Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Detail
Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Reagent technology of protein/enzyme system: freeze-dried powder, freeze-dried microspheres
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This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39ºC~42ºC), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension.It is suitable for laboratory-level DNA amplification and DNA amplification for other detection purposes.
genesig 32 Overview The genesig q32 Real-Time PCR Instrument is one of the fastest qPCR instruments on the market today due to its rapid heating and cooling. Assembled from just a few building blocks, this robust qPCR instrument allows analysis of up to 32 samples in both tube or strip format. The powerful genesig q32 software is easily installed from a USB flash drive onto any PC or Mac. The genesig q32 is used with 550 genesig Real-Time PCR Kits for human, vet, and food pathogens, as well as food speciation testing
The genesig Real-Time PCR Kit product range includes tests for a massive range of applications:
genesig q32 – Cutting edge design and technology Assembled from just a few building blocks the robust genesig q32 system is easy to transport and install. Up to 32 samples can be run in 0.1 ml tubes or 8-strip format. Fast heating and cooling is achieved by utilizing robust Peltier elements, whilst assay performance is supported further by a heated lid design. Excitation is provided by high intensity LEDs filtered to provide light at 500nm that is capable of exciting all fluorophores commonly used in qPCR. A prism is used to generate spectra from fluorescent emissions. These spectra are imaged using a CMOS camera.
genesig q32 software The genesig q32 software is delivered and easily installed from a USB flash drive. When installed in a network, the software can control several q32 instruments connected via LAN. The powerful and easy to use software provides the following features:
• Quick start using templates for all genesig kit applications • Straightforward setup and editing of sample and target information • Automated analysis modules for absolute and relative quantification, melting curve analysis and endpoint genotyping • Analysis of full spectral data • Compatible with Windows, Mac & Linux Systems • Instrument start from a USB flash drive, using preprogrammed settings • Data export functions
genesig Real-Time PCR Kit compatible genesig Real-Time PCR Kits are suitable for use on the genesig q32 instrument. The genesig Kit range includes over 550 kits for human, vet, and food pathogens, as well as food speciation testing. All genesig Kits include a positive control and resuspension buffers. The kits come freeze-dried so that they can be shipped at room temperature for fast delivery to anywhere in the World.
Guaranteed results using 550 genesig kits Fast DNA and RNA analysis in less than 60 minutes Quick and easy set-up and run Automated data analysis result calling Small and robust instrument Exceptional value for money
MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
Details
Specifications
Features
Specifications
Main Functions
Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP)
Applications
NGS, DNA library
Purification technology
Magnetic beads technology
Process method
Manual (centrifugation or vacuum)
Sample type
DNA products, restriction endonuclease systems, or other enzymatic reaction solutions
Sample amount
Appropriate
Recovery
80%
Operation time
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.
Advantages
High recovery – up to 80%
High throughput – using magnetic beads purification technology
Kit Contents
Contents
BP-5
BP-50
BP-500
MagPure A4 XP
5 ml
50 ml
500 ml
Storage and Stability
MagPure A4 XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 4 weeks) at room temperature (15-25°C) does not affect its performance. — Shake the reagent well before use. It should appear homogenous and consistent in color.
DO NOT FREEZE.
Document
MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
