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These DNA polymerases are ideal for Isothermal Amplification

IsoPol® BST⁺ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST⁺ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.

Key features

• IsoPol® BST⁺ lacks 5’-3’- and 3’-5’-exonuclease activity.
• Inactivation – IsoPol® BST⁺ is completely inactivated after one minute incubation at 95°C
• Enhanced strand displacement and processivity.
• Increased salt tolerance.

IsoPol® BST⁺ Glycerol FREE 

Is the popular choice f or isothermal applications such as LAMP and RT-LAMP at point-of-care diagnostics for its superior amplification performance and robustness. The enzyme is offered in a 1X storage buffer at a concentration < 300 U/µl

IsoPol® BST⁺ High Concentration Glycerol FREE

(IsoPol® BST⁺ HC Glycerol FREE) is used to ease the product development processes in sequencing technologies, solid phase amplification and several isothermal technologies. Same performance as IsoPol® BST⁺ (i.e., high processivity, relative SDA, and relative activity) with the only difference that it is offered in a 2X storage buffer at a concentration > 500 U/

Key features

• High concentration
• High purity
• Lyophilisation and automation friendly

Figures

Properties

These DNA polymerases are ideal for Isothermal Amplification

IsoPol® BST+ is a heat-tolerant BST polymerase (large fragment) with enhanced strand-displacement activity. IsoPol® BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.

Introduction

HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Purification and concentration of RNA（mRNA>200nt or miRNA）from transcription products, DNase cleavage products, labeled products, and crude RNA products
Applications	Sequencing, ligation, enzyme digestion, RT-PCR, labeling, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Crude RNA, enzymatic reaction solution
Sample amount	≤100μl
Recovery	90%
Elution volume	≥15μl
Time per run	≤15 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Binding buffer is added directly to the sample or other enzymatic reaction, and the mixture is applied to the column. Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure RNA is eluted with a small volume of low-salt buffer provided or water, ready to use in all subsequent applications.

Advantages

• High recovery – recovery of RNA up to 90%
• Low elution volume – the elution volume can be as low as 15µl
• Fast – only 10 minutes for recovery by using column method
• General – suitable for various crude RNA products
• Wide range of fragment recovery:(>25nt RNA)

Kit Contents

Contents	R214402	R214403
Purification Times	50 Preps	250 Preps
Buffer GXP	30 ml	120 ml
Buffer RW2	20 ml	2 x 50 ml
RNase-Free Water	20 ml	60 ml
HiPure RNA Mini Columns I	50	250
2 ml Collection Tubes	50	250

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

HiPure RNA Clean Up Kit provides a rapid and easy method for the purification and concentrate RNA from enzymatic reactions or for desalting the RNA samples. RNA purified using HiPure RNA Clean Up Kit is ready for all downstream applications such as RT-PCR, Northern blotting, mRNA purification, nuclease protection, and in vitro translation.

Overview

• All shipping requirements in one convenient mailer
• Leak-proof biohazard bags with adsorbent pads for enhanced safety
• Matches the standards of OSHA as well as the IATA regulations

Norgen’s Shipping Accessories provides a safe and convenient tool for shipping non-infectious biological specimens. The accessories are contained in individual SHIP TO envelopes that are used for shipping of both the accessories as well as the individual sample collection device to the donor/user.

Each SHIP TO envelope contains:

1. A Biohazard Specimen Bag with absorbent pad;
2. A Bubble Envelope labeled as “Exempt Human Specimen”;
3. 2 blank labels; 
4. Shipping Instructions

The Biohazard Specimen Bag is a sure-seal zipper bag to hold the collected specimen and protect against accidental spills. The bag contains an absorbent pad that can adsorb up to 4 mL of liquid and meets current labeling requirements of OSHA standard 29CFR1910.1030 regarding biohazard symbols. The Bubble Envelope is used as a RETURN envelope for the donor/user to ship the collected sample back to the lab for analysis. The user will first place the collected sample into the Biohazard Specimen Bag, and then place the Biohazard Specimen Bag into the Bubble Envelope. The Bubble Envelope protects against moisture and punctures, and facilitates smooth insertion with convenient self-sealing closure. The 2 labels are used for both the outer SHIP TO envelope and the RETURN bubble envelope. The SHIP TO label will be filled out to contain the mailing information of the donor and the RETURN label will be filled out to contain the address to which the sample should be returned. The labels can be supplied as sheets together with the word template file for ease of printing. Norgen’s Shipping Accessories match the regulations of the International Air Transport Association (IATA).

Details

Shipping Accessories Contents
SHIP TO Envelopes	50
Product Insert	1
SHIP TO Envelope Contents
Biohazard Specimen Bag with absorbent pad	1
Bubble Envelope	1
Labels	2
Shipping Instructions	1