Description
The ExcelDye™ 6× DNA Loading Dye (Orange) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration. The Orange G migrates at approximately 30 bp on a standard 2% TAE agarose gel (50 bp on 1% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
- 0.15% Orange G
- 10 mM Tris-HCl (pH 8.0)
- 60% glycerol
- 60 mM EDTA
Storage
4°C for 12 months
-20°C for 36 months
