[DL3000] ExcelDye™ 6X DNA Loading Dye, Blue, 5 ml x 2
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The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Detail
Description
The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
0.03% Xylene cyanol FF
0.03% Bromophenol blue
10 mM Tris-HCl (pH 8.0)
60% glycerol
60 mM EDTA
Storage
4°C for 12 months -20°C for 36 months
Other Products
[CD7000] SMOChem™ dUTP Solution – Sodium Salt (100 mM), 25ml
Product Info
Document
Product Info
Description
Ultrapure dUTP (2´-Deoxyuridine, 5´-Triphosphate) supplied as sodium salt in purified water (pH 8.5). dUTP can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is tested to ensure specific DNA amplification and the absence of nuclease activity.
Features
Ideal for PCR amplification and cDNA synthesis
Nuclease and ribonuclease free
Applications
PCR
Avoid carryover contamination between PCRs to eliminate a source of false positives.
Storage
-20°C for 36 months
Document
Ultrapure dUTP (2´-Deoxyuridine, 5´-Triphosphate) supplied as sodium salt in purified water (pH 8.5). dUTP can be used in place of dTTP in PCR and RT-PCR protocols to prevent carryover from previous amplifications. The substitution of dUTP for dTTP in PCR results in uracil-containing PCR products that are suitable for most standard applications. The enzyme uracil-N-glycosylase (UNG, also referred to as UDG) can be added to a PCR premix to excise uracil from any contaminating PCR product, thereby preventing false positives. Each lot of dUTP is tested to ensure specific DNA amplification and the absence of nuclease activity.
Attogene’ s Cylindrospermopsin Lateral Flow Kit can be used to detect Cylindrospermopsin in source water samples.
Format: Rapid-Water – Run Time: 30 Minutes, enough to run two samples at 10 and 100 fold dilutions and two negative controls.
Cylindrospermopsin (CYN) is a potent cyanotoxin synthesized by select species of cyanobacteria, prominently including Cylindrospermopsin raciborskii. It belongs to the tricyclic alkaloid class, exhibiting a molecular weight of approximately 415 Da. Structurally, cylindrospermopsin features an uracil ring fused with a hydantoin moiety, alongside a guanidino group, attributes that render it highly soluble and polar in aqueous environments.
Cylindrospermopsin is notorious for its profound toxicity towards aquatic organisms and its potential threat to human health through exposure via contaminated water and food sources. Consequently, rigorous monitoring protocols are essential in regions prone to cyanobacterial blooms, where cylindrospermopsin can accumulate in freshwater reservoirs and other aquatic habitats. In recognition of these risks, regulatory bodies such as the United States Environmental Protection Agency (EPA) have implemented an action level guideline. As of 2019, EPA 10-day drinking water health advisory for cylindrospermopsin recommended a threshold of 0.7 parts per billion (ppb), or 700 parts per trillion (ppt) for children under the age of six, and 3 parts per billion, 3000 parts per trillion for anyone older, to effectively manage cylindrospermopsin levels. This precautionary measure aims to uphold both environmental sustainability and public health integrity by minimizing exposure risks. The EPA has also drafted a human health recreational water quality criterion to protect human health at 8,000ppt.
Do not Drink – 0.7 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions
Do not Drink – 3.0 μg/L for school age children to adults
Do Not Use – 20 μg/L
EPA Draft Human Health Recreational Ambient Water Quality Criteria to protect human health: 8 μg/L
Document
Screening of Cylindrospermopsin in water samples at or above 30ppt
Format: 15 tests (5 samples at 2 dilutions/5 controls)
Syringe
Sample Filter
Sample Dilution Buffer
Water Collection Bottle
Water Collection Tube
200ul fixed volume pipette
Water Sample Bottle
Negative control
