The kit includes BR Dye, BR Dilution Buffer, and two DNA Standards. The assay is accurate for DNA concentrations from 100 pg/µL to 1000 ng/µL, and is highly selective for double-stranded DNA over RNA.
Features
Kit was optimized for use with the Qubit® Fluorometer
Uses the Qubit® dsDNA Broad Range assay setting
Linear range is 2-1000 ng dsDNA
Great saving since the kit cuts the costs by 60%
DNA selectivity and sensitivity. A series of input DNA (200, 400, 600, 800, and 1000 ng) was used with or without RNA contamination.
Other Products
Propargyl-PEG4-(CH2)3-acid
Product Info
Document
Product Info
Propargyl-PEG4-(CH2)3-CO2H is a linker containing a propargyl group at one end and a carboxylic acid at the other end. The carboxylic acid reacts with amine groups in the presence of activators (EDC or HATU). Under the catalyzation of copper, the propargyl group forms linkage with azide group of biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
Propargyl-PEG4-(CH2)3-CO2H is a linker containing a propargyl group at one end and a carboxylic acid at the other end. The carboxylic acid reacts with amine groups in the presence of activators (EDC or HATU). Under the catalyzation of copper, the propargyl group forms linkage with azide group of biomolecules. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 1-100ul blood, FFPE, tissue and other samples
Applications
Second generation sequencing, PCR, real timePCR, etc.
Purification method
Monodisperse magnetic beads
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells
Sample amount
Body fluid : 10 – 200μl, Tissue : ≤10mg
Yield
10ng – 15μg
Elution volume
80 -100μl
Time per run
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
High binding force – suitable for handling DNA rich samples, such as whole blood, buffy coat, concentrated blood, etc.
High purity – carboxyl magnetic beads with weak surface adsorption, getting higher purity
General – can be used for various clinical samples
Kit Contents
Contents
IVD3101
Purification Times
200
MagBind Particles
4.5 ml
Proteinase K
90 mg
Protease Dissolve Buffer
10 ml
Buffer ATL
60 ml
Buffer AL
60 ml
Buffer BD*
20 ml
Buffer BXW1*
110 ml
Elution Buffer
30 ml
Storage and Stability
Proteinase K, MagBind Particles should be stored at 2–8°C upon arrival. However, short-termstorage (up to 18 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.
