DBCO-PEG5-DBCO is a bifunctional click chemistry reagent. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
DBCO-PEG5-DBCO is a bifunctional click chemistry reagent. DBCO will react with azide-bearing compounds or biomolecules to form a stable triazole linkage without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
HCM197 Half Fraser Broth
Product Info
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Product Info
Introduction
Intended Use
For the isolation of Listeria spp. from food and environmental specimens.
Principle and Interpretation
Tryptone, proteose peptone, meat extract, yeast paste powder provide nitrogen sources, vitamins, amino acids, and growth factors; sodium chloride can maintain a balanced osmotic pressure; sodium dihydrogen phosphate and potassium dihydrogen phosphate as buffering agents; aesculin are fermentable sugars; lithium chloride and other antibiotics can inhibit Gram negative bacteria and most Gram positive bacteria.
Formulation
Ingredients
/liter
Enzymatic Digest of Animal Tissues
5.0g
Enzymatic Digest of Casein
5.0g
Meat extract
5.0g
Yeast extract
5.0g
Sodium chloride
20.0g
Disodium hydrogen phosphate dihydrate
12.0g
Potassium dihydrogen phosphate
1.35g
Aesculin
1.0g
Lithium chloride
3.0g
pH7.2±0.2 at 25°C
Preparation
Dissolve 57.4g in 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes. Then cool to 50°C,and add one Supplement(SR0120) per 225mL of base to prepare Half-Fraser Broth.
Quality Control
Cultural characteristics observed after incubation at 29-31°C for 22~26 hours
Quality control strains
Growth
Characteristics
Listeria monocytogenes ATCC19115
>20 cfu On PALCAM
Gray to black colony count with black halo
Escherichia coli ATCC25922
< 100 colonies on TSA
Inhibition
Enterococcus faecalis ATCC29212
Storage and Shelf Life
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the isolation of Listeria spp. from food and environmental specimens. Principle and Interpretation Tryptone, proteose peptone, meat extract, yeast paste powder provide nit……
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
Parameters
Details
Product Name
DNA Isothermal Amplification Kit NFO
Manufacturer
Amp-future
Storage Temperature
-20°C
Kit Components
Enzymes, Buffers ,Reagents
Packaging
48 Tests/box
Detection Limit
500-1000copies/µL
Shipping
ICE
Test Time
5-20mins
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
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Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
[NS1000] FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500 μl
Product Info
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Product Info
Description
FluoroVue™ Nucleic Acid Gel Stain (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard to its users. It is a fluorescent stain which offers highly sensitive detection of double-stranded or single-stranded DNA and RNA in a convenient manner. FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity that is several times greater than EtBr.
FluoroVue™ Nucleic Acid Gel Stain is compatible with both conventional UV gel-illumination systems as well as harmless long wavelength blue light illumination systems, like B-BOX™. When bound to nucleic acids, FluoroVue™ Nucleic Acid Gel Stain has a fluorescent excitation maximum of 250 and 482 nm, and an emission maximum of 509 nm. Therefore, it can replace EtBr without the need of changing existing lab imaging systems.
Features:
Excellent for in-gel staining
Sensitivity: 0.14 ng (DNA) or 1 ng (total RNA)
A safer alternative to EtBr
Compatibility: suitable to blue or UV light
Increased cloning efficiency (blue light)
Storage
Protected from light 4°C for 12 months -20°C for 24 months
Document
FluoroVue™ Nucleic Acid Gel Stain (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard to its users. It is a fluorescent stain which offers highly sensitive detection of double-stranded or single-stranded DNA and RNA in a convenient manner. FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity that is several times greater than EtBr.
FluoroVue™ Nucleic Acid Gel Stain is compatible with both conventional UV gel-illumination systems as well as harmless long wavelength blue light illumination systems, like B-BOX™. When bound to nucleic acids, FluoroVue™ Nucleic Acid Gel Stain has a fluorescent excitation maximum of 250 and 482 nm, and an emission maximum of 509 nm. Therefore, it can replace EtBr without the need of changing existing lab imaging systems.