Cell membrane preparations containing nicotinic AChRs that are ligand-gated ion channels that form pores in cells plasma membranes, mediating fast signal transmission at synapses. Nicotinic AChRs are involved in a wide range of physiological processes, and can be either neuronal or muscle-type. The membrane preparations we have developed are suitable for receptor binding assays in which muscle type nicotinic AChRs are needed. The membranes are tested in several functional binding assays and quality testing criteria to meet binding specifications.
Other Products
Opentrons Flex Filter Tips, 50 µL
Product Info
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Product Info
50 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
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50 µL filtered pipette tips for the Opentrons Flex, provided sterile in racks or blister packs (refills). Tips are clear, non-conducting polypropylene tips, free of human DNA, PCR inhibitors, RNase/DNase, pyrogens, and endotoxins. Opentrons Flex Tips and Racks are autoclavable.
This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from bacterial culture
Applications
PCR, southern blot and enzyme digestion, etc.
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Gram negative or positive bacteria
Sample amount
Bacterial culture: 0.5-1.5ml
Elution volume
≥60μl
Time per run
≤60 minutes
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
Fast – several samples can be extracted in 60 minutes (after digestion)
High purity – unique adsorbent can completely remove inhibitory factors
High recovery – DNA can be recovered at the level of PG
Kit Contents
Contents
D513101
D513102
D513103
Purification Times
48 Preps
96 Preps
480 Preps
MagPure Particles
1.2 ml
2.5 ml
11 ml
Lysozyme
60 mg
120 mg
600 mg
Proteinase K
24 mg
50 mg
220 mg
Protease Dissolve Buffer
3 ml
6 ml
30 ml
Buffer SDS
1.5 ml
3 ml
15 ml
Buffer MLA
30 ml
60 ml
300 ml
Buffer GW1*
13 ml
44 ml
110 ml
Buffer TE
30 ml
40 ml
200 ml
Storage and Stability
MagPure Particles, Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
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This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA (e.g., genomic, plasmid) can be purified from bacterial cultures.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Fructan
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
410
Signal Response:
Increase
Linear Range:
2.3 to 55 µg of D-fructose or D-glucose per assay
Limit of Detection:
0.16 g/100 g
Total Assay Time:
~ 90 min
Application examples:
Flours, infant formula, animal feed, pet foods, plant materials (e.g. onion), food products and other materials
Method recognition:
AACC Method 32-32.01, AOAC Method 999.03, AOAC Method 2016.14, AOAC Method 2018.07 and CODEX Method Type III
The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).
New, improved procedure.
In the most recent development, a recombinant endo-levanase has been incorporated into the fructanase mixture, extending the use of the method to the measurement of levan-type fructans as are present in grasses such as timothy, cocksfoot, ryegrass and red fescue.
The method described in this booklet employs ultra-pure, recombinant enzymes and specifically measures fructans including inulin-type fructans from chicory, dahlia, jerusalem artichoke; highly branched fructans from onion and wheat stems and leaves; and levan-type fructans from pasture grasses such as timothy grass. The enzymes employed are completely devoid of contaminating enzymes active on β-glucan or gluco-oligosaccharides.
All kit reagents stable for > 2 years after preparation
Unaffected by high sucrose / reducing sugar concentrations
Fructan kits are only available from Megazyme
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Document
The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).
