[DL3000] ExcelDye™ 6X DNA Loading Dye, Blue, 5 ml x 2
Facebook
X
Pinterest
Email
The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Detail
Description
The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
0.03% Xylene cyanol FF
0.03% Bromophenol blue
10 mM Tris-HCl (pH 8.0)
60% glycerol
60 mM EDTA
Storage
4°C for 12 months -20°C for 36 months
Other Products
[DM4160] FluoroBand™ XL 25 kb Fluorescent DNA Ladder, Broad Range (up to 25 kb), 500 μl
Product Info
Document
Product Info
Description
The DM4160 FluoroBand™ XL 25 kb Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM4160 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Directly observed by UV or blue light— premixed with high sensitive DNA fluorescent dye
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
250 ~ 25,000 bp
Concentration
51.6 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Protected from light Room temperature for 6 months 4°C for 12 months -20°C for 24 months
Document
The DM4160 FluoroBand™ XL 25 kb Fluorescent DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with high sensitivity DNA binding fluorescent dye and loading dye for direct gel loading. The DNA Ladder DM4160 is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA; these bands can be visualized when illuminated with 470 nm blue light or UV light. This product contains two enhanced bands (3 kb and 1 kb) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Real time observation of the electrophoresis is also possible if compatible light source is fitted to the electrophoresis tank.
Simultaneous isolation of both host RNA and microbial RNA (universal protocol)
Isolate full diversity of RNA from large RNA down to small and microRNAs
Eliminates PCR inhibitors including humic acids
High quality RNA for sensitive downstream application
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes for 10 samples.
Free Download
Extracting Biological Insights from Stool
Tips and tricks for isolating high yield and quality DNA, RNA, miRNA and EV’s from fecal samplesDownload for Free
200 mg (fresh/frozen stool) or 400 μL (preserved stool)
Type of Stool Processed
Preserved, fresh, and frozen stool from humans and animals
Maximum Column Binding Capacity
50 μg
Maximum Column Loading Volume
600 μL
Time to Complete 10 Purifications
30 minutes
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
Synovial Fluid Bacterial Genomic DNA Purification Kit
Product Info
Document
Product Info
Overview
Isolate genomic DNA from all types of bacteria (both Gram-positive and Gram-negative)
Rapid and convenient spin column protocol
Purified bacterial gDNA has a minimal host gDNA contamination.
High yield, high quality DNA for sensitive downstream applications including sequencing, PCR, qPCR and more
Synovial fluid is secreted into the joint cavity from the inner membrane of synovial joints. Synovial fluid is a plasma ultrafiltrate which contains proteins derived from both the blood plasma and produced by cells within the joint tissues. It lubricates the articulating joints as well as supplies oxygen and nutrients while removing carbon dioxide and metabolic wastes from the chondrocytes in the surrounding cartilage. Septic arthritis is usually caused by bacterial, viral or fungal infections to the synovial fluid. Staphylococcus aureus is the most common bacterial infection causing Septic arthritis. Diagnosing Septic arthritis is done through joint fluid (synovial fluid) analysis, blood tests or imaging tests.
Norgen’s Synovial Fluid Bacterial Genomic DNA Isolation Kit provides a fast, reliable and simple procedure for isolating the highest quality and the highest quantity of bacterial genomic DNA from various amounts of synovial fluid ranging from 1 mL up to 10 mL. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all Gram +ve and Gram -ve strains. Moreover, the kit allows the user to elute the purified bacterial genomic DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified bacterial gDNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern Blot analysis and NGS.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
