The DM2100 ExcelBand™ 100 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2100 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Detail
Description
The DM2100 ExcelBand™ 100 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2100 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 1,500 bp
Concentration
52.2 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months 4°C for 12 months -20°C for 36 months
Attogene has developed a set of RNase-Free Lateral Assay Buffers specifically designed for developers of Nucleic Acid-based lateral flow tests
Components
RNase Free Running Buffer Screening Buffer Pack – Five 10 mL bottles of diverse running buffers
Set of 5 RNase-Free Lateral flow running buffers at specifically developed for optimizing nucleic acid based lateral flow assays. Each buffer is certified RNase-Free and tested functionally in RNA and DNA based lateral flow tests.
Features & Benefits
Can be used for development of a lateral flow assay for detection of a nucleic acids.
Cost-effective way to screen multiple buffers for lateral flow assay development knowing they have been functionally tested to be RNase-Free.
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Isolation up to 1.5mg endotoxin-free plasmid DNA from 200ml bacterial culture
Applications
Cell transfection, animal injection, etc.
Purification method
Maxi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Conventional plasmid vector
Sample amount
High copy plasmid vector: 100-150ml culture mediumLow copy plasmid vector: 150-200ml culture medium
Yield
200-1500μg
Elution volume
≥0.7ml
Time per run
≤60 minutes
Liquid carrying volume per column
20ml
Binding yield of column
1mg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – silica gel column purification is much faster than ion exchange
High yield – up to 1.5mg plasmid can be binded in one column
Ultra low endotoxin – content of endotoxin is less than 0.1 EU/μg, which can be directly used for cell transfection and animal injection, etc.
Kit Contents
Contents
P115602
P115603
Purification Times
10 Preps
50 Preps
RNase A
30 mg
150 mg
Buffer P1
100 ml
500 ml
Buffer P2
100 ml
500 ml
Buffer LN3
50 ml
250 ml
Buffer PW1
33 ml
180 ml
Buffer PW2
20 ml
100 ml
Elution Buffer
15 ml
120 ml
Buffer CL
33 ml
180 ml
HiPure DNA Maxi Columns C
10
50
Lysate Clear Midi Syringe
10
50
50 ml Collection Tubes C
20
100
Storage and Stability
The kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers,warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2–8°C.
The HiPure Plasmid EF Maxi Kit combines the power of HiPure technology with Magen’s innovative endotoxin removal technology (ETR) to deliver high-quality plasmid DNA with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Up to 1000 µg high copy number plasmid DNA or 200 µg low copy number plasmid DNA can be purified from 150 mL overnight culture.
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
Product parameters:
Kit composition(WLRE8208KIT 48T/Pack)
Composition
Content
E buffer
1 mL×2 Tubes
B buffer
150 μL×1Tube
Positive control template
100 μL×1Tube
Reagents
48T
Product features and advantages:
Product features and advantages
Features
Advantages
High sensitivity
1 copies/ml
Strong specificity
Specificity is superior to PCR
Short reaction time
20min
Polymorphic reagent
Liquid, freeze-dried powder, freeze-dried ball
Easy to operate
Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the resultsDesign of primers and probe is simple
Easy to store and transport
It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light.The freeze-dried form has a long storage time and is convenient for transportation
Low requirements on equipment
Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument;Applicable to our WL-16-III and other isothermal fluorescence detector
Product application:
Application
Ultra-clean laboratory
African Swine Fever Virus Isothermal Detection
Indoor
Operation procedure and process:
Operation procedure and process:
Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.
Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.
Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid
Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control
Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)
Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.
Performence Comparison Data:
No.
TT value
Template concentration
Result determination
1
4.2
10-1
Positive
2
5.5
10-2
Positive
3
10.2
10-3
Positive
4
17.0
10-4
Positive
5
12.0
10-5
Positive
6
–
10-6
Negative
7
–
10-7
Negative
8
Negative control
Negative
Support and Services:
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
Our technical support staff is available to answer any questions you may have about the product.
We offer online tutorials and user guides to help you understand the product better.
We provide technical assistance to help troubleshoot any issues you may be having with the product.
We offer a warranty on our products.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
FAQ:
Q: What diseases can Livestock Disease Kit detect? A: Livestock Disease Kit can detect different infectious diseases, such as Foot and Mouth Disease, Bluetongue Disease, Bovine Viral Diarrhea.
Q: How does Livestock Disease Kit work? A: Livestock Disease Kit uses advanced MIRA technology to amplify the target DNA/RNA targent sequences , and detect the disease by analyzing the amplified sequences.
Q: How long does it take to get results from Livestock Disease Kit? A: Livestock Disease Kit can give a result within 5-20 mins.
Q: What type of sample is suitable for Livestock Disease Kit? A: Livestock Disease Kit is suitable for blood, serum, and tissue samples.
Q: Does Livestock Disease Kit require professional equipment? A: No, Livestock Disease Kit does not require any professional equipment,can use with our isothermal equipment or pcr machine
Document
Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
