[DL5000] FluoroDye™ DNA Fluorescent Loading Dye (Green, 6X), 1 ml

DBCO-PEG2-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG2-acid is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO groups is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain allows for increased water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Adenovirus Purification from any input – cell fraction or media fraction
• Rapid purification within 2 to 4.5 hours
• No specialized equipment needed (ultracentrifuge not required)
• Purify adenovirus cell culture supernatant from 1 mL to 33.5 mL input per prep
• Purify adenovirus cell pellet from 1 mL of input per prep
• Up to 25X sample concentration
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Adenoviral vectors are useful tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenovirus are highly promising for cancer treatment. Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger.  Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. Adenoviral vectors purified in this manner are highly active for use in transduction experiments.

Norgen’s Adenovirus Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. 

Details

Supporting Data

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Kit Specifications
Resin Binding Capacity (total per kit)	At least 3×1011 adenovirus particles as determined by qPCR At least 3×108 transducing units as determined by transduction assay
Input type	Cells, media
Input volume (Supernatant)	1 – 33.5 mL SN per prep (500 mL SN in total)
Input Volume (Cell pellet)	1 mL cell pellet per prep (15 mL in total)
Minimum elution volume	1.3 mL per prep
Time to complete purification	2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction	Yes

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer P should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 67600 (15 preps)
Lysis Buffer S	5.5 mL
HL-SAN Nuclease	102 µL
Binding Buffer A	20 mL
Purification Solution C	60 mL
Purification Solution D	130 mL
Wash Solution C	2 x 130 mL
Slurry E	12.5 mL
Elution Buffer P (store at 4°C)	66 mL
Protein Neutralizer	4 mL
Elution tubes (1.7 mL)	50
Product Insert	1