Propargyl-PEG7-alcohol is a propargyl linker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG7-alcohol is a propargyl linker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG7-alcohol is a propargyl linker that can react with azide compounds or biomolecules via copper catalyzed Click Chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Cerillo’s Stratus Plate Reader is a third party module that can be placed next to or inside the OT-2 or Opentrons Flex for post experimental analysis. The worktable adapter will allow you to utuilize the OT-2 or Opentrons Flex to prepare the samples directly into the plate reader positions on the worktable.
This small portable device is primarily for microbial growth curve analysis. The Stratus is compatible with Cerillo’s Canopy wireless device that allows real time visualization, control, and analytics. This product is compatible with reading 600nm wavelengths.
Cerillo’s Stratus Plate Reader is a third party module that can be placed next to or inside the OT-2 or Opentrons Flex for post experimental analysis. The worktable adapter will allow you to utuilize the OT-2 or Opentrons Flex to prepare the samples directly into the plate reader positions on the worktable.
This small portable device is primarily for microbial growth curve analysis. The Stratus is compatible with Cerillo’s Canopy wireless device that allows real time visualization, control, and analytics. This product is compatible with reading 600nm wavelengths.
These kits provide everything required to isolate and purify inclusion body proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilization Reagent. Inclusion body proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting. This kit provides a convenient way to screen recombinants prior to scaling up.
ProteoSpin™ Inclusion Body Protein Isolation Micro Kit
The process is efficient and streamlined and can process up to 12 samples in only 60 minutes. Each spin column is able to recover up to 50 µg of acidic or basic proteins. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.
ProteoSpin™ Inclusion Body Protein Isolation Maxi Kit
The procedure is efficient and streamlined and can process up to 4 samples in approximately 2 hours. Each spin column is able to recover up to 12 mg of acidic or basic proteins from 100 mL of induced bacterial culture. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.
About Inclusion Bodies
Bacteria are widely used for the expression of different proteins. However, 70-80% of the proteins expressed in bacteria by recombinant techniques are typically contained in insoluble inclusion bodies (i.e., protein aggregates). The protein of interest found in these subcellular structures is often inactive, due to incorrect folding. The production rate of recombinant proteins stored in inclusion bodies is invariably higher than those synthesized as soluble proteins. The reason behind this is thought to be the resistance of insoluble proteins to proteolysis by cellular enzymes. In addition, separation of insoluble recombinant proteins in inclusion bodies is considerably easier than that of soluble proteins. These factors have been the major influences favoring scale-up of high-value proteins using bacterial fermentation for example. Procedures for the purification of the expressed proteins from inclusion bodies are often labour-intensive, time-consuming and not cost-effective. This kit provides the essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography – all optimized to work together thereby simplifying the process and saving a tremendous amount of time and cost.
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| Kit Specifications | |
| Maximum Culture Volume | 1.5 mL |
| Yield from 1.5 mL | Up to 50 μg |
| Minimum Elution Volume | 30 μL |
| Time to Process 12 Samples | 60 minutes |
Storage Conditions
The Cell Lysis Reagent and IB Solubilization Reagent should be stored at 4°C upon receipt of this kit. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. Once opened, the solutions should be stored at 4°C when not in use except for the pH Binding Buffers (Acidic and Basic). Some precipitation will occur with 4°C storage. This precipitation should be dissolved with slight heating to room temperature before using.
| Component | Cat. 10300 (Micro – 25 preps) | Cat. 17700 (Maxi – 4 preps) |
|---|---|---|
| Wash Solution C | 30 mL | 130 mL |
| Wash Solution N | 30 mL | 130 mL |
| Binding BUffer A | 4 mL | 20 mL |
| Binding Buffer N | 4 mL | 20 mL |
| Elution Buffer C | 8 mL | 2 x 30 mL |
| Protein Neutralizer | 4 mL | 4 mL |
| Cell Lysis Reagent | 15 mL | 110 mL |
| IB Solubilization Reagent | 2 mL | 50 mL |
| Syringes, 1cc, slip tip | 25 | – |
| Needles (Bev, 20G x 1 inch) | 25 | – |
| Syringes, 10 mL, Luer-Lok™ Tip | – | 4 |
| Needles (18G x 1.5 inch) | – | 4 |
| Micro Spin Columns | 25 | – |
| Maxi Spin Columns (filled with SiC) inserted into 50 mL collection tubes | – | 4 |
| Collection Tubes | 25 | – |
| Elution Tubes (1.7 mL) | 25 | – |
| Elution Tubes (50 mL) | – | 4 |
| Product Insert | 1 | 1 |
These kits provide fast and reliable procedures for the purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.
Fungi/Yeast Genomic DNA Isolation Kit (Spin Column)
This kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and bacteria including Gram-positive. Preparation time for a single sample is less then 30 minutes, and each kit contains sufficient materials for 50 preparations.
Fungi/Yeast Genomic DNA Isolation 96-Well Kit (HT)
Norgen’s Fungi/Yeast Genomic DNA Isolation 96-Well Kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from viable yeast cells, fungal spores or mycelium and Gram-positive bacteria. The purification could be performed on either a vacuum manifold or using centrifugation. Complete 96 purifications in 40 minutes.
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| Kit Specifications | |
| Column Binding Capacity | 50 μg |
| Maximum Column Loading Volume | 650 μL |
| Maximum Amount of Starting Material: Fungi (wet weight) Yeast or Bacteria Culture | 50 mg 0.5mL – 1mL |
| Average Yield* Pichia sp. (yeast) Botrytis cinerea Fusarium sp. Aspergillis niger Mucor racemosus Cladosporium cladosporioides Penicillium sp. Rhizopus oryzae Alternaria tenuissima | 25 μg 32 μg 42 μg 26 μg 15 μg 12 μg 40 μg 7 μg 30 μg |
| Time to Complete 10 purifications | 30 minutes |
* Yield will vary depending on the type of sample processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 27300 (50 preps) | Cat. 27350 (192 preps) |
|---|---|---|
| Lysis Buffer L | 30 mL | 2 x 60 mL |
| Resuspension Solution A | 20 mL | 60 mL |
| Solution BX | 28 mL | 2 x 28 mL |
| Wash Solution A | 18 mL | 2 x 38 mL |
| Elution Buffer B | 15 mL | 30 mL |
| Bead Tubes | 50 | 200 |
| Spin Columns | 50 | – |
| Collection Tubes | 50 | – |
| 96-Well Plate | – | 2 |
| 96-Well Collection Plate | – | 2 |
| Adhesive Tape | – | 4 |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Elution Plate | – | 2 |
| Product Insert | 1 | 1 |