DBCO-PEG5-DBCO

K-GLULQR

SKU: 700007404

50 assays (manual) / 500 assays (auto-analyzer)

Content:	50 assays (manual) / 500 assays (auto-analyzer)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels

Analyte:	D-Glucose
Assay Format:	Spectrophotometer, Auto-analyzer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	1 to 150 µg of D-glucose per assay
Limit of Detection:	2 mg/L
Limit of Quantification:	6 mg/L
Reproducibility (%):	< 5%
Reaction Time (min):	~ 5 min

The D-Glucose (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of D-Glucose in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.

Browse more of our monosaccharide and oligosaccharide assay kits.

Advantages

• “Ready to use” liquid stable formulation – no reconstitution needed
• Rapid reaction (~ 5 min)
• PVP incorporated to prevent tannin inhibition
• Standard included
• Suitable for manual and auto-analyzer formats
• Quick Reference Guide available
• Mega-Calc™ software tool is available for hassle-free raw data processing 

The D-Glucose (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of D-Glucose in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.

Overview

• Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more
• Simple and quick workflow: library could be prepared in less than 5 hours
• Component of Norgen’s metagenomics workflow
• A single NGS run can be prepared with up to 384 unique dual-index libraries

Sample type purification kit guide 

The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.

The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.

Details

Supporting Data

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Minimum amount of starting material:	2.5 µL of DNA (5 ng/µL)
Time to complete library preparation:	4 hours

Storage Conditions and Product Stability
Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.

All kit components should remain stable for at least 1 year when stored at the specified storage conditions.

Step	Component	Cat. 70200 (24 preps)	Cat. 70210, 70220, 70230, 70240 (96 preps)
Amplicon PCR (PCR 1)	MGX Master Mix	330 µL	1,320 µL
	16S V1-V3 Primer Mix	70 µL	280 µL
Index PCR (PCR 2)	Indexing Master Mix	660 µL	2 x 1,320 µL
	N7xx Index Primer	50 µL	50 µL
	S5xx Index Primer	70 µL	70 µL
PCR Clean-Up	Resuspension Buffer	2 x 1,250 µL	2 x 5,000 µL
	Nuclease-free water	1,250 µL	1 x 6,000 µL

K-GALM

SKU: 700004289

100 Assays per kit

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Galactomannan
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Limit of Detection:	1 g/100 g
Total Assay Time:	~ 90 min
Application examples:	Seeds, milling fractions and food ingredients.
Method recognition:	Novel method

The Galactomannon test kit is suitable for the measurement and analysis of Galactomannan in food and plant products.

Browse more of our polysaccharide test kit products.

Advantages

• Galactose dehydrogenase now included in the kit 
• Very cost effective 
• All reagents stable for > 2 years after preparation 
• Only enzymatic kit available 
• Simple format 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Galactomannon test kit is suitable for the measurement and analysis of Galactomannan in food and plant products.