Introduction

Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from bacteria, yeast cells
Applications	RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Bacteria, yeast cells
Sample amount	Bacteria: <10^9；Yeast cells：<2 x10^7
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

• Fast – several samples can be extracted in 30 minutes
• High purity – the purified RNA can be directly used in various downstream applications
• High recovery – RNA can be recovered at the level of PG
• Good repeatability – silica gel column purification technology can obtain ideal results every time
• Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
• Sufficient components – the kit contains carried wall breaking enzymes and glass beads

Kit Contents

Contents	R418202	R418203
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	250
Glass Beads (0.1-0.6mm)	30 g	150 g
DNase I	600 μl	5 x 600 μl
DNase Buffer	6 ml	30 ml
Protease Dissolve Buffer	1.8 ml	15 ml
Buffer ATL	50 ml	200 ml
Buffer PHC	50 ml	200 ml
Buffer GXP2*	20 ml	100 ml
Buffer RW1	50 ml	250 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability

Buffer PHC should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Buffer PHC up to 12 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components can be stored at roomtemperature (15–25°C) and are stable for at least 18 months under these conditions.

Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

K-MANGL

SKU: 700004316

55 assays per kit

Content:	55 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	D-Fructose, D-Glucose, D-Mannose
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 µg of D-glucose, D-fructose or D-mannose per assay
Limit of Detection:	~ 0.7 mg/L
Reaction Time (min):	~ 30 min
Application examples:	Foodstuffs, yeast cell preparations, enzymatic hydrolysates and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Novel method

The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

See our full range of monosaccharide and disaccharide assay kits.

Advantages

• Very competitive price (cost per test) 
• All reagents stable for > 2 years after preparation 
• Only enzymatic kit available 
• Simple format 
• Rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The D-Mannose/D-Fructose/D-Glucose test kit is suitable for the specific measurement and analysis of D-mannose, D-fructose and D-glucose in plant products and in acid hydrolysates of polysaccharides.

* Optical system: equipped with infinite flat field optical system, the whole machine mildew prevention design and large field eyepiece, with beautiful appearance, clear imaging, broad vision, convenient operation and other characteristics, can be widely used in biology, medicine, industry, agriculture and other fields, is the ideal instrument for medical, teaching, scientific research and other units

* Observation head device: the cylinder can rotate 360 degrees, the displacement of the center of the mirror is 0.06mm; 30 tilt; the hinge type binocular observation (the magnification difference between left and right systems is 2.0%; the end position difference is 0.1mm at zero vision) and the third party connection port, the pupil distance adjustment range is 50-75mm, the fixed observation cylinder head is internal hexagon screw or select portable screw.

* Eyepiece configuration: random eyepiece WF10X / 22mm; the magnification accuracy of eyepiece shall not exceed ± 2%. There is a card slot on the eyepiece tube to lock the eyepiece to prevent falling. Binocular vision is adjustable, making binocular observation easier;

* Objective: Broadband coated ED-N195 pure copper infinite remote flat field achromatic objective; objective magnification accuracy not exceeding ± 5%; 4X, 10X, 40X (spring), 100X (spring, oil); objective error 10-40 Aim 0.06mm; 40-100 Aim 0.03mm

* Loading platform: triangular rail XY composite mechanical moving loading platform; 0.005 lateral to 5N horizontal force, corrosion resistance and wear resistance. Rectangular, size 190X140mm, stroke 6090mm, minimum read value 0.1 mm.

Parameter of microscope

Specification configuration	TL36A	Binocular observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm
	TL36B	Three-eye observation head, 45° tilt, 360 degrees can be rotated, pupil distance can be adjusted, adjustment range 50-75mm.
	TL36M	Three-eye viewing head with 6.3 megapixel imaging system CMOS camera
	TL36P	Three-eye viewing head, equipped with 10.1 inch integrated flat panel display
Eyepiece	Wide-angle eyepiece WF10X,field of view 22mm,optional WF16X,WF25X
Converter	Quadruple Revolving converter
0bjective	Flat field achromatic objective at infinite distance,4X,10X,40X,100X(oil)
Condenser	ABBE N.A.1.25Condenser with Iris Diaphragm &Filter
Stage	Double Layer Mechanical Stage,  Size1190X140mm,              Moving Range : 60mmx90mm
Focusing	0.02mmCrude motion, micromotion and coaxial focal adjustment system with a minimum focal range of 0.02mm Coarse and Fine Focusing
Light source	3W LED,with Rechargeable Battery,Type C USB cable
Attachments	Dust bag,Type C power cord

TL 36 series biological microscopes are equipped with infinite distance flat field achromatic objective and large field eyepiece, with the characteristics of beautiful appearance, clear imaging, broad vision, convenient operation and so on, can be widely used in biology, medicine, industry, agriculture and other fields, is the ideal instrument for medical, teaching, scientific research and other units