DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Features
1.Brushless frequency motor,in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Microprocessor control
3.Digital display in time, speed and RCF.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
6.High quality steel centrifuge body , stainless steel chamber, 3 tiers protection,safe and reliable.
7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction
TG16 Technical Parameter：
| Max. Speed | 16000rpm |
| Max. RCF | 20600×g |
| Max. Capacity | 6×100ml |
| Time Range | 0~99min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 65 |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (L x W x Hmm) | 513×370×320mm |
| Net Weight/Gross weight(Kg) | 48KG/55kg |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotor for TG16
| Order No | Rotor | Max speed (rpm) | Max Volume(ml) | Max RCF (g) |
| G16-1 | Angle rotor | 14000 | 4×8PCR | 12070 |
| G16-2 | Angle rotor | 13000 | 6×8PCR | 16080 |
| G16-3 | Angle rotor | 14000 | 8×8PCR | 13390 |
| G16-4 | Angle rotor | 13000 | 12×8PCR | 17220 |
| G16-5 | Angle Rotor | 15000 | 30×0.5ml | 18510 |
| G16-6 | Angle Rotor | 14000 | 40×0.5ml | 19970 |
| G16-7 | Angle Rotor | 16000 | 12×1.5/2ml | 17940 |
| G16-8 | Angle Rotor | 15000 | 24×1.5/2ml | 20600 |
| G16-9 | Angle Rotor | 13500 | 30×1.5/2ml | 19340 |
| G16-10 | Angle Rotor | 11000 | 48×1.5/2ml | 12840 |
| G16-11 | Angle Rotor | 15000 | 16×5ml | 19350 |
| G16-12 | Angle Rotor | 14000 | 12×7ml | 16370 |
| G16-13 | Angle Rotor | 14000 | 6×10ml | 16460 |
| G16-14 | Angle Rotor | 13000 | 12×10ml | 14510 |
| G16-15 | Angle Rotor | 10000 | 12×15ml | 11840 |
| G16-16 | Angle Rotor | 5000 | 24×15ml | 3080 |
| G16-17 | Angle Rotor | 5000 | 30×15ml | 3830 |
| G16-18 | Angle Rotor | 12000 | 8×20ml | 14510 |
| G16-19 | Angle Rotor | 12000 | 6×30ml | 14000 |
| G16-20 | Angle Rotor | 11000 | 6×50ml | 13480 |
| G16-21 | Angle Rotor | 12000 | 6×70ml | 10810 |
| G16-22.1 | Angle Rotor | 12000 | 4x100ml | 14850 |
| G16-22 | Angle Rotor | 8000 | 6×100ml | 7280 |
| G16-23 | Angle Rotor | 12000 | 24 pieces capillary vessel | 15800 |
| G16-24 | Microplate rotor | 4000 | 2×3×48 Well | 2300 |
The future of lab automation is here.
Opentrons Flex is a liquid-handling robot designed for high throughput and complex workflows. The Flex robot is the base of a modular system that includes pipettes, a labware gripper, on-deck modules, and labware — all of which you can swap out yourself. Flex is designed with a touchscreen so you can work with it directly at the lab bench, or you can control it from across your lab with the Opentrons App or our open-source APIs.
The future of lab automation is here.
Opentrons Flex is a liquid-handling robot designed for high throughput and complex workflows. The Flex robot is the base of a modular system that includes pipettes, a labware gripper, on-deck modules, and labware — all of which you can swap out yourself. Flex is designed with a touchscreen so you can work with it directly at the lab bench, or you can control it from across your lab with the Opentrons App or our open-source APIs.
The PCR-free NGS DNA Library Prep Kit was developed for construction of DNA libraries for next generation sequencing (illumina platform). The kit adds 3′-dT-tailed library adapters to both ends of DNA fragments efficiently. The kit uses double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA fragmentation enzymes etc.) and physical methods (sonication, nebulization etc.).
PCR-Free NGS DNA Library Prep Kit Workflow
PCR amplification is a standard step for library preparation of Next-Generation Sequencing. The PCR is used to amplify fully ligated DNA fragments and to add index information to the libraries. The indexing is for the pooling of the library samples in an effort to reduce the sequencing cost.
However, PCR introduces uneven amplification of DNA in some DNA regions with extreme GC-contents and secondary structures. This bias can cause very low sequencing coverage in such regions. DNA sequencing in these regions is still a huge challenge.
PCR-free library prep can reduce library bias and minimize sequencing gaps. The sequencing data from PCR-free library samples have even genomic coverage with few gaps and better depth in GC-rich regions. Our PCR-free NGS kit offers optimal coverage in the regions that are traditionally difficult, such as high-GC content regions, low-GC content regions, and repetitive sequence regions.
Two index types are available for the kit:
Non-index: Libraries do not have index.
Index: Each library contains one i5 index and one i7 index. Library multiplexing up to 96 samples is possible. List of indexes can be downloaded Here.
Kit advantages:
The PCR-free NGS DNA Library Prep Kit was developed for construction of DNA libraries for next generation sequencing (illumina platform). The kit adds 3′-dT-tailed library adapters to both ends of DNA fragments efficiently. The kit uses double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (BioDynami DNA fragmentation enzymes etc.) and physical methods (sonication, nebulization etc.).
