The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons
Detail
Name of Product
HybriDetect – Universal Lateral Flow Assay Kit
Catalog Number
MGHD 1
Short Info
The HybriDetect is a simple and quick tool to develop your own rapid test. Various molecules can be detected: Proteins, Antibodies, Genetic amplicons
Please note, that you may have to pay country-specific taxes and duties.
Method/Platform
Lateral flow, sandwich or competetive
Range/Assay Sensivity
5 pg DNA, eqivalent to agarose gel electrophoresis
Test Principle
HybriDetect is a ready-to-use, universal test strip (dipstick) based on lateral flow technology using gold particles. The dipstick can be used to develop qualitative or quantitative rapid test systems for a wide range of analytes such as antibodies, gene amplification products or proteins . The results can be interpreted qualitative or quantitative.
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Product Info
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Product Info
We will help to establish your experiments using our broad knowledge:
With myPOLS Biotec’s access to large libraries of different mutant polymerases and experience in high throughput screening we can find the best enzyme for your needs.
We also optimize your polymerases for your defined requirements or enhance our DNA polymerase-based products accordingly.
Finally, we can develop freeze-dried enzyme formulations and production.
We at myPOLS Biotec are scientists and have vast experience in polymerase-based product development. You can benefit from this. Get in touch.
Document
We will help to establish your experiments using our broad knowledge:
With myPOLS Biotec’s access to large libraries of different mutant polymerases and experience in high throughput screening we can find the best enzyme for your needs. We also optimize your polymerases for your defined requirements or enhance our DNA polymerase-based products accordingly. Finally, we can develop freeze-dried enzyme formulations and production.
The 16S V1-V3 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V1-V3 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others.
The 16S V1-V3 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below). Input DNA is first subjected to targeted PCR to amplify the V1-V3 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5′ and 3′ barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
Storage Conditions and Product Stability Norgen’s 16S V1-V3 Library Prep Kit for Illumina is shipped as one kit box (for the 24 prep kit) or two sub-component kits (for the 96 prep kit). All kits should be stored at -20°C upon arrival.
All kit components should remain stable for at least 1 year when stored at the specified storage conditions.
Malaria is a mosquito-borne infectious disease and considered one of the leading causes of death in the world. Malaria is a fatal tropical disease that is caused by a parasite knows as Plasmodium. It is usually spread through the bite of an infected female mosquito. 300-500 million new infected cases are estimated every year with 1.5-2.7 million deaths worldwide. Most of the malaria-related deaths occur in sub-Saharan Africa. . Although blood is the sample most frequently used to make a diagnosis, both saliva and urine have been investigated as alternative, less invasive specimens
Malaria TaqMan PCR Kit, 100 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
Malaria TaqMan PCR Probe/Primer Set and Controls, 100 reactions
Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
