DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Portable 4000rpm TD4C Centrifuge Machine for Lab, PRP & PRF
Product Info
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Product Info
Portable 4000rpm TD4C Centrifuge Machine for Lab, PRP & PRF
Application:
Widely used in the laboratory, hospital, clinic and so on.
Product Features:
1. Brushless motor, free maintenance, no powder pollution, quick in speed up and down.
2. Rang of speed from 0~4000rpm, smooth in operation, low noise and small vibration.
3. Micro-computer control system, digital display the RCF, time and speed.
4. Electric cover lock, compact design, super speed and imblance protection.
Actin is part of the cytoskeletal system of all cell types. Smooth Muscle Actin is found in myofibroblasts and myoepithelium, but not in cardiac and skeletal muscles. Labelling of smooth muscle actin in concert with muscle specific actin staining can allow for differentiation between rhabdomyosarcoma and leiomyosarcoma, as Muscle specific actin is found in rhabdomyoblasts, while smooth muscle actin is found in leiomyosarcomas.
The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Details
Principle
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Kit Contents
Contents
R401601
R401602
Purification Times
1 x 96
4 x 96
HiPure RNA Plate
1
4
1.5ml Collection Plate
1
4
0.5ml Collection Plate
1
4
RTL Lysis Buffer
100 ml
400 ml
RNA Binding Buffer*
30 ml
120 ml
Buffer RW1
100 ml
400 ml
Buffer RW2*
50 ml
2 x 100 ml
RNase Free Water
20 ml
80 ml
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
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The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.