Bacterial DNA Extraction Kit (Magnetic Beads)

The Bacterial DNA Extraction Kit (Magnetic Beads) was developed for the bacterial genomic DNA extraction from bacterial cultures directly using magnetic beads from a wide variety of gram-negative and gram-positive bacterial species, as well as yeast and other fungi. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality bacterial DNA isolation with fast magnetic response time and high binding capacity.

Bacteria are the most diverse and abundant small single-celled organisms and are vital to the planet’s ecosystems. Some bacterial species are pathogens that can cause a variety of diseases to humans and animals. Besides their ecological and biomedical importance, bacteria are also used in biotech and pharmaceutical applications such as production of enzymes, DNA preparation, biofuels, food research, and chemical production.

Bacterial cells are grown to log-phase and the culture is lysed directly with a lysis buffer, then mixed with beads to bind genomic DNA. After wash steps, genomic DNA is eluted in Low TE or TE Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities. Bacterial DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, Southern Blotting, molecular cloning, DNA hybridization, restriction enzymatic digestion, and Next-generation Sequencing (NGS) etc.

Features

• 100% centrifuge-free
  • Bacterial cultures can be used directly without centrifuge to pellet the bacteria
• Simple magnetic beads method
  • No centrifuge needed
  • No column needed
  • No vacuum needed

Purified genomic DNA from various bacteria were isolated with the Bacterial DNA Extraction Kit. A portion of the extracted genomic DNA was loaded on a 1% agarose gel. DNA ladder: BioDynami 1 kb Plus DNA Ladder (Cat.# 10005L).

DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.

DBCO-PEG4-C3-sulfonic acid serves as a bifunctional PEG linker. The DBCO click chemistry handle conjugates with azides on target molecules to form stable triazole linkages. The sulfonic acid can participate in esterification, halogenation and displacement reactions.