DBCO-PEG4-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-PEG4-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Portable 4000rpm TD4C Centrifuge Machine for Lab, PRP & PRF
Product Info
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Product Info
Portable 4000rpm TD4C Centrifuge Machine for Lab, PRP & PRF
Application:
Widely used in the laboratory, hospital, clinic and so on.
Product Features:
1. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.
2. Rang of speed from 0~4000rpm, smooth in operation, low noise and small vibration.
3. Micro-computer control system, digital display the RCF, time and speed.
4. Electric cover lock, compact design, super speed and imblance protection.
The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from <150 mg simple plant sample without chloroform
Applications
RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method
Mini spin column
Purification technology
Silica technology, DNA filtration technology
Process method
Manual (centrifugation or vacuum)
Sample type
Economic crops
Sample amount
≤150 mg
Elution volume
≥30μl
Time per run
≤25 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanolis added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
Advantages
Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – several samples can be extracted in 25 minutes by column method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be recovered at the level of PG
Broad spectrum – various types of plant samples can be processed by diversity of operating procedures
Kit Contents
Contents
R415102
D415103
Purification Times
50 Preps
250 Preps
HiPure DNA Mini Column II
50
250
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
100
2 x 250
Buffer RLC
50 ml
200 ml
Buffer PRC1
50 ml
200 ml
Buffer RW1
50 ml
200 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form inthe Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.
Purchase Guide
1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.
2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.
3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.
4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.
Select the right purification kit to get impactful results:
The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Heat sealing film for 96- and 384-well PCR microplates.
About
We are thrilled to announce the latest addition to our consumables range at 3CR Bioscience: heat sealing film for 96- and 384-well PCR plates.
This rigorously tested, permanent seal film complements our rigid frame 384-well PCR plates perfectly. Its superior clarity allows for easy sample visualisation and monitoring, ensuring excellent data quality.
Crafted from high-quality materials, the seal offers excellent chemical resistance and plate compatibility. Designed with durability, the film provides a secure seal with precise fit and strong adhesion, safeguarding sample integrity throughout amplification, re-cycling, and reading.
Both seals and our 384-well plates are ideal for automated, high-throughput PCR environments, including robotic handling. The film remains stable during thermal cycling in PCR machines and hydro cyclers, offering performance and convenience across various PCR workflows.
With this new addition, we reaffirm our commitment to delivering reliable, high-performance, and affordable products that empower scientists worldwide.
Contact us today to include a free sample of plate and seal in your next shipment!
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Heat sealing film for 96- and 384-well PCR microplates.