Description
The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.
Composition
- 0.03% Xylene cyanol FF
- 0.03% Bromophenol blue
- 10 mM Tris-HCl (pH 8.0)
- 60% glycerol
- 60 mM EDTA
Storage
4°C for 12 months
-20°C for 36 months
