DBCO-S-S-acid is a cleavable reagent for introduction of a carboxylic acid moiety to azide-containing biomolecules using copper-free Click Chemistry. PEG spacer arm provides better solubility to the labeled molecules in aqueous media. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.
DBCO-S-S-acid is a cleavable reagent for introduction of a carboxylic acid moiety to azide-containing biomolecules using copper-free Click Chemistry. PEG spacer arm provides better solubility to the labeled molecules in aqueous media. The disulfide bond in this linker can be cleaved using reducing agents such as DTT, BME and TCEP.
Salmonella enterica have emerged as significant foodborne pathogens that pose a serious public health problem. The symptoms of salmonellosis may include diarrhea, fever, vomiting, and abdominal cramps with elderly, new-born, and immunocompromised individuals the most susceptible. S. enterica is a facultatively anaerobic Gram-negative bacterium that could survive low temperatures and freezing. The majority of the 1.3 billion annual cases of Salmonella-caused human gastroenteritis result from ingestion of contaminated food products, such as raw or undercooked meat, seafood, and eggs, as well as raw or unpasteurized milk and dairy products. Salmonella infections are also contracted following consumption of fresh fruits or vegetables that have been contaminated by infected fertilizer.
Salmonella enterica TaqMan PCR Kit, 100 reactions
Salmonella enterica TaqMan PCR Probe/Primer Set and Controls, 100 reactions
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM32150 (100 preps) | Cat. TM32110 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| S. enterica Primer & Probe Mix | 280 μL | 280 μL |
| S. enterica Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
1. Urine Preservative Single Dose Ampules
With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.
2. Urine Collection and Preservation Tubes
Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:
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Select Urine Tested with the Norgen Urine Collection and Preservation Tubes
Human
Mouse
Lynx
Wolf
Urine collected from snow
Available Sizes and Formats
Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
| Kit Components | Cat. 18118 (50 tubes) | Cat. 18122 (50 tubes) | Cat. 18113 (50 tubes) | Cat. 18126 (50 tubes) | Cat. 18129 (1 cup) |
|---|---|---|---|---|---|
| Urine Collection and Preservation Tubes (5 cc) | 50 | – | – | – | – |
| Urine Collection and Preservation Tubes (15 cc) | – | 50 | – | – | – |
| Urine Collection and Preservation Tubes (50 cc) | – | – | 50 | – | – |
| Urine Preservative Single Dose | – | – | – | 50 | – |
| Urine Collection and Preservation Cup (120 cc) | – | – | – | – | 1 |
| ID Labels | 50 | – | – | 50 | – |
| Product Insert | 1 | 1 | 1 | 1 | 1 |
Product Details
Sample Type:
Serum, Plasma, Whole Blood
Package Size:
48 Tests/Kit
Test Time:
5-20 Minutes
Target Disease:
African Swine Fever Virus
Product Name:
African Swine Fever Virus Isothermal Detetction Fluorescence Kit
Test Principle:
Nucleic Acid Detection
Target Species:
Pigs
High Light:
,
,
Product Description
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Product parameters:
| Kit composition (WLRE8208KIT 48T/Pack) | |
| Composition | Content |
| E buffer | 1 mL×2 Tubes |
| B buffer | 150 μL×1Tube |
| Positive control template | 100 μL×1Tube |
| Reagents | 48T |
Product features and advantages:
| Product features and advantages | |
| Features | Advantages |
| High sensitivity | 1 copies/ml |
| Strong specificity | Specificity is superior to PCR |
| Short reaction time | 20min |
| Polymorphic reagent | Liquid, freeze-dried powder, freeze-dried ball |
| Easy to operate | Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple |
| Easy to store and transport | It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation |
| Low requirements on equipment | Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector |
Product application:
| Application | |
| Ultra-clean laboratory | African Swine Fever Virus Isothermal Detection |
| Indoor | |
Operation procedure and process:
| Operation procedure and process: |
| Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix. |
| Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent. |
| Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid |
| Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control |
| Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap) |
| Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone. |
Performence Comparison Data:
| No. | TT value | Template concentration | Result determination |
| 1 | 4.2 | 10-1 | Positive |
| 2 | 5.5 | 10-2 | Positive |
| 3 | 10.2 | 10-3 | Positive |
| 4 | 17.0 | 10-4 | Positive |
| 5 | 12.0 | 10-5 | Positive |
| 6 | – | 10-6 | Negative |
| 7 | – | 10-7 | Negative |
| 8 | Negative control | Negative | |
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
