The DM2100 ExcelBand™ 100 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2100 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Detail
Description
The DM2100 ExcelBand™ 100 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA ladder DM2100 is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easier reference. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring.
Features
Sharp bands
Quick reference— enhanced bands
Ready-to-use— premixed with loading dye for direct loading
Stable— room temperature storage over 6 months
Source
Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Range
100 ~ 1,500 bp
Concentration
52.2 µg/ 500 µl
Recommended loading volume
5 µl/ well
Storage
Room temperature for 6 months 4°C for 12 months -20°C for 36 months
Other Products
ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits
Product Info
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Product Info
Overview
Columns bind proteins of interest while endotoxins flow through
Reduce endotoxin levels to less than 0.01 EU/µg of protein
Proteins are desalted
Greater than 95% protein recovery
Concentrate protein samples and remove detergents at the same time
Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl2, NaCl, KCl, CaCl2, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.
Mini Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Maxi Kit
The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.
Including Triton® X-100, CHAPS, NP-40 and Tween 20
Minimum Elution Volume
50 μL
Time to Complete 10 Purifications
20 minutes
Storage Conditions All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment.
Component
Cat. 22800 (25 preps)
Cat. 22200 (4 preps)
Binding Buffer J
8 mL
8 mL
Binding Buffer N
4 mL
20 mL
Wash Solution M
50 mL
130 mL
Wash Solution CIP
20 mL
60 mL
Wash Solution N
30 mL
130 mL
Wash Solution NIP
20 mL
60 mL
Elution Buffer G
6 mL
20 mL
Endotoxin Removal Solution
1.5 mL
1.5 mL
Protein Neutralizer EF
2 mL
2 mL
Maxi Spin Columns (assembled with Collection Tubes)
–
4
Mini Spin Columns
25
–
Collection Tubes
25
–
Maxi Spin Columns (assembled with Collection Tubes)
Lyophilized format designed for room temperature shipping
Available in TaqMan format for analysis
Norgen’s Candida albicans TaqMan PCR Lyophilized Kit is designed for the detection of Candida albicans specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
Norgen’s Candida albicans TaqMan Lyophilized Probe/Primer and Control Set is designed for the detection of Candida albicans specific DNA in a real-time PCR based on the use of TaqMan® technology. The lyophilized format is designed to ship the kit at ambient temperature.
All kit components should be stored at -20°C upon arrival.
Once reconstituted, repeated thawing and freezing (>2 times) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Each kit is provided with 4 tubes of 2X PCR Master Mix and each tube is enough to run 25 reactions. It is not necessary to reconstitute all Master Mix tubes at once. The Master Mix tubes can be reconstituted as and when needed.
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
TGL20 Table Top High Speed Refrigerated Centrifuge
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Product Info
TGL20 Technical Parameters
Max Speed
21000r/min
Max Volume
6×100ml
Timer
1~99h59min
Dimension
570×620×380mm
Speed Accuracy
±20r/min
Max RCF
30910×g
Noise
≤58dBA
Net Weight
84KG
Power supply
AC 220V, 50HZ 10A
Temperature Range
-20℃~40℃
Temperature Accuracy
±1℃
Matched Rotors for TGL20
Order no
Rotor
Max Speed(r/min)
Volume(ml)
Max RCF(*g)
G20-1
Fixed Rotor
16000
4×8PCR
15760
G20-2
Fixed Rotor
15000
6×8PCR
21420
G20-3
Fixed Rotor
16000
8×8PCR
17480
G20-4
Fixed Rotor
15000
12×8PCR
22930
G20-5
Fixed Rotor
21000
12×1.5/2ml
30910
G20-6
Fixed Rotor
15000
40×0.5ml
22920
G20-7
Fixed Rotor
17000
24×1.5/2ml
26460
G20-8
Fixed Rotor
13500
30×1.5/2ml
19340
G20-9
Fixed Rotor
13000
48×1.5/2ml
17930
G20-10
Fixed Rotor
16000
16×5ml
22020
G20-11
Fixed Rotor
16000
6×10ml
21500
G20-12
Fixed Rotor
15000
12×10ml
22680
G20-13
Fixed Rotor
16000
12×7ml
21380
G20-14
Fixed Rotor
13000
16×10ml
19490
G20-15
Fixed Rotor
10000
12×15ml
11840
G20-16
Fixed Rotor
13000
8×15ml(falcon)
17790
G20-17
Fixed Rotor
5000
24×15ml
3500
G20-18
Fixed Rotor
15000
8×20ml
22680
G20-19
Fixed Rotor
5000
30×15ml
3830
G20-20
Fixed Rotor
14000
6×30ml
19060
G20-21
Fixed Rotor
13000
6×50ml(falcon)
18840
G20-22
Fixed Rotor
13000
6×50ml(round)
18730
G20-23
Fixed Rotor
13000
4×85ml
18940
G20-24
Fixed Rotor
12000
6×70ml
15570
G20-25
Fixed Rotor
12000
4×100ml
14850
G20-26
Fixed Rotor
12000
6×100ml
16390
G20-27
Fixed Rotor
12000
24 pieces capillary vessel
15800
G20-28
Fixed Rotor
18000
30×0.5ml
26660
G20-29
Swing Rotor
13000
4×5ml
14960
G20-30
Vertical Rotor
16000
16×5ml
16540
G20-31
Vertical Rotor
14000
8×30ml
16450
G20-32
Microplate Rotor
4000
2×3×48（well）
2300
Document
Features: 1. Digital display indicates the speed,time,temperature and RCF. 2. Brushless DC motor in great torque, free maintenance,no powder pollution.quick in speed up and down. 3. There are many kinds of rotors for choice. 4. Automatically electric lid lock,super speed,over temperature protection and imbalance protection. 5. The centrifuge body is made of high-quality steel,safe and reliable.
