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[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 ml

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FluoroDye™ DNA Fluorescent Loading Dye is a ready-to-use 6X DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye™ Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye™ DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye™ DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye™ emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.

Detail

Description

FluoroDye™ DNA Fluorescent Loading Dye is a ready-to-use 6X DNA loading dye designed for fast qualitative electrophoresis analysis. Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye™ Fluorescent DNA Loading Dye has negligible background and renders destaining process unnecessary. The FluoroDye™ DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time. FluoroDye™ DNA Fluorescent Loading Dye is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye™ emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm.

Features:

  • Excellent for premix with DNA samples
  • Sensitivity: 0.14 ng (DNA)
  • A safer alternative to EtBr
  • Compatibility: suitable to blue or UV light
  • Increased cloning efficiency (blue light)

Composition 

FluoroDye™ DNA Fluorescent Loading Dye is stored in 6X concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.


Storage

Protected from light
 -20°C for 24 months

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D3144 HiPure Soil/Stool DNA 96 Kit

Introduction

This product allows rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples. Up to 100mg stool sample and 500 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed inparallel.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate
ApplicationsPCR, southern blot and enzyme digestion, etc.
Purification method96 well plate
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeSoil, stool, other environmental samples
Sample amountSoil: 200-500mgStool: 50-100mgOther environmental samples: 100-500mg
Elution volume
Time per run
Liquid carrying volume per column
Binding yield of column


Principle

Soil/Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Advantages

  • Fast – several samples can be extracted in 40 minutes (after digestion)
  • High purity – the purified DNA can be directly used in various downstream applications
  • Good repeatability – silica technology can obtain ideal results every time
  • High recovery – DNA can be recovered at the level of PG

Kit Contents

ContentsD314401D314402D314403
Purification Times1 x 96 Preps4 x 96 Preps20 x 96 Preps
HiPure DNA Plate1420
96 Well Plate (2.2ml)1420
1.6ml Collection Plate1420
0.8ml Collection Plate1420
2ml Bead Tubes1004002000
Buffer SOL100 ml360 ml2 x 900 ml
Buffer SDS10 ml36 ml180 ml
Reagent DX1 ml1.8 ml9 ml
Buffer PS20 ml80 ml400 ml
Absorber Solution20 ml80 ml400 ml
Buffer GDP150 ml500 ml3 x 900 ml
Buffer GW2*50 ml100 ml4 x 200 ml
Buffer AE30 ml120 ml500 ml

Storage and Stability

Absorber Solution should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 200 RXN

Description 

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program. 

With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is also compatible with ROX reference dye if ROX is recommended by the manufacturer of the qPCR system. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

Features

  • High Stability
  • Fast Hot Start
  • High Sensitivity
  • Low Background  
  • Suitable for Fast Program 
  • Smart Blue Contrast Dye 

Storage

Protect from light.
Aliquot to avoid multiple freeze-thaw cycles. 

-20°C for 12 months