Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Detail
Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Formaldehyde
Assay Format:
Spectrophotometer, Microplate, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.1 to 14 μg of formaldehyde per assay
Limit of Detection:
0.033 mg of formaldehyde per test or 0.016 mg/mL of formaldehyde in a sample treated as per the standard procedure
Limit of Quantification:
0.11 mg of formaldehyde per test or 0.054 mg/mL of formaldehyde in a sample treated as per the standard procedure
Reproducibility (%):
~ 3%
Reaction Time (min):
~ 15 min
Application examples:
Environmental samples, food and beverage samples.
The Formaldehyde Assay Kit provides a simple robust method for the measurement of formaldehyde.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
The kit provides rapid, simple, and reliable components for the labeling proteins with biotin for use in lateral flow procedures. Kit enables the biotinylation of antibody or proteins to be used with the Universal Lateral Flow Kits (AU2034, AU2037 or AUAU2044 in addition to Streptavidin Colloidal (AU2017) gold or Streptavidin CdZnS Quantum Dots (AU2043).
Document
Attogene’s Protein Biotinylation kit provides the user with reagents for labeling proteins
Desalting columns for purifying the labeled molecule
Convenient and easy to interpret instructions on how to carry out the labeling process.
Made by Attogene in the USA
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Mycobacterium tuberculosis is a pathogenic bacterial species belonging to the genus Mycobacterium, and is the causative agent of tuberculosis. Tuberculosis (TB) is a multifaceted disease and challenging public health problem in both industrialized and developing countries. According to the WHO, 8.8 million active cases of TB are diagnosed each year and of these, almost 2 million die. Once thought to be under control or even close to extinction, TB infection levels are rising and the threat is compounded by new, virulent and drug-resistant strains. Although most cases occur in the developing world (22 countries accounting for 80% of all global cases), increasing population mobility combined with facility of transmission means that no country is immune from the resurgence of TB. TB control programs are currently facing a number of constraints. Worldwide, fewer than 25% of all tuberculosis cases are detected. Of utmost concern is the absence of a timely and accurate test for the diagnosis of mycobacterial disease. Early diagnosis is crucial for the prevention of further spread of the disease.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
