |
Average Yield | up to 20 µg |
Time to Complete 10 Purifications | 45 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Component | Cat. 67900 (50 preps) |
---|---|
Precipitation Solution B | 15 mL |
Lysis Buffer T | 6.5 mL |
Solution BX | 28 mL |
Wash Solution A | 38 mL |
Elution Buffer B | 8 mL |
Micro Spin Columns | 50 |
Collection Tubes | 50 |
Elution Tubes (1.7 mL) | 50 |
Product Insert | 1 |
With the development of molecular biology, stool, a new non-invasive sample, has been widely used in the research of animal molecular genetics, population ecology, behavioral ecology and some intestinal disease diagnosis. Stool samples includes gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.
The primary problem encountered when using stool sample for molecular biology research is the low content of exfoliated cells in the digestive tract and a certain degree of degradation of genetic material in stool. Another issue in molecular scatology research based on PCR is the presence of a large number of inhibitors in stool that can affect Taq enzyme activity, leading to downstream detection inactivation. These inhibitors include polysaccharides, plant polysaccharides, bile acids, bile salts, bile pigments, digestive juices, mucus, etc. Therefore, selecting appropriate extraction methods to obtain high-quality DNA is the key to successful downstream detection of stool DNA.
At present, the pretreatment methods used in the laboratory, such as phenol/chloroform extraction, cetyltrimethyl bromide (CTAB) lysis, and guanidine isothiocyanate lysis, lack universality in different species, and the success rate of extracting DNA for PCR amplification is also very low. The HiPure Stool DNA Kit provided by Magen Company has opened up a new approach for DNA extraction from stool samples with good universality, high cost-effectiveness, high yield and purification. The reagent kit adopts a unique solution system and inhibitory factor adsorbent, which can efficiently remove various impurities in stool samples. The purified DNA can be directly used for PCR, quantitative PCR and other applications.
This product allows rapid and reliable isolation of high-quality genomic DNA from various stool samples. Up to 100 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
Specifications
Features | Specifications |
Main Functions | Isolation total DNA from 50-100mg stool samples |
Applications | PCR, Southern Blot, enzyme digestion and NGS, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Stool |
Sample amount | 50-100mg |
Yield | 3-15μg |
Elution volume | ≥30μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 750μl |
Binding yield of column | 100μg |
Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
Kit Contents
Contents | D314102 | D314103 |
Purification Times | 50 Preps | 250 Preps |
HiPure DNA Mini Columns II | 50 | 250 |
2ml Collection Tubes | 50 | 250 |
2ml Bead Tubes | 50 | 250 |
Proteinase K | 24 mg | 120 mg |
Protease Dissolve Buffer | 1.8 ml | 10 ml |
Buffer SPL | 40 ml | 200 ml |
Buffer PCI | 40 ml | 200 ml |
Buffer AL | 20 ml | 80 ml |
Buffer GW1 | 22 ml | 88 ml |
Buffer GW2 | 20 ml | 2 x 50 ml |
Buffer AE | 15 ml | 30 ml |
Storage and Stability
Proteinase K and Buffer PCI should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
With the development of molecular biology, stool, a new non-invasive sample, has been widely used in the research of animal molecular genetics, population ecology, behavioral ecology and some intestinal disease diagnosis. Stool samples includes gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.
The primary problem encountered when using stool sample for molecular biology research is the low content of exfoliated cells in the digestive tract and a certain degree of degradation of genetic material in stool. Another issue in molecular scatology research based on PCR is the presence of a large number of inhibitors in stool that can affect Taq enzyme activity, leading to downstream detection inactivation. These inhibitors include polysaccharides, plant polysaccharides, bile acids, bile salts, bile pigments, digestive juices, mucus, etc. Therefore, selecting appropriate extraction methods to obtain high-quality DNA is the key to successful downstream detection of stool DNA.
At present, the pretreatment methods used in the laboratory, such as phenol/chloroform extraction, cetyltrimethyl bromide (CTAB) lysis, and guanidine isothiocyanate lysis, lack universality in different species, and the success rate of extracting DNA for PCR amplification is also very low. The HiPure Stool DNA Kit provided by Magen Company has opened up a new approach for DNA extraction from stool samples with good universality, high cost-effectiveness, high yield and purification. The reagent kit adopts a unique solution system and inhibitory factor adsorbent, which can efficiently remove various impurities in stool samples. The purified DNA can be directly used for PCR, quantitative PCR and other applications.
This product allows rapid and reliable isolation of high-quality genomic DNA from various stool samples. Up to 100 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.
TGW16 Technical Parameter:
Max. Speed | 16000rpm |
Max. RCF | 19040×g |
Max. Capacity | 10×5ml |
Time Range | 0~99min59s |
RPM/RCF Convert | Yes |
Noise (dB) | ≤ 55 |
Acc/Dec | 10 Kinds |
Speed Accuracy | ±20r/min |
Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
Size (W x D x Hmm) | 355×270×205mm |
Net Weight(Kg) | 16KG |
Certificates | CE,ISO & Calibration report are available |
Matched Rotors for TGW16
Order No | Rotor | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
W16-1 | Angle rotor | 16000 | 40×0.2ml | 19040 |
W16-2 | Angle Rotor | 16000 | 24×0.5ml | 18480 |
W16-3 | Angle Rotor | 16000 | 12×1.5/2ml | 17940 |
W16-4 | Angle Rotor | 16000 | 10x5ml | 17880 |
W16-5 | Angle Rotor | 14000 | 20×1.5/2ml | 15580 |
W16-6 | Angle Rotor | 14000 | 4x8PCR | 12070 |
1. Brushless motor, no pollution, free-maintenane.
2. Microprocessor control, LCD display which indicates the speed, time, RCF in operation, 10 kinds of brake setting , operate simply.
3. Electric lid lock, super speed, imbalance protection.
4. The centrifuge body is made of high quality steel, stainless steel chamber, safe and reliable.
5. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
6. 3 tiers protection steel cover and get the ideal centrifuation result.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map