DBCO-PEG4-C3-sulfonic acid

• This kit is sufficient for 150 reactions:
• For characterizing cyanobacteria in environmental samples
• Use in combination with Attogene Algae DNA isolation kit
• Universal 23s PCR primers
• Perfect for Environmental DNA (eDNA) Characterization

Description

The 23S ribosomal RNA (rRNA) is a crucial component of the bacterial/archean ribosome. The 23S rRNA is a 2,904-nucleotide long component of the large subunit (50S) of the ribosome. Compared to 16S rRNA genes, 23S rRNA genes have greater sequence variations due to unique insertions, deletions, and length.  Attogene’s 23s PCR kit is designed for identification of specific strains of bacterial/archean using the ribosomal RNA gene region for use in Phylogenetic studies of bacterial diversity from environmental DNA samples such as water. For example, a sample of algae is obtained and washed to extract a clean algal genomic DNA (gDNA) sample. A reaction mixture is assembled from primers, master mix, and gDNA samples as required. The qPCR machine of choice is set up and loaded as needed and the mixture undergoes PCR amplification. The Primer mix provided exploits the Taq polymerase to amplify the gene region of interest.

This kit is sufficient for 150 reactions:
For characterizing cyanobacteria in environmental samples
Use in combination with Attogene Algae DNA isolation kit
Universal 23s PCR primers
Perfect for Environmental DNA (eDNA) Characterization

Description

Attogene’s 0.5mL Micro Spin Desalting Columns are convenient, simple, and ready to be used out of the box. We provide a product that facilitates equal, if not superior results to leading brands products at a significantly better cost. Superlative recovery of proteins and other macromolecules (>7000 MW) with greater than 95% retention of salts, and other small molecules (<1000 MW), are possible even with very dilute (25 ug/mL) samples. Our columns, which are constructed with a simple break away tab at the bottom, are comprised of polypropylene and contain our own proprietary resin slurry that we’ve developed in house to achieve optimal results. Sample volumes between 30-130uL can be loaded while still achieving expected purification numbers.

Download Spec Sheet

Rapid, simple, and reliable components for purification of protein samples
Cleanup before or after antibody biotinylation
Made by Attogene in the USA

Propargyl-PEG12-methane is a PEG linker that can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The PEG chain enhances the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG12-methane is a PEG linker that can react with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry to form a stable triazole linkage. The PEG chain enhances the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.